A method for separating and extracting phenolic glycosides from nigella sativa seeds

A technology of compounds and phenolic glycosides, applied in the field of plant extraction and separation, to achieve the effects of easy elution and regeneration, easy-to-obtain products, and good organic selectivity

Active Publication Date: 2021-10-15
HENAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, there is no report on the isolation and extraction of phenolic glycosides (such as 3,5-dihydroxyphenethyl alcohol-3-O-β-pyranoside, broadbactin C, etc.) from Nigella sativa seeds

Method used

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  • A method for separating and extracting phenolic glycosides from nigella sativa seeds
  • A method for separating and extracting phenolic glycosides from nigella sativa seeds
  • A method for separating and extracting phenolic glycosides from nigella sativa seeds

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Embodiment 1

[0031] A method of separating extracting phenolic compounds (3,5 dihydroxy phenylethyl alcohol-3-o-β-β-pyride, squeezine C) from Nigella Sativa seed seeds, including the steps of:

[0032] 1) Take 5kg dry Nigella Sativa seed as raw materials, pulverized with 7L petroleum ether soaked with 7L petroleum ether with 7L petroleum ether, 3 times per degrease time), residue to smooth the petroleum ether, cold in 70% ethanol Exception 3 times (addition ratio of residue and 70% ethanol), 5kg: 7L), 3 days, combined with extract, recovered solvent, and extract the extract.

[0033] 2) Dissolve the extract to dissolve with methanol, mix in the silica gel column, and use the volume ratio of 20: 1, 15: 1, 10: 1, 5: 1, 3: 1, 1: 1 dichloromethane - Methanol is gradient eluting elut elut elut elut elut elut elut elut elut elut elut elut

[0034] 3) The fourth component FR4 is separated by silica gel column chromatography, with a volume ratio of 30: 1, 20: 1, 15: 1, 10: 1, 5: 1, 1: 1 of dichloromet...

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Abstract

The present invention relates to a Nigella sativa Method for separating and extracting phenolic glycosides from seeds: drying Nigella sativa After the seeds are crushed, degrease with petroleum ether, extract with 60-80% ethanol, recover the solvent under reduced pressure, and obtain the extract extract; Eluted to obtain 7 components Fr1-Fr7; 3) The fourth component Fr4 was separated by silica gel column chromatography to obtain 5 components 4-1~4-5; 4) Component 4-3 was separated by gel column Chromatography, silica gel column chromatography separation, obtain 3,5 dihydroxyphenethyl alcohol-3- O - β ‑Pyranoside; Component 4‑2 was separated by reverse phase column chromatography and Sephadex LH‑20 dextran gel column chromatography to obtain broadbactin C. The extraction process of the invention is simple, the product is easy to obtain, the solvent can be recycled, the extraction amount is large, and industrial production is possible.

Description

Technical field [0001] The present invention belongs to the field of plant extraction separation, and it is specifically involved in the effectively and rapid separation of phenolic compounds (such as 3,5 dihydroxy phenylethyl alcohol-3-o-β-pyranoside) from NiGella Sativa seeds (such as 3,5 dihydroxy phenyl). Method of c, et al. Background technique [0002] Nigella Sativa seeds were seeded as a ranculus black grass plant Nigella Sativa dry seed, which was originally ranked in southwestern Asia, widely distributed in the Mediterranean, Central Europe, West Asia and other places; its seed oil as edible oil in the Middle East and North Africa The Egyptian area is widely used and also used in health food against disease, and is widely used in food. The main components of Nigellasativa seeds have flavonoids, saponins, alkaloids, steroids, and phenols, etc., and also contain a lot of grease. Nigella Sativa seeds have a variety of pharmacological effects, including antibacterial, antio...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H15/203C07H1/08
CPCC07H1/08C07H15/203
Inventor 刘振花康文艺张岩马常阳牛云周莉陈思潭蒙丽君
Owner HENAN UNIVERSITY
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