34results about How to "For in-depth research" patented technology

The invention discloses a preparation method of a ddx19 gene-deleted zebrafish mutant. The preparation method comprises the following steps: determining a target for ddx19 gene knockout on a first exon of a zebrafish ddx19 gene, and designing a gRNA sequence; with a pUC19--gRNA scaffold plasmid as a template, performing PCR amplification by using primers T7-ddx19-sfd and tracr rev; performing purification and in-vitro transcription on a PCR products to obtain gRNA; introducing gRNA and Cas9 protein into a zebrafish embryo-cell stage, and culturing to obtain a ddx19 gene mutant capable of achieving stable inheritance. By a CRISPR / Cas9 technology, the ddx19 gene-deleted zebrafish mutant capable of achieving stable inheritance is obtained. The invention further discloses a phenotype of the ddx19 gene-deleted zebrafish mutant, and a basic support is provided for biological function research and disease modeling thereof.

The invention discloses a preparation method of a ddx27 gene deleted zebra fish mutant. The method comprises steps as follows: the target with a ddx27 gene knocked out is located on the sixth exon ofa ddx27 gene of zebra fish, and a gRNA sequence is designed; Puc19--gRNA scaffold plasmid is used as a template, and primers T7-ddx27-sfd and tracr rev are used for PCR amplification; gRNA is obtainedthrough purification and in-vitro transcription of a PCR product; gRNA and Cas9 protein are imported into the zebra fish, culture is performed, and a stably inherited ddx27gene mutant is obtained. Besides, the invention also discloses a phenotype of the ddx27 gene deleted zebra fish mutant, which plays an important role in research of biological function of the mutant.

A seal performance comprehensive testing device for a deep lunar soil seal assembly relates to a seal performance comprehensive testing device. At present, there is no special seal assembly performance testing device, thus bringing inconvenience for smooth collection and research of deep lunar soil. Therefore, in order to solve the above problem, the invention provides the seal performance comprehensive testing device. The device provided by the invention comprises a seal environmental simulation module (A), a seal force application and test module (B) and a seal collapsing force application and test module (C). The seal force application and test module (B) and the seal collapsing force application and test module (C) are both arranged on the seal environmental simulation module (A). Quantitative application and real-time monitoring of normal closing force are realized through the seal force application and test module. Collapsing force required by destruction of a seal and changes of the collapsing force during the seal destroying process are measured by the seal collapsing force application and test module. The seal performance comprehensive testing device provided by the invention is suitable for testing of the deep lunar soil seal assembly.

The invention discloses an experimental metal / water reaction hydrogen production device and a metal / water reaction hydrogen production collection method and belongs to the technical field of experimental hydrogen production devices. The experimental device comprises a solution storing mechanism, a reacting mechanism and a hydrogen volume detecting mechanism, wherein the solution storing mechanism is communicated with the reacting mechanism, and the reacting mechanism is communicated with the hydrogen volume detecting mechanism. A thermostatic water bath tank is arranged above a magnetic stirrer, a three-opening beaker flask is arranged in the thermostatic water bath tank and provided with three openings including a solution opening, a metal powder opening and a hydrogen opening, the solution opening is communicated with the solution storing mechanism, the metal powder opening is sealed by a sealing plug, and the hydrogen opening is communicated with the hydrogen volume detecting mechanism. A fixing support is fixed above the magnetic stirrer, fixedly connected with the three-opening beaker flask and used for fixing the three-opening beaker flask during reaction. The experimental metal / water reaction hydrogen production device and the metal / water reaction hydrogen production collection method have the advantages that hydrogen collection and automatic recording of produced hydrogen volume are simplified.

The present invention relates to a Nigella sativa Method for separating and extracting phenolic glycosides from seeds: drying Nigella sativa After the seeds are crushed, degrease with petroleum ether, extract with 60-80% ethanol, recover the solvent under reduced pressure, and obtain the extract extract; Eluted to obtain 7 components Fr1-Fr7; 3) The fourth component Fr4 was separated by silica gel column chromatography to obtain 5 components 4-1~4-5; 4) Component 4-3 was separated by gel column Chromatography, silica gel column chromatography separation, obtain 3,5 dihydroxyphenethyl alcohol-3- O - β ‑Pyranoside; Component 4‑2 was separated by reverse phase column chromatography and Sephadex LH‑20 dextran gel column chromatography to obtain broadbactin C. The extraction process of the invention is simple, the product is easy to obtain, the solvent can be recycled, the extraction amount is large, and industrial production is possible.

The invention discloses a method for directly measuring the mechanical properties of rubber reinforcement. 1) Preparation of required samples; 2) Measurement of samples: 2.1) The measurement is carried out on an electronic peeling tester. Clear the force value, displacement and peak value, and set the test-related parameters at the same time; 2.2) Fix the thin rubber strip sample prepared in step 1) on the electronic peel tester with a fixture, and run it; 2.3) After pulling the sample Stop running after breaking, and record the yield strength, elongation rate, tensile strength and maximum force value during the process; 2.4) Prepare the floc sample into a cylindrical or cuboid sample > 6mm, and measure it under the Shore hardness tester its hardness value. The method can directly measure the mechanical properties of the bonded glue, which is convenient for further in-depth research on the bonded glue, and the measurement method is simple and convenient to operate, and users can learn quickly.

The invention relates to a device for detecting the partial discharge signal of an insulating structure, which is characterized by comprising a booster transformer. A full-bridge rectifying circuit is connected with the secondary side of the booster transformer; the output end of the full-bridge rectifying circuit is connected with an H-bridge inverting circuit and an IGBT (insulated gate bipolar transistor) control circuit in a bridging way; the test voltage obtained through the output side of the H-bridge inverting circuit is applied to two ends of a test sample; two input ends of a signal collecting and processing plate are connected with the output end of the H-bridge inverting circuit and the output end of the IGBT control circuit; and the output end of the signal collecting and processing plate is connected with a computer. The circuits of the device have simple structures and can effectively capture and separate the weak signal so that the weak signal can not be submerged in the peak voltage, and reliable data support is provided for the study of the partial discharge of the insulating structure.

The invention discloses a preparation method of a zebrafish ddx27 gene deletion mutant, comprising the following steps: confirming that the target of the ddx27 gene knockout is on the sixth exon of the zebrafish ddx27 gene, and designing a gRNA sequence; using pUC19‑‑‑ The gRNA scaffold plasmid was used as the template, and primers T7‑ddx27‑sfd and tracr rev were used for PCR amplification; the PCR product was purified and transcribed in vitro to obtain gRNA; the gRNA and Cas9 protein were introduced into zebrafish and cultured to obtain a stable genetic mutant of the ddx27 gene. In addition, the invention also discloses the phenotype of the ddx27 gene deletion zebrafish mutant, which plays an important role in studying its biological function.

The invention relates to a mold structure for plate springback experiments that can realize switching of various stamping processes. The mold structure is arranged up and down by an upper mold and a lower mold; the upper mold includes an upper mold base, an upper mold pressing plate, and a concave mold insert; The two die inserts are symmetrically fixed on the left and right sides of the center of the lower end of the upper die base. Block, lower die insert, punch; two lower die inserts are symmetrically fixed on the upper end of the blank holder ring on the left and right sides of the punch; the punch is fixed on the bottom plate of the punch, and the punch and the punch insert are fixedly connected; the punch The insert is set coaxially with the pressing plate of the upper die, and the blank holder can be lifted up by the machine; the mold structure is drawn and formed through the relative movement between the pressing plate of the upper die and the insert of the punch die, and the insert of the die and the insert of the lower die Or flanging stamped sheet. The invention uses a set of mold structure to realize the stamping springback experiment of plates with different thicknesses by three processes of drawing, forming and flanging.

A seal performance comprehensive testing device for a deep lunar soil seal assembly relates to a seal performance comprehensive testing device. At present, there is no special seal assembly performance testing device, thus bringing inconvenience for smooth collection and research of deep lunar soil. Therefore, in order to solve the above problem, the invention provides the seal performance comprehensive testing device. The device provided by the invention comprises a seal environmental simulation module (A), a seal force application and test module (B) and a seal collapsing force application and test module (C). The seal force application and test module (B) and the seal collapsing force application and test module (C) are both arranged on the seal environmental simulation module (A). Quantitative application and real-time monitoring of normal closing force are realized through the seal force application and test module. Collapsing force required by destruction of a seal and changes of the collapsing force during the seal destroying process are measured by the seal collapsing force application and test module. The seal performance comprehensive testing device provided by the invention is suitable for testing of the deep lunar soil seal assembly.

The invention discloses a multimodal brain physiological monitoring system, comprising a signal buffer preprocessing unit (3), a host computer (4), a signal synchronization module (10), a laser speckle blood flow imaging acquisition unit (21), a blood Oxygen information collection unit (22) and electrophysiological collection unit (23), reach control corresponding collection unit work through signal synchronous module (10) while, the signal receiving end corresponding to this collection unit in the buffer area is in the signal buffering control signal Driven and working at the same time to ensure the unity of the signal, the invention can continuously monitor the physiological changes of the brain in time-sharing, and save the collected multi-modal data with the upper computer at the end, which is convenient for further in-depth research in the later stage. At the same time, the present invention can avoid the problem that the acquisition unit directly communicates with the upper computer, which causes disordered data format and complicated signal interface.

The present invention relates to a Nigella sativa Extracted from the seeds to isolate the compound ethyl‑ α ‑D‑Arabinofuranose Method: Choose Dried Nigella sativa Seeds are used as raw materials, crushed and degreased with petroleum ether, extracted with ethanol at room temperature, dissolved in methanol, mixed with silica gel and loaded, eluted with dichloromethane and methanol to obtain Fr1‑Fr77 components, and then through continuous separation and purification, the pure compound is obtained. The method of the present invention is conducive to better development and utilization Nigella sativa This medicinal plant is conducive to the sustainable utilization of plants; the extraction process of the present invention is simple, and it is easy to obtain ethyl- α ‑D‑arabinofuranose compound monomer, the solvent can be recycled, the extraction amount is large, and it can be produced industrially.

The invention provides a method for preparing surface enhanced Raman substrate, and relates to Raman spectrums. The method comprises the steps that a seed crystal growing method is used for synthetizing a gold nanorod solution, wherein gold nano seeds are synthetized first, then a growing solution is added, silver nitrate is used for adjusting the slenderness ratio of gold nanorods until reaction is completed, the needed gold nanorod solution is obtained, then centrifuging is conducted to remove supernatant liquor, signal molecules are added to be connected to a gold surface, then a polyethylene glycol dressing agent is added to induce the gold nanorods to conduct self-assembling, then a processed nano particle solution is stirred, a TEOS methanol solution is added for reacting, silicon dioxide layers are wrapped on the surfaces of the self-assembled gold nanorods, and the surface enhanced Raman substrate is obtained. Detecting can be directly conducted in the solution, due to self-assembling and the coupling effect of plasma on the surfaces of adjacent nano particles, the electromagnetic field between the nano particles is obviously enhanced, the Raman signals at the position are amplified in a quantity level mode, accordingly, the detecting sensitivity of surface enhanced Raman is greatly improved, and the stability is high.

The invention discloses a preparation method of an apple flower effective part, wherein the preparation method comprises the following steps: crushing apple flowers, carrying out heating extraction with ethanol, concentrating the extract liquid, then dispersing in water, and successively extracting with petroleum ether, ethyl acetate and n-butanol, to obtain an ethyl acetate extract. The inventionalso discloses a method for extracting kaempferol glucoside from the apple flower effective part; the apple flower effective part is treated by a silica gel column, a gel column, a preparative high performance liquid phase and other separation methods. The invention provides the method for extracting and separating kaempferol glucoside from the apple flowers, better development and utilization ofthe medicinal plant of the apple flowers are facilitated, kaempferol glucoside is studied deeply, the time and the labor are saved, and the yield of the sample can be greatly improved.

The invention discloses a human gastric cancer taxol drug resistance cell HGC-27 / PTX and a preparation method of the human gastric cancer taxol drug resistance cell HGC-27 / PTX. The human gastric cancer taxol drug resistance cell HGC-27 / PTX is preserved in the China General Microbiological Culture Collection Center (CGMCC), and is assigned with the accession number of CGMCC No.13596. The human gastric cancer taxol drug resistance cell HGC-27 / PTX provided by the invention is stable in property, and can realize a plurality of times of passage stably, so that the in-depth study can be carried out on a molecular mechanism causing cellular drug resistance, and the human gastric cancer taxol drug resistance cell HGC-27 / PTX is an ideal model for studying the drug resistance mechanism of the patient suffering from gastric cancer, and has a significant application value.