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The invention discloses a preparation method of a zebrafish ddx27 gene deletion mutant, comprising the following steps: confirming that the target of the ddx27 gene knockout is on the sixth exon of the zebrafish ddx27 gene, and designing a gRNA sequence; using pUC19‑‑‑ The gRNA scaffold plasmid was used as the template, and primers T7‑ddx27‑sfd and tracr rev were used for PCR amplification; the PCR product was purified and transcribed in vitro to obtain gRNA; the gRNA and Cas9 protein were introduced into zebrafish and cultured to obtain a stable genetic mutant of the ddx27 gene. In addition, the invention also discloses the phenotype of the ddx27 gene deletion zebrafish mutant, which plays an important role in studying its biological function.

A seal performance comprehensive testing device for a deep lunar soil seal assembly relates to a seal performance comprehensive testing device. At present, there is no special seal assembly performance testing device, thus bringing inconvenience for smooth collection and research of deep lunar soil. Therefore, in order to solve the above problem, the invention provides the seal performance comprehensive testing device. The device provided by the invention comprises a seal environmental simulation module (A), a seal force application and test module (B) and a seal collapsing force application and test module (C). The seal force application and test module (B) and the seal collapsing force application and test module (C) are both arranged on the seal environmental simulation module (A). Quantitative application and real-time monitoring of normal closing force are realized through the seal force application and test module. Collapsing force required by destruction of a seal and changes of the collapsing force during the seal destroying process are measured by the seal collapsing force application and test module. The seal performance comprehensive testing device provided by the invention is suitable for testing of the deep lunar soil seal assembly.

The invention discloses a preparation method of an apple flower effective part, wherein the preparation method comprises the following steps: crushing apple flowers, carrying out heating extraction with ethanol, concentrating the extract liquid, then dispersing in water, and successively extracting with petroleum ether, ethyl acetate and n-butanol, to obtain an ethyl acetate extract. The inventionalso discloses a method for extracting kaempferol glucoside from the apple flower effective part; the apple flower effective part is treated by a silica gel column, a gel column, a preparative high performance liquid phase and other separation methods. The invention provides the method for extracting and separating kaempferol glucoside from the apple flowers, better development and utilization ofthe medicinal plant of the apple flowers are facilitated, kaempferol glucoside is studied deeply, the time and the labor are saved, and the yield of the sample can be greatly improved.

The invention discloses a human gastric cancer taxol drug resistance cell HGC-27 / PTX and a preparation method of the human gastric cancer taxol drug resistance cell HGC-27 / PTX. The human gastric cancer taxol drug resistance cell HGC-27 / PTX is preserved in the China General Microbiological Culture Collection Center (CGMCC), and is assigned with the accession number of CGMCC No.13596. The human gastric cancer taxol drug resistance cell HGC-27 / PTX provided by the invention is stable in property, and can realize a plurality of times of passage stably, so that the in-depth study can be carried out on a molecular mechanism causing cellular drug resistance, and the human gastric cancer taxol drug resistance cell HGC-27 / PTX is an ideal model for studying the drug resistance mechanism of the patient suffering from gastric cancer, and has a significant application value.