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Hyaluronic acid-sodium alginate composite hydrogel and preparation method thereof

A composite hydrogel and sodium alginate technology, applied in the field of hyaluronic acid-sodium alginate composite hydrogel and its preparation, to achieve improved mechanical properties and texture characteristics, improved physical and chemical properties, and the ability to resist enzymatic degradation Improved effect

Inactive Publication Date: 2011-05-25
INST OF AGRO FOOD SCI & TECH CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Compounds of SAL and other polysaccharides, such as chitosan, chondroitin sulfate, etc., have been reported, and some people have conducted rheological investigations on the compound of SAL-HA, but this compound is only two kinds of polymers. mixture of substances

Method used

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  • Hyaluronic acid-sodium alginate composite hydrogel and preparation method thereof
  • Hyaluronic acid-sodium alginate composite hydrogel and preparation method thereof

Examples

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Effect test

Embodiment 1

[0021] Example 1. Preparation of hyaluronic acid-sodium alginate hydrogel and verification of its physical and chemical properties

[0022] according to figure 1 The shown process flow chart prepares hyaluronic acid-sodium alginate hydrogel, and the specific steps are as follows:

[0023] ①Polysaccharide dissolution: molecular weight 1.5×10 6 Da's hyaluronic acid (Shandong Freda Biochemical Co., Ltd.) and sodium alginate (a particle size of 200 mesh, 10 mg / ml aqueous solution, the viscosity at 25 ° C is 205 mPa s, Qingdao Jingyan Biotechnology Co., Ltd. company) was dissolved in water at a ratio (mass ratio) of 1:2 so that its total concentration was 1.0% (g / 100ml) to obtain solution 1.

[0024] ②Add a cross-linking agent and adjust the pH of the solution: After the polysaccharide in step ① is completely dissolved, add the cross-linking agent ADH (Beijing Jinyuan Chemical Group Co., Ltd.) into the solution 1 at a ratio of 0.52g:100ml, mix uniform. The pH of the above mixed...

Embodiment 2

[0039] Example 2. Preparation of hyaluronic acid-sodium alginate hydrogel and verification of its physical and chemical properties

[0040] according to figure 1 The shown process flow chart prepares hyaluronic acid-sodium alginate hydrogel, and the specific steps are as follows:

[0041] ① Polysaccharide dissolution: molecular weight 5.0×10 5 Da's hyaluronic acid (Shandong Freda Biochemical Co., Ltd.) and sodium alginate (a particle size of 50 mesh, 10 mg / ml aqueous solution, the viscosity at 25 ° C is 100 mPa s, Qingdao Jingyan Biotechnology Co., Ltd. company) was dissolved in water at a ratio (mass ratio) of 1:4 so that its total concentration was 0.5% (g / 100ml) to obtain solution 1.

[0042] ②Add a cross-linking agent and adjust the pH of the solution: After the polysaccharide in step ① is completely dissolved, add the cross-linking agent ADH (Beijing Jinyuan Chemical Group Co., Ltd.) into the solution 1 at a ratio of 0.1g:100ml, mix uniform. The pH of the above mixed ...

Embodiment 3

[0054] Example 3. Preparation of hyaluronic acid-sodium alginate hydrogel and verification of its physical and chemical properties

[0055] according to figure 1 The shown process flow chart prepares hyaluronic acid-sodium alginate hydrogel, and the specific steps are as follows:

[0056] ① Polysaccharide dissolution: molecular weight 2.1×10 6 Da's hyaluronic acid (Shandong Freda Biochemical Co., Ltd.) and sodium alginate (a particle size of 300 mesh, 10mg / ml aqueous solution, the viscosity at 25 ° C is 300mPa s, Qingdao Jingyan Biotechnology Co., Ltd. company) was dissolved in water at a ratio (mass ratio) of 4:1 so that its total concentration was 1.5% (g / 100ml) to obtain solution 1.

[0057] ②Add a cross-linking agent and adjust the pH of the solution: After the polysaccharide in step ① is completely dissolved, add the cross-linking agent ADH (Beijing Jinyuan Chemical Group Co., Ltd.) into the solution 1 at a ratio of 1.0g:100ml, mix uniform. The pH of the above mixed s...

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Abstract

The invention discloses hyaluronic acid-sodium alginate composite hydrogel and a preparation method thereof. The composite hydrogel is prepared from the following steps of: (1) dissolving hyaluronic acid and sodium alginate into water to obtain a solution 1; (2) adding a cross-linking agent to the solution 1, and regulating the pH value to be 4.0-5.5 with a buffer solution to obtain a solution 2; (3) adding a carboxyl activating agent to the solution 2 and stirring; and regulating and controlling the pH value of the solution 2 to be 2.0-5.5 in the stirring process until gel is formed to obtain the hyaluronic acid-sodium alginate composite hydrogenl. The invention provides a new approach for the modification of the hyaluronic acid, and the composite modified compound prepared by using the method has the advantages of higher mechanical property, capacity of resisting enzymatic degradation of the hyaluronic acid, biocompatibility, and the like and has potential application in the aspects of surgical operations and tissue engineering.

Description

technical field [0001] The invention relates to a hyaluronic acid-sodium alginate composite hydrogel and a preparation method thereof. Background technique [0002] Hyaluronic acid (HA) is a linear, unbranched acidic mucopolysaccharide composed of N-acetylglucosamine and D-glucuronic acid alternately. It is a valuable raw material for medicine and cosmetics. HA has good hydrophilicity, biocompatibility and moisturizing function. It is widely used in cosmetics and is known as a natural moisturizing factor. HA also has good biocompatibility and does not produce rejection in the body. Due to HA's unique rheological properties, lubricity and biocompatibility and other physical and chemical properties, HA can be used as an elastic material in eye surgery, treatment of bone and joint diseases, and has the effect of promoting wound healing and certain anticancer effects. HA also plays an important role in drug sustained release, postoperative anti-adhesion and tissue engineering....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J3/075C08J3/24C08B37/04C08B37/08C08L5/04C08L5/08
Inventor 王强陈永浩刘红芝刘丽
Owner INST OF AGRO FOOD SCI & TECH CHINESE ACADEMY OF AGRI SCI
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