Method for preparing gingko protein peptide
A technology of ginkgo protein and ginkgo, which is applied to the protein composition of vegetable seeds, protein food processing, vegetable protein processing, etc., can solve the problem of low utilization rate and added value of ginkgo nutrient components, insufficient utilization, and low utilization rate of ginkgo biloba and other problems, to achieve the effect of good flavor, saving raw materials and saving time
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Embodiment 1
[0028]Take ripe and fresh ginkgo nuts, shell them to get kernels, put the kernels in boiling water at 100°C for 2 minutes, scald them in hot water at 50-60°C, scrub gently to rinse off the endocarp, and use Rinse with running water, then quickly immerse in an aqueous solution containing 0.5% salt, 0.25% citric acid and 0.1% ascorbic acid for color protection treatment for 30 minutes, remove and drain the water; then coarsely grind the ginkgo nuts with a grinding wheel When coarsely grinding, add 8 times the weight of the nuts in an aqueous solution containing 0.3% salt and 0.2% citric acid, and after grinding into a uniform slurry, immediately send it to the colloid mill for fine grinding, and finely grind for 15-25 minutes to make the pulp Become a uniform milky slurry, and then send the milky slurry into a centrifugal filter for centrifugal filtration to obtain a filtrate, then add 0.3% salt and 0.2% citric acid aqueous solution containing 0.3% salt and 0.2% citric acid solut...
Embodiment 2
[0030] Take the ginkgo powder obtained in Example 1 and pure water according to its weight ratio of 1:20 and mix the ingredients, fully stir, adjust the pH value to between 6.6-7.2 with 1moL / L sodium hydroxide solution, soak for 3h, filter, The filter residue is used for other purposes, take the filtrate and adjust the pH value between 4-5 with 0.5moL / L hydrochloric acid, boil for 3 hours, let it stand for precipitation, separate and remove the upper layer of foam and solution to obtain a precipitate, and then add the precipitate by weight to 5 Double the amount of pure water, stir evenly, and when cooled to 38-45°C, centrifuge and dry to obtain wet ginkgo protein, and obtain ginkgo protein powder after vacuum drying.
Embodiment 3
[0032] Take the ginkgo protein powder prepared in Example 2, add purified water to dissolve, the weight concentration of ginkgo protein powder in water is 2%, heat while stirring constantly, make the solution temperature reach 50 ° C, and use 1moL / L sodium hydroxide Adjust the pH value to 6.5-6.9. After dissolving, measure the pH value again to ensure that the pH value is in the range of 6.6-6.8 and the solution temperature is in the range of 48-52°C. Then add neutral protease at 1.5-3% of the weight of the ginkgo protein solution Carry out enzymatic hydrolysis, the enzymatic hydrolysis time is 2.5-4 hours, to obtain the neutral protease hydrolyzate, and then keep the neutral protease hydrolyzate under the condition of not adjusting the pH value, keep warm at 48-52°C, add ginkgo protein powder The compound flavor protease with a solution weight of 1.5-3% continues to carry out enzymolysis for 4.5-8.5 hours to obtain an enzymolysis solution, and then adjust the pH value of the e...
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