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Method for preparing gingko protein peptide

A technology of ginkgo protein and ginkgo, which is applied to the protein composition of vegetable seeds, protein food processing, vegetable protein processing, etc., can solve the problem of low utilization rate and added value of ginkgo nutrient components, insufficient utilization, and low utilization rate of ginkgo biloba and other problems, to achieve the effect of good flavor, saving raw materials and saving time

Active Publication Date: 2014-03-05
徐州绿之野生物食品有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Ginkgo is rich in protein and starch, which are discarded as waste during the extraction process of ginkgo products, causing a waste of resources. Furthermore, the processing and utilization of ginkgo kernels are mostly low-end ordinary edible products. The utilization rate and added value of nutritional components are low, resulting in waste of resources
[0004] Prior art such as Chinese patent CN102422976A discloses a preparation method of ginkgo polypeptide, its technical feature is that after the shelled ginkgo fruit is extracted with alcohol, after coarse grinding and fine grinding to obtain slurry, a 500kDa ultrafiltration device is used to Ginkgo peptide hydrolyzate was obtained by removing impurities, and then separated by a 20kDa ultrafiltration membrane, then separated by a 100Da nanofiltration membrane, concentrated, and vacuum-dried at 50°C to obtain ginkgo peptide powder , as can be seen from the prior art case, the process is complicated, the yield is low, and the production cost is high. However, the undecomposed protein and starch are still filtered out as waste in the secondary membrane separation and cannot be fully utilized. The utilization rate of ginkgo is relatively low, which cannot bring better utilization efficiency and economic benefits to the development and utilization of ginkgo

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028]Take ripe and fresh ginkgo nuts, shell them to get kernels, put the kernels in boiling water at 100°C for 2 minutes, scald them in hot water at 50-60°C, scrub gently to rinse off the endocarp, and use Rinse with running water, then quickly immerse in an aqueous solution containing 0.5% salt, 0.25% citric acid and 0.1% ascorbic acid for color protection treatment for 30 minutes, remove and drain the water; then coarsely grind the ginkgo nuts with a grinding wheel When coarsely grinding, add 8 times the weight of the nuts in an aqueous solution containing 0.3% salt and 0.2% citric acid, and after grinding into a uniform slurry, immediately send it to the colloid mill for fine grinding, and finely grind for 15-25 minutes to make the pulp Become a uniform milky slurry, and then send the milky slurry into a centrifugal filter for centrifugal filtration to obtain a filtrate, then add 0.3% salt and 0.2% citric acid aqueous solution containing 0.3% salt and 0.2% citric acid solut...

Embodiment 2

[0030] Take the ginkgo powder obtained in Example 1 and pure water according to its weight ratio of 1:20 and mix the ingredients, fully stir, adjust the pH value to between 6.6-7.2 with 1moL / L sodium hydroxide solution, soak for 3h, filter, The filter residue is used for other purposes, take the filtrate and adjust the pH value between 4-5 with 0.5moL / L hydrochloric acid, boil for 3 hours, let it stand for precipitation, separate and remove the upper layer of foam and solution to obtain a precipitate, and then add the precipitate by weight to 5 Double the amount of pure water, stir evenly, and when cooled to 38-45°C, centrifuge and dry to obtain wet ginkgo protein, and obtain ginkgo protein powder after vacuum drying.

Embodiment 3

[0032] Take the ginkgo protein powder prepared in Example 2, add purified water to dissolve, the weight concentration of ginkgo protein powder in water is 2%, heat while stirring constantly, make the solution temperature reach 50 ° C, and use 1moL / L sodium hydroxide Adjust the pH value to 6.5-6.9. After dissolving, measure the pH value again to ensure that the pH value is in the range of 6.6-6.8 and the solution temperature is in the range of 48-52°C. Then add neutral protease at 1.5-3% of the weight of the ginkgo protein solution Carry out enzymatic hydrolysis, the enzymatic hydrolysis time is 2.5-4 hours, to obtain the neutral protease hydrolyzate, and then keep the neutral protease hydrolyzate under the condition of not adjusting the pH value, keep warm at 48-52°C, add ginkgo protein powder The compound flavor protease with a solution weight of 1.5-3% continues to carry out enzymolysis for 4.5-8.5 hours to obtain an enzymolysis solution, and then adjust the pH value of the e...

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Abstract

The invention relates to a method for deeply processing gingko, and particularly discloses a method for preparing gingko protein peptide. The method mainly comprises the following steps: (1), processing raw materials; (2), preparing gingko powder; (3), preparing gingko protein powder; (4), carrying out primary enzymolysis; and (5), carrying out secondary enzymolysis to obtain gingko glycopeptides, amino acid balance peptide and gingko polypeptide products. The utilization of gingko resources is expanded, and new resources are provided for developing products with special functions. The method is special and simple in process, and low in production cost; the gingko protein peptide is safe without toxic and side effects; the novel preparation process of the gingko polypeptide product is developed, a new utilization field of the gingko is opened up. The gingko polypeptide prepared by adopting the method provided by the invention can be used as raw material for developing health care foods, food additives and medicine raw materials and excipients.

Description

technical field [0001] The invention relates to a method for deep processing of ginkgo, in particular to a method for preparing ginkgo protein peptide. Background technique [0002] Ginkgo, commonly known as ginkgo, is rich in protein, starch and a variety of physiologically active ingredients. Ginkgo contains 62.4%-67% of starch, 11.3% of protein, 2.6% of fat, 5.2% of sucrose, 1.1% of reducing sugar, 0.26% of nuclear protein and minerals 3%. Ginkgo contains 17 kinds of amino acids, with a total amount of 10.77%. Among them, there are 7 kinds of essential amino acids, with a content of 3.42%, accounting for 31.8% of the total amino acid content, and 9 kinds of medicinal amino acids, with a content of 7.25%, accounting for 67.3% of the total amino acid content. %, 4 kinds of semi-essential amino acids, the content is 1.33%, accounting for 12.3% of the total amino acid content; ginkgo contains 25 kinds of mineral elements, the major elements account for 99.4% of the total min...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23J1/14A23J3/34A23J3/14
Inventor 张志年其他发明人请求不公开姓名
Owner 徐州绿之野生物食品有限公司
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