Nano magnetic material of surface modified boric acid base group, preparation method and application thereof

A nano-magnetic material and boric acid group technology, applied in the direction of iron oxide, iron oxide/iron hydroxide, etc., can solve problems such as cumbersome operation, and achieve the goal of solving difficult analysis, simple and effective synthesis method, good practical value and application prospect Effect

Inactive Publication Date: 2010-03-31
FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Some studies have successfully applied zeolite nanoparticles to the enrichment of trace peptides, but this method req

Method used

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  • Nano magnetic material of surface modified boric acid base group, preparation method and application thereof
  • Nano magnetic material of surface modified boric acid base group, preparation method and application thereof
  • Nano magnetic material of surface modified boric acid base group, preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Embodiment 1 surface modification Cu 2+ Synthesis of Magnetic Materials

[0028] The synthesis of the surface-modified Cu2+ magnetic material is through the following steps:

[0029] Synthesis of superparamagnetic nanoparticles of ferric oxide with amino groups on the surface by hydrothermal method:

[0030] Using 3.0 grams of FeCl3·6H2O as the raw material, 80-90mL of ethylene glycol as the dispersion system, adding 6-12 grams of anhydrous sodium acetate, 10-20 grams of 1,6-hexamethylenediamine, the reaction temperature is 195-200 ° C, The reaction time is 4-6 hours, and the magnetic nanoparticles with amino groups on the surface are generated, and the particle size is 20-100nm;

[0031] Fe will be synthesized by step (1) 3 o 4 Amino magnetic microspheres are repeatedly washed with deionized water to remove water-soluble impurities, and finally the product is vacuum-dried at 40-50°C to obtain black powder; secondly, the surface of Fe3O4 amino magnetic microspheres ...

Embodiment 2

[0033] Example 2 The surface-modified Cu2+ magnetic material is used for peptide enrichment

[0034] (1) Enzymatic hydrolysis of protein solution:

[0035] 100 μg of asialofetuin was dissolved in 50 mM NH4HCO3 solution (pH 8) at a concentration of 50 ng μl-1, denatured with 10 mM dithiothreitol (60 °C, 30 min), and alkylated with 100 mM iodoacetamide ( 37°C, 45min), trypsin was digested at 37°C overnight, and the enzymatic solution was frozen at -80°C until use.

[0036] (2) Separation and enrichment of peptides:

[0037] 100 μL of glycoprotein hydrolyzate with a concentration of 5ng μL-1 was reacted with about 100ng of magnetic nanomaterials for 90 minutes under continuous shaking, and the reaction system was an NH4HCO3 solution with a pH of about 8.5. Then, the material bonded to the glycopeptide after the reaction was washed 2-3 times with NH4HCO3 solution with a pH of about 8.5, 20 μL each time. The rinse solution was blotted dry and eluted with 10 μL of trifluoroacetic a...

Embodiment 3

[0040] Example 3 The magnetic material with surface-modified boronic acid groups is used for the separation of glycoproteins

[0041] Take 2 μL of glycoprotein (1 μg μL-1), mix with 2 μL of non-glycoprotein (1 μg μL-1), and react with nanomaterials in a solution with a pH of about 8.5 under the conditions of Experiment 2, and the obtained eluate is mixed with untreated The original control solution was subjected to 12% SDS-polyacrylic acid electrophoresis separation. The resulting strips were scanned and analyzed after silver staining.

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Abstract

The invention belongs to the technical fields of inorganic materials and analysis, which relates to a magnetic material of surface modified aminobenzene boric acid, a preparation method and an application thereof. In the invention, a magnetic nano material of ferroferric oxide of which the surface is rich in amino groups is synthesized firstly, and surface chemical modification is carried out on the magnetic nano material by aminobenzene boric acid. The synthesis method of the invention is simple and effective, the prepared magnetic material has very good magnetic field inductivity, large specific surface area and strong specificity, is used as a micro-adsorbent and can be directly put into a peptide section without special processing, and the specific separation and enrichment of glycoprotein and glycopeptide can be realized by a simple magnetic field action without centrifugal separation after being complexed on the magnetic nano material. The material has favorable practical value and application prospect in the fields of protein group science and the like.

Description

technical field [0001] The invention belongs to the technical field of inorganic materials and analysis, and relates to nanometer materials, in particular to a nanometer magnetic material with surface-modified boric acid groups and its preparation method and application. Background technique [0002] It is well known that protein composition is diverse and variable, that is, in different cells of the same species or in the same cell at different times, its protein composition is constantly changing, resulting in extremely complex protein composition. At the same time, due to the post-translational modification of proteins, one mRNA often corresponds to multiple proteins, that is to say, the number of proteins is far more than the number of genes, and proteins vary greatly in size, relative abundance, pH and hydrophobicity, especially For post-translational modification proteins, there are many kinds and the abundance is often very low, but they are often the key substances i...

Claims

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Application Information

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IPC IPC(8): C01G49/06
Inventor 邓春晖姚宁姚国平张祥民
Owner FUDAN UNIV
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