Method for promoting gonad maturation of plectropomus leopardus by applying spectrum

Abstract

The invention provides a method for promoting gonad maturation of plectropomus leopardus by applying a spectrum, which is characterized in that breeding systems under different illumination conditions, including full-spectrum, green-spectrum and blue-spectrum illumination conditions, are constructed, and are combined with a factory-like circulating water breeding system to breed the plectropomus leopardus for 60 days. Experimental results show that the blue light and the green light can significantly accelerate the development of male and female gonads, especially the blue light can promote the development of oocytes, and the green light can promote the development of male germ cells. The method effectively shortens the sexual maturity period of the plectropomus leopardus by accurately regulating and controlling the illumination condition, improves the culture efficiency, and provides a theoretical basis and technical support for regulating and controlling the gonad development environment in aquaculture.

Description

Technical Field

[0001] This invention relates to the field of aquatic organism breeding technology, and in particular to a method for promoting gonadal maturation of leopard-gill perch using spectroscopy. Background Technology

[0002] Leopard-gill spiny perch ( Plectropomus leopardus The leopard sea bass (Stichopus japonicus), commonly known as the Eastern Star Sea Bass, belongs to the order Perciformes, family Serranidae, and genus Stichopus. It is a hermaphroditic marine fish. In recent years, the artificial breeding industry of leopard sea bass has developed rapidly in coastal areas of my country, gradually becoming one of the main fish species in local factory farming, forming a relatively complete aquaculture industry chain. With the continuous increase in market demand, the farming of leopard sea bass urgently needs to shorten the sexual maturity cycle in order to reduce farming costs and increase farmed yields. Therefore, it is imperative to take effective measures to improve the efficiency of artificial breeding and factory farming of leopard sea bass.

[0003] In aquaculture, light, as an environmental factor, is widely used to regulate the growth, development, and reproduction of aquatic animals. Existing research has shown that light can affect the gonadal development of fish, and its effect is closely related to the color, intensity, and duration of the light. Leopard sea bass, as a reef fish, is highly sensitive to light, and its gonadal development is easily affected by ambient light. Therefore, light, as an environmental factor, plays an indispensable role in the gonadal development of leopard sea bass. Although breakthroughs have been made in the indoor artificial breeding technology of leopard sea bass, solving its breeding problems to some extent, the specific effects of different colors of light on the gonadal development of leopard sea bass under artificial breeding conditions have not yet been fully studied and reported. Summary of the Invention

[0004] In view of this, the present invention proposes a method for promoting gonadal maturation of leopard-spotted spiny perch using spectroscopy, thereby solving the above-mentioned problems.

[0005] The technical solution of this invention is implemented as follows: A method for promoting gonadal maturation of the leopard-gill perch using spectroscopy, comprising the following steps: (1) Construct a light-based aquaculture system that includes green spectrum irradiation lamps, blue spectrum irradiation lamps, full spectrum irradiation lamps and a circulating water treatment system; (2) Select individuals of the leopard gill spiny perch in the sex differentiation period and place them in this breeding system for breeding; (3) After a specific breeding period, the gonadal development status of the leopard gill spiny perch was tested.

[0006] Preferably, the illumination lamp in step (1) is an LED lamp, which is suspended in the center area at a distance of 1.2-1.8m from the surface of the aquaculture water.

[0007] Preferably, in step (1), the wavelength of green light in the green spectral irradiation lamp is 550-590nm, the wavelength of blue light in the blue spectral irradiation lamp is 460-500nm, and the light intensity at the water surface is controlled at 550-650lx.

[0008] Preferably, in the light-based aquaculture system described in step (1), the aquaculture container has a volume of 400L, and the container is covered with a light-shielding material to isolate external light source interference. The light-shielding material includes, but is not limited to, black canvas.

[0009] Preferably, the leopard gill spiny perch selected in step (2) is an individual in the sex differentiation stage, with no injuries on its body surface and agile movement.

[0010] Preferably, the breeding density in step (2) is 80-100 fish per 400L breeding container.

[0011] Preferably, the light cycle during the breeding process described in step (2) is 12 hours of light and 12 hours of darkness per day.

[0012] Preferably, natural seawater is used in the aquaculture process described in step (2), and the water temperature is maintained at 22-26℃.

[0013] Preferably, the specific breeding cycle in step (3) is 50-70 days.

[0014] Preferably, after the breeding in step (3) is completed, 9 leopard gill spiny perch in good condition are randomly selected from each group, and the gonad tissue is obtained after anesthesia for subsequent testing.

[0015] Compared with the prior art, the beneficial effects of the present invention are: This invention reveals and applies the differential promoting effect of specific light spectrum on the gonadal development of the leopard-gill spiny perch. Experimental results show that blue light specifically accelerates ovarian development, promoting oocyte growth to stages III and IV; while green light specifically accelerates testis development, inducing the production of primary spermatocytes, secondary spermatocytes, and spermatids. This precise regulation of male and female reproductive cell development solves the problem of the lack of effective environmental induction methods in existing technologies. Through specific light regulation, this invention can effectively shorten the gonadal maturation cycle of the leopard-gill spiny perch under natural conditions or conventional artificial breeding conditions. It also sets reasonable ranges for core indicators such as breeding container volume, seedling age, breeding density, light parameters, and water temperature, overcoming the limitations of applying single fixed parameters. Compared with traditional methods, this invention significantly shortens the sexual maturity cycle, directly improving the efficiency of artificial breeding and further optimizing the factory farming process. Attached Figure Description

[0016] Figure 1 Schematic diagram of a light-based rearing system for juvenile leopard gill sea bass; Figure 2 Histological observation of gonads after irradiation with different lights; Among them, A represents the histological observation of the gonads of the leopard-gill spiny perch in the full spectrum group, and a1 and a2 represent facultative gonads; B represents the histological observation of the gonads of the blue-spectrum group of the leopard-gill spiny perch; b1 represents the mature ovary; and b2 represents the facultative gonad. C represents histological observation of the gonads of the leopard-spotted spiny perch in the green spectral group; c1 represents the mature testes; and c2 represents the facultative gonads. D represents the histological observation of the gonads of the red-spectrum group of the leopard-gill spiny perch; d1 and d2 represent facultative gonads. E represents the histological observation of the gonads of the leopard-spotted spiny perch in the group without natural light irradiation; e1 and e2 represent facultative gonads.

[0017] In the diagram, OG represents oogonia; II represents secondary oocytes; III represents tertiary oocytes; IV represents quaternary oocytes; V represents quinary oocytes; SC represents spermatogonia; PS represents primary spermatocytes; SS represents secondary spermatocytes; and ST represents spermatids.

[0018] Figure 3 A statistical chart showing the diameter of oocytes after exposure to different lighting conditions. Detailed Implementation

[0019] To better understand the technical content of this invention, specific embodiments are provided below to further illustrate the invention.

[0020] Unless otherwise specified, the experimental methods used in the embodiments of this invention are all conventional methods.

[0021] Unless otherwise specified, all materials and reagents used in the embodiments of this invention are commercially available.

[0022] Example 1: Constructing aquaculture systems with different lighting conditions like Figure 1 As shown, the aquaculture tank had a volume of 400L and was covered with black canvas to avoid sunlight and other light source interference. All experiments were conducted under natural seawater temperatures of 22-26℃, with a 12-hour light (L) to 12-hour dark (D) illumination cycle (the system automatically controlled the light exposure time, turning it on at 07:00 and off at 19:00). LED lights (manufactured by Foshan Electrical and Lighting Co., Ltd.) were used as the light source, suspended 1.5m above the water surface at the center, providing full-spectrum white light. During the aquaculture experiment, the wavelength and light intensity of the LED lights were regularly measured and calibrated to ensure that the blue light wavelength was 480nm, the green light wavelength was 570nm, and the red light wavelength was 590nm. The light intensity measured at the water surface was approximately 600lx. All experimental conditions were the same except for the light color.

[0023] Example 2: Aquaculture Leopard gill spiny perch selected at the sex differentiation stage, with no external injuries and agile movement, were placed in the breeding system. 100 individuals were placed in each 400L breeding container. The photocycle was 12 hours of light and 12 hours of darkness per day. The total breeding experiment period was 60 days.

[0024] Example 3: Gonadal Development Status of Leopard Gill-Spined Perch under Different Color Spectra 1. Sampling Leopard-gill sea bass in the sex differentiation stage were selected for culture. After 60 days of culture under different color spectra, samples were taken. Nine healthy leopard-gill sea bass from each group were randomly selected, anesthetized, and their gonads were dissected. The fascia and external tissues were removed and the samples were washed with 1×PBS. The samples were placed in 4% paraformaldehyde tissue fixative and incubated at 4°C for 24-48 hours. After graded dehydration with alcohol, the samples were stored in anhydrous ethanol for subsequent paraffin sectioning.

[0025] 2. Paraffin sections Samples fixed in 4% paraformaldehyde for more than 24 hours were removed, and subjected to gradient ethanol dehydration, tissue clearing, tissue embedding, tissue sectioning, and HE staining to observe the gonadal development status after treatment with different color spectra. Figure 2 As shown, histological results indicate that the gonadal development in the full-spectrum group, red-spectrum group, and no-natural-light group was entirely facultative gonadal, with no fully developed germ cells. In the blue-spectrum group, in addition to facultative gonadal development, a more mature ovary was observed. Figure 2 (B-b1), the gonads contain a large number of stage III and IV oocytes, with a significantly increased oocyte diameter. In some individuals of the green spectrum group, male germ cells at different developmental stages were observed in the gonads, including primary spermatocytes, secondary spermatocytes, and spermatids (…). Figure 2 (C-c1). Statistical results on oocyte diameter showed that the oocyte diameter in the blue light group was significantly increased ( Figure 3 The results indicate that blue light promotes oocyte development. The proportion of gonadal development status in different light color groups is shown in Table 1. Both the blue light group and the green light group showed relatively mature individuals.

[0026] Table 1. Statistical analysis of gonadal development status of Leopard Gill-spined Perch under different spectra.

[0027] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A method for promoting gonadal maturation in leopard-gill perch using spectroscopy, characterized in that, Includes the following steps: (1) Construct a light-based aquaculture system that includes green spectrum irradiation lamps, blue spectrum irradiation lamps and a circulating water treatment system; (2) Select individuals of the leopard gill spiny perch in the sex differentiation period and place them in this breeding system for breeding; (3) After a specific breeding period, the gonadal development status of the leopard gill spiny perch was tested.

2. The method for promoting gonadal maturation of leopard-gill perch using spectroscopy as described in claim 1, characterized in that, The illumination lamp in step (1) is an LED lamp, which is suspended in the center area at a distance of 1.2-1.8m from the surface of the aquaculture water.

3. The method for promoting gonadal maturation of leopard-gill perch using spectroscopy as described in claim 1, characterized in that, In step (1), the wavelength of green light in the green spectral irradiation lamp is 550-590nm, the wavelength of blue light in the blue spectral irradiation lamp is 460-500nm, and the light intensity at the water surface is controlled at 550-650lx.

4. The method for promoting gonadal maturation of leopard-gill perch using spectroscopy as described in claim 1, characterized in that, In the light-based aquaculture system described in step (1), the aquaculture container has a volume of 400L. The container is covered with light-shielding material to isolate external light source interference. The light-shielding material includes, but is not limited to, black canvas.

5. The method for promoting gonadal maturation of leopard-gill perch using spectroscopy as described in claim 1, characterized in that, The leopard gill spiny perch selected in step (2) are individuals in the sex differentiation stage, with no injuries on their body surface and agile movement.

6. The method for promoting gonadal maturation of leopard-gill perch using spectroscopy as described in claim 1, characterized in that, The breeding density in step (2) is 80-100 fish per 400L breeding container.

7. The method for promoting gonadal maturation of leopard-gill perch using spectroscopy as described in claim 1, characterized in that, In step (2), the light cycle during the breeding process is 12 hours of light and 12 hours of darkness per day.

8. The method for promoting gonadal maturation of leopard-gill perch using spectroscopy as described in claim 1, characterized in that, In step (2), natural seawater is used in the aquaculture process, and the water temperature is maintained at 22-26℃.

9. The method for promoting gonadal maturation of leopard-gill perch using spectroscopy as described in claim 1, characterized in that, The specific breeding cycle described in step (3) is 50-70 days.

10. A method for promoting gonadal maturation of leopard-gill perch using spectroscopy as described in claim 1, characterized in that, After the breeding process in step (3) is completed, nine healthy leopard gill spiny perch are randomly selected from each group, and their gonads are dissected after anesthesia for further testing.

Images