Tongue sole induced spawning and gynogenesis diploid fish fry induction method

Abstract

The invention relates to an abduction method of a cynoglossus semilaevis diploid fry that is manually induced to be spawned and developed from female nucleus, comprising manually induced spawning of a parent fish, genetic inactivation of sperms, and an induction and identification method of the female nucleus developed diploid fry. The invention firstly establishes a method to obtain large batches of unfertilized ovum by manually induced spawning of the cynoglossus semilaevis, and builds up a method that the cynoglossus semilaevis sperms and lateolabrax japonicus sperms are adopted to induce the developing of the female nucleus of the cynoglossus semilaevis and obtain the female nucleus diploid fry, thus sieving effective manually induced spawning hormones kinds and dosages, and sieving cold shock beginning time and temperature and time that are suitable for the development of the female nucleus of the cynoglossus semilaevis, and the identification method of the female nucleus developed fry of the cynoglossus semilaevis is established. The insemination rate of the ovum of the cynoglossus semilaevis obtained by the method is 60 to 89 percent, the hatch ability is 34 to 79 percent, and the induction rate of the female nucleus developed fry is 0.4 to 6.3 percent. The invention is characterized by advancement, high effect, practicability and reliability, and has important application value and wide popularization prospect on the breeding of fry kinds of the cynoglossus semilaevis, sex control and hologynic breeding.

Description

technical field [0001] The technology belongs to the technical field of fish genetics and breeding, and is a method for batch-producing unfertilized eggs of tongue sole suitable for seed breeding and inducing gynogenesis of tongue sole eggs to obtain gynogenetic diploid fry, which can be applied to tongue sole seedlings large-scale production and production of all-female seedlings. Background technique [0002] Cynoglossus semiliaevis (Cynoglossus semiliaevis) is a rare and valuable seawater fish endemic to my country. It belongs to warm-water demersal fish in coastal waters. It is distributed in coastal areas of my country, mostly in the Yellow Sea and Bohai Sea. Semi-smooth tongue sole is popular among consumers because of its delicious taste, tender meat and rich nutrition. Its market value is extremely high and its breeding prospect is very broad. Semi-smooth tongue sole is one of the main species of marine fish farming at present. Its aquaculture output is currently ne...

AI Technical Summary

Problems solved by technology

However, there are no reports about the artificial gynogenesis technology of semi-smooth tongue sole and the technology of inducing diploid fry through artificial gynogenesis.

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