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Induction and identification method of cynoglossus semilaevis gynogenesis diploid fish fry

A technology for gynogenesis and semi-smooth tongue sole, applied in fish farming, application, climate change adaptation, etc., can solve problems that have not been reported, and achieve broad application prospects, advanced technology, and high-efficiency methods

Inactive Publication Date: 2011-08-31
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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  • Description
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Problems solved by technology

However, there are no reports about the artificial gynogenesis technology of semi-smooth tongue sole and the technology of inducing diploid fry through artificial gynogenesis.

Method used

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  • Induction and identification method of cynoglossus semilaevis gynogenesis diploid fish fry
  • Induction and identification method of cynoglossus semilaevis gynogenesis diploid fish fry
  • Induction and identification method of cynoglossus semilaevis gynogenesis diploid fish fry

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Embodiment Construction

[0037] Taking the semi-smooth tongue sole as an example below, the technical content of the present invention is described in detail:

[0038] The technical contents of the present invention include:

[0039] 1. Induction of gynogenetic diploid fry of semi-smooth tongue sole; 2. Identification method of gynogenetic diploid fry of semi-smooth tongue sole.

[0040] 1. Induction of gynogenetic diploid fry of semi-smooth tongue sole: its technical content includes: 1. Genetic inactivation of sperm; 2. "Fertilization" of unfertilized eggs and inactivated sperm; 3. Chromosome doubling of gynogenetic eggs Determination of the starting time; 4. Determination of the cold shock temperature and treatment time for the chromosome doubling of gynogenetic fish eggs.

[0041] 1. Genetic inactivation of spermatozoa:

[0042] After screening and comparison, the experiment of the present invention shows that both the homologous tongue sole sperm and the heterologous perch sperm can be used to ...

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Abstract

The invention relates to an induction method of a cynoglossus semilaevis gynogenesis diploid fish fry, which comprises a cynoglossus semilaevis ovum artificial gynogenesis method and a gynogenesis fish fry identifying method. The induction method comprises the steps of: firstly, establishing a method for obtaining the gynogenesis diploid fish fry through inducing cynoglossus semilaevis ovum gynogenesis by adopting a cynoglossus semilaevis sperm and a perch sperm; screening to obtain cold shock starting time, cold shock temperature and time of the cynoglossus semilaevis egg gynogenesis; and establishing an indentifying method of the cynoglossus semilaevis gynogenesis fish fry. The induction rate of the gynogenesis fish fry is 0.4-6.3 percent. The invention has the characteristics of advanced technology, high efficiency, practicability and reliability, and has application value and wide popularization and application prospects on cynoglossus semilaevis sex control and full female breeding.

Description

[0001] This application is a divisional application of the patent application "Artificial Occurrence of Semi-smooth Tongue Sole and Induction Method for Gynogenetic Diploid Frylings". The filing date of the original application is: November 1, 2007, and the application number is: 200710114301.7. : Artificial induced spawning and gynogenetic diploid fry of half-smooth tongue sole. technical field [0002] The technology belongs to the technical field of fish genetics and breeding, and is a method for inducing gynogenetic growth of tongue sole eggs to obtain gynogenetic diploid fry, which can be applied to the production of all-female seedlings of tongue sole semismooth. Background technique [0003] Half-smooth tongue sole ( Cynoglossus semiliaevis ) is a rare and valuable economic seawater fish unique to my country. It belongs to offshore warm water bottom fish. It is distributed in my country's coastal areas, mostly in the Yellow Sea and Bohai Sea. Semi-smooth tongue sole ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 陈松林田永胜杨景峰刘海金翟介明邵长伟廖小林苏鹏志季相山徐建勇武鹏飞
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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