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Blood purification adsorption agent for clearing immune body and preparation method thereof

A blood purification and adsorbent technology, applied in chemical instruments and methods, blood circulation treatment, other chemical processes, etc., can solve the problems of insignificant treatment effect, high price of protein A adsorbent, and limited scope of application.

Inactive Publication Date: 2008-05-28
DALIAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose and task of the present invention is to overcome the existing problems of the prior art in this field: (1) protein A adsorbent is expensive, and the cost that the patient needs to bear to buy a domestic adsorption column is as high as 30,000 yuan or more, and the scope of application is limited; (2) The therapeutic efficiency of the adsorbent with hydrophobic amino acid as the ligand is not high, and the amount of antibody removed by a single column is about 2.2g, and the therapeutic effect is not obvious

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Take the gel with a volume of 10mL after fully settling (overnight), wash it with 200mL of anhydrous acetone several times to remove the water contained in the gel, then disperse the agarose gel evenly in an equal volume of acetone, and ensure that the whole system is free of water. water. Weigh 0.8 g of CDI and mix it with the gel suspension, and stir the resulting mixture at 15° C. for 1 hour. After the reaction, the gel was washed several times with 200 mL of anhydrous acetone. A cross-linked agarose gel with imidazole carbamate active groups was obtained, and the degree of substitution was 1.6 mmol / g dry gel according to elemental analysis, and 1 g of dry gel was equivalent to a wet volume of 35 mL.

[0025] 10 mL of CDI-activated gel was evenly dispersed in an equal volume of acetone, and hexamethylenediamine was added to the gel system in a 5-fold excess, and the resulting mixture was suspended and stirred at 20°C for 2 hours. After the reaction, wash with 100 m...

Embodiment 2

[0028] The specific steps are the same as in Example 1, except that the amount of CDI is increased to 1.2 g / 10 mL of wet glue, and the activation reaction is carried out at 30° C. for 2 hours. After the activation, the elemental analysis showed that the degree of substitution was 3.6 mmol / g dry glue. The amount of hexamethylenediamine used in the arm grafting reaction was 10 times excess, and the arm grafting reaction was carried out at 35° C. for 3 hours. When coupling benzoic acid, the reaction was carried out in a pH 6.5 buffer solution at 35° C. for 4 hours. NaOH solution at pH 13 was used for hydrolysis of remaining imidazole activating groups. The coupling density of its product is 2.6mmol / g dry glue through elemental analysis.

Embodiment 3

[0030] The specific steps are the same as in Example 1, except that the amount of CDI is 1.0 g / 10 mL of wet glue, and the activation reaction is carried out at 30° C. for 1 hour. After the activation, the elemental analysis showed that the degree of substitution was 3.1 mmol / g dry glue. The amount of hexamethylenediamine used in the arm grafting reaction was 10-fold excess, and the arm grafting reaction was carried out at 30°C for 2.5 hours. When coupling benzoic acid, the reaction was carried out in a pH 6.5 buffer solution at 30°C for 3 hours. The coupling density of its product is 2.1mmol / g dry glue through elemental analysis.

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Abstract

The invention relates to a blood purification adsorbent which is used for eliminating antibody and the preparation method in the field of biomedical materials. The blood purification adsorbent comprises two parts of vector matrix and ligand, wherein, the ligand is fixed at the vector matrix by chemical coupling. The invention is characterized by taking benzoic acid molecular as the immobilized ligand of the blood purification adsorbent. The preparation method of the blood purification adsorbent takes cross-linking polysaccharide such as agarose, etc. as the vector matrix and prepares the adsorbent material by four steps of activation, arm hook, coupling matrix and post-processing. The invention has the advantages that: the invention can adsorb the antibody components in the plasma with high load; the invention has the adsorption limitation on nonspecific other plasma components such as serum albumin, etc.; the preparation cost is low; and the physicochemical properties are stable. The material can be used for eliminating the self antibody and the immune complex in the plasma as the adsorption filler of a blood purification device.

Description

Technical field: [0001] The invention relates to a blood purification adsorbent for antibody removal in the field of biomedicine and a preparation method thereof. Background technique [0002] Autoantibodies and immune complexes have been proven to be closely related to the generation and development of a series of diseases, such as immune reactions (allergy, transplant rejection), autoimmune diseases (systemic lupus erythematosus, rheumatoid, etc.), and even cancer. Therefore, there is a clinical need for an adsorbent that can selectively remove autoantibodies and circulating immune complexes from human blood, so as to relieve acute symptoms, curb the development of the disease, and then play a therapeutic role in the disease. As the incidence of such diseases, especially autoimmune diseases, increases year by year, this demand is also increasing. Based on the above considerations, many institutions have developed adsorbent materials with different properties, some of whic...

Claims

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Application Information

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IPC IPC(8): B01J20/26A61M1/38
Inventor 贾凌云任军张丹妮谢健
Owner DALIAN UNIV OF TECH
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