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Temperature-regulated phase material microcapsule carrier immobilized enzyme and preparation method for same

A phase change material, a technology for immobilizing enzymes, which is applied in the direction of being immobilized on or in an inorganic carrier, immobilized on/in an organic carrier, etc., and can solve the problem of narrow working temperature range, poor thermal stability and cycle stability, etc. problems, to achieve the effect of low equipment requirements, improved activity recovery rate, and improved stability

Active Publication Date: 2016-09-21
BEIJING UNIV OF CHEM TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to solve the problem of narrow working temperature range and poor storage stability, thermal stability and cycle stability of the traditional carrier immobilized enzyme, and provide a kind of immobilized enzyme with temperature-controlled phase change material microcapsule as the carrier material. The enzyme and its preparation method can significantly improve the stability and activity of the immobilized enzyme by utilizing the characteristic of the microenvironment temperature function of the carrier material, and have great application potential in the field of immobilized enzyme development and application

Method used

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  • Temperature-regulated phase material microcapsule carrier immobilized enzyme and preparation method for same
  • Temperature-regulated phase material microcapsule carrier immobilized enzyme and preparation method for same
  • Temperature-regulated phase material microcapsule carrier immobilized enzyme and preparation method for same

Examples

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Effect test

Embodiment 1

[0039] Step (1): Preparation of temperature-controlled phase change material microcapsule carrier

[0040] First, 1.0g of ferric chloride hexahydrate and 0.4g of ferrous chloride tetrahydrate were dissolved in 100mL of deionized water, heated to 60℃ and kept for 5h, then adjusted to pH=10 with ammonia, and added 0.1g of dodecane Sodium sulfate, continue to stir for 20min in N 2 Hydrophobic magnetic nanoparticles were prepared by reaction under the protection of the atmosphere; 0.4g magnetic nanoparticles, 5.0g n-eicosane and 5.0g n-butyl titanate were added to a three-necked flask, and sonicated at 40°C for 30 minutes to make the magnetic nanoparticles and Disperse the titanium source on the surface of the organic phase change material, then add 60mL formamide solution and 0.5g sodium lauryl sulfate and continue to stir for 2h to form a stable emulsion system; prepare a mixture of 1mL deionized water and 40mL formamide, and slowly Add dropwise to the above emulsion system, contin...

Embodiment example 2

[0044] Step (1): Preparation of temperature-controlled phase change material microcapsule carrier

[0045] First, 1.0g of ferric chloride hexahydrate and 0.4g of ferrous chloride tetrahydrate were dissolved in 100mL of deionized water, heated to 60℃ and kept for 5h, then adjusted to pH=10 with ammonia, and added 0.1g of dodecane Sodium sulfate, continue to stir for 20min in N 2 Hydrophobic magnetic nanoparticles were prepared by reaction under the protection of the atmosphere; 0.4g magnetic nanoparticles, 5.0g n-eicosane and 5.0g n-butyl titanate were added to a three-necked flask, and sonicated at 40°C for 30 minutes to make the magnetic nanoparticles and Disperse the titanium source on the surface of the organic phase change material, then add 60mL formamide solution and 0.5g sodium lauryl sulfate and continue to stir for 2h to form a stable emulsion system; prepare a mixture of 1mL deionized water and 40mL formamide, and slowly Add dropwise to the above emulsion system, contin...

Embodiment example 3

[0049] Step (1): Preparation of temperature-controlled phase change material microcapsule carrier

[0050] First, 1.0g of ferric chloride hexahydrate and 0.4g of ferrous chloride tetrahydrate were dissolved in 100mL of deionized water, heated to 60℃ and kept for 5h, then adjusted to pH=10 with ammonia, and added 0.1g of dodecane Sodium sulfate, continue to stir for 20min in N 2 Hydrophobic magnetic nanoparticles were prepared by reaction under the protection of the atmosphere; 0.4g magnetic nanoparticles, 5.0g n-eicosane and 5.0g n-butyl titanate were added to a three-necked flask, and sonicated at 40°C for 30 minutes to make the magnetic nanoparticles and Disperse the titanium source on the surface of the organic phase change material, then add 60mL formamide solution and 0.5g sodium lauryl sulfate and continue to stir for 2h to form a stable emulsion system; prepare a mixture of 1mL deionized water and 40mL formamide, and slowly Add dropwise to the above emulsion system, contin...

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Abstract

The invention discloses a temperature-regulated phase material microcapsule carrier immobilized enzyme and a preparation method for the same, and relates to the technical field of biology. The preparation method comprises the following steps: preparing a microcapsule carrier obtained by cladding an organic phase change material with a crystalline titanium dioxide / magnetic particle composite material, performing carboxylation modification on the microcapsule carrier, and implementing enzyme immobilization by adopting a chemical coupling method, so as to finally obtain the temperature-regulated phase material microcapsule carrier immobilized enzyme. According to the temperature-regulated phase material microcapsule carrier immobilized enzyme and the preparation method for the same, heat can be stored and released by the microcapsule carrier of the immobilized enzyme through a phase change material capsule core, so that micro-environmental temperature around the carrier can be regulated, the temperature application range of enzymatic reaction can be widened, and the heat stability, the storage stability and the cycling stability of the immobilized enzyme can be enhanced. In addition, the temperature-regulated phase change material microcapsule carrier can be used for immobilizing different types of enzymes, and is wide in application range.

Description

Technical field [0001] The present invention relates to the field of biotechnology, in particular to a phase change material microcapsule carrier immobilized enzyme with automatic microenvironment temperature adjustment and a preparation method thereof. Background technique [0002] Enzymes are macromolecules with catalytic function and are catalysts for all metabolic reactions in organisms. It has the advantages of mild catalytic reaction conditions, high catalytic efficiency, strong substrate specificity, few by-products, and convenient adjustment of catalytic activity. It is an ideal catalyst. However, the free enzyme has the following shortcomings: its structure is easily affected by the external environment and inactivation occurs; it is difficult to separate and recover after use, and it is difficult to achieve continuous utilization. With the emergence of immobilized enzyme technology, the above shortcomings of free enzymes have been overcome, and the application and deve...

Claims

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Application Information

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IPC IPC(8): C12N11/14C12N11/02
CPCC12N11/02C12N11/14
Inventor 汪晓东蒋彬彬武德珍
Owner BEIJING UNIV OF CHEM TECH
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