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O-nitrobenzaldehyde degrading bacteria and use thereof

A technology of o-nitrobenzaldehyde and degrading bacteria, applied in the field of biological treatment of environmental pollutants, can solve the problems of limited application, narrow degradation spectrum, limited number and types of degrading bacteria, etc., and achieve the effect of wide application and broad degradation spectrum

Inactive Publication Date: 2009-04-01
ZHEJIANG FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the number and types of degrading bacteria with nitrobenzaldehyde degrading ability that have been separated are all quite limited, and mainly focus on m-nitrobenzaldehyde (Daniel M., Bernard W., Andreas S.Suitability of recombinantEscherichia coli and Pseudomonas putida strains for selective biotransformation of m-nitrotoluene by xylene monooxygenase. Applied and Environmental Microbiology, 2005, 71(11): 6624-6632)
The only bacterial strain Pseudomonsa putida ONBA-17 with the ability to degrade o-nitrobenzaldehyde (ONBA) is also due to its narrow degradation spectrum (only 5 of the 13 aromatic compounds tested), and it is resistant to about 10 kinds of aromatic compounds. The common antibiotics are quite resistant (it is generally believed that the release of such resistant strains into the natural environment will lead to the spread of resistance factors among indigenous bacteria) and the lack of actual ONBA degradation ability under soil environmental conditions limits its Application (Yu F.B., Shen B., Li S.P. Isolation and characterization of Pseudomonas sp.strain ONBA-17 degrading o-nitrobenzaldehyde. Current Microbiology, 2006, 53: 457-461)

Method used

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  • O-nitrobenzaldehyde degrading bacteria and use thereof
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  • O-nitrobenzaldehyde degrading bacteria and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Embodiment 1: The performance of separation and degradation o-nitrobenzaldehyde of Alcaligenes sp.ND1

[0027] After collecting the activated sludge in the sludge thickening tank of Hangzhou Municipal Sewage Treatment Plant on site, it was packed in a 150mL triangular flask and quickly brought back to the laboratory.

[0028] The retrieved sludge samples were placed in ONBA selective basal inorganic salt liquid medium for acclimation culture. The medium composition is (g / L): Glucose 1.0, NaCl 1.0, K 2 HPO 4 1.5, KH 2 PO 4 0.5, (NH 4 ) 2 SO 4 1. MgSO 4 ·7H 2 O0.2, ONBA 0.1g, add deionized water to make up to 1,000mL, sterilize with moist heat at 115℃ for 30 minutes and set aside. The culture conditions are: 28°C, 150r / min shaking culture, and the liquid volume in the container is 1 / 3 of the container volume. After culturing for 3-5 days, the shake flask was left still for a while, the supernatant was discarded, and then an equal amount of freshly configured sel...

Embodiment 2

[0031] Embodiment 2: Degradation substrate experiment of Alcaligenes sp.ND1

[0032] Inoculate Alcaligenes sp.ND1 to o-nitrobenzaldehyde, naphthalene, p-nitrophenol, o-nitrophenol, o-aminophenol, toluene, p-dimethyl Aminobenzaldehyde, 2,4-dinitrophenol, p-hydroxybenzoic acid, diphenylamine, benzoic acid and dimethylbenzene in the basic inorganic salt culture medium, set up substrate blank control simultaneously (only add substrate and do not add bacteria), After 2 days of shake culture at 150r / min and 30°C in a shaker in the dark, the biomass was measured and the degradation of the substrate was detected. The growth of the strain is shown in Table 2. The results show that the strain ND1 can use a certain concentration of the tested substrate for growth and reproduction, and the tolerance concentration to different substrates is different. The tolerated concentration of the test substrate should be at least 100 mg / L. After cultivating for 2 days, it was observed that the subs...

Embodiment 3

[0040] Example 3: Antibiotic susceptibility experiment of Alcaligenes sp.ND1

[0041] The antibiotic susceptibility test adopts the filter paper method, and five commonly used antibiotics such as ampicillin, tetracycline, chloramphenicol, kanamycin and streptomycin are selected, and the diameter of the inhibition zone of each antibiotic filter paper on the ND1 plate for cultivating degrading bacteria , as a criterion for judging sensitivity or resistance. The test results given in Table 3 showed that the strain ND1 was sensitive to the above five antibiotics.

[0042] Table 3. Results of resistance test of Alcaligenes sp.ND1

[0043]

[0044]

[0045] Note: The diameter of the inhibition zone is greater than the upper limit of the moderately sensitive range as sensitive, less than the lower limit as resistant, and within the range as moderately sensitive.

[0046] This example shows that when the bacteria are released into the natural environment, they will not spread ...

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Abstract

The invention pertains to the technical field of bio-treatment of environmental pollutants, particularly relates to a bacterium for degrading o-nitrobenzaldehyde and the applications thereof in bio-treatment of waste water and remediation of soil environmental pollution. The degrading bacterium of o-nitrobenzaldehyde is the strain ND1 of Alcaligenes sp. The bacterium can degrade o-nitrobenzaldehyde with high efficiency under aerobic condition, and utilize albocarbon, p-nitrophenol, o-nitrophenol, ortho-aminophenol, toluene, p-dimethylaminobenzaldehyde, 2, 4-dinitrophenol, para hydroxy benzoic acid, diphenylamine, benzoic acid, xylene and a plurality of other aromatic compounds. The Alcaligenes sp.ND1 is sensitive to five common antibiotics of tetracycline, kanamycin, streptomycin, chloromycetin and ampicillin, thus providing safe guarantees in the application thereof in the bio-treatment of waste water and remediation of soil environmental pollution.

Description

technical field [0001] The invention belongs to the technical field of biological treatment of environmental pollutants, and in particular relates to a bacterium degrading o-nitrobenzaldehyde and its application in wastewater biological treatment and soil environmental pollution restoration. Background technique [0002] With the acceleration of my country's industrialization process, the discharge of organic industrial synthetic wastewater is increasing. Organic industrial synthetic wastewater refers to industrial wastewater that is toxic to organisms and is discharged during the production process. This type of wastewater not only contains a large amount of organic matter, but also has a large number of substances that are toxic to organisms, which has caused serious pollution to the environment. Threat to human life and health. [0003] O-nitrobenzaldehyde (o-nitrobenzaldehyde, ONBA) is an important fine organic chemical intermediate, widely used in medicine, dyes and or...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34B09C1/10C12R1/05C02F101/38
Inventor 虞方伯周珊管莉菠罗锡平张妙仙单胜道
Owner ZHEJIANG FORESTRY UNIVERSITY
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