Method for preparing polymer microballons of molecular engram of nitrogen benzyl penicillin

An ampicillin and molecular imprinting technology, applied in the field of chemistry and chemical industry, can solve the problems of laborious, time-consuming, low yield, etc., and achieve the effects of high selectivity and sensitivity, high column efficiency, and strong recognition ability

Inactive Publication Date: 2007-12-26
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The polymers mentioned above are mainly bulk polymers obtained by bulk polymerization, and amorphous molecularly imprinted polym...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] This embodiment is implemented under the following conditions of implementation and technical requirements:

[0024] Preparation of Molecularly Imprinted Polymer Microspheres of Ampicillin:

[0025] First prepare a 3% polyvinyl alcohol 1788 solution; then mix DAM, ampicillin, dimethyl sulfoxide, chloroform, EDGMA and AIBN, apply ultrasound for 5 minutes, add it to 100mL polyvinyl alcohol 1788 solution, 60°C, Stir at 400 rpm for 24 h at a constant speed; after the reaction, stir in 70°C water for 60 min, filter, and use ultrasonic oscillation to wash twice with 50 ml of double distilled water, three times with 50 ml of methanol, and twice with 50 ml of acetone (to remove residual dispersion Organic substances such as reagents and monomers), 10min each time, and finally remove the template molecule by methanol: acetic acid (90:10), methanol (100%) Soxhlet extraction; the polymer from which the template molecule has been removed is vacuum-dried (50°C) , to obtain 60 μm pe...

Embodiment 2

[0028] This embodiment is implemented under the following conditions of implementation and technical requirements:

[0029] Preparation of Molecularly Imprinted Polymer Microspheres of Ampicillin:

[0030] First prepare a 6% polyvinyl alcohol 1788 solution; then mix MAA, ampicillin, dimethyl sulfoxide, chloroform, EDGMA and AIBN, apply ultrasound for 10 minutes, add to 100mL polyvinyl alcohol 1788 solution, 80°C, Stir at 600rpm for 24h at a constant speed; after the reaction, stir in 70°C water for 60min, filter, and use ultrasonic oscillation to wash twice with 50ml of double-distilled water, three times with 50ml of methanol, and twice with 50ml of acetone (to remove residual dispersion Organic substances such as reagents and monomers), 10min each time, and finally remove the template molecule by methanol: acetic acid (90:10), methanol (100%) Soxhlet extraction; the polymer from which the template molecule has been removed is vacuum-dried (50°C) , to obtain 15 μm penicillin...

Embodiment 3

[0033] This embodiment is implemented under the following conditions of implementation and technical requirements:

[0034] Preparation of Molecularly Imprinted Polymer Microspheres of Ampicillin:

[0035]First prepare a 4% polyvinyl alcohol 1788 solution; then mix TFMAA, ampicillin, dimethyl sulfoxide, chloroform, EDGMA and AIBN, apply ultrasound for 8 minutes, add to 100mL polyvinyl alcohol 1788 solution, 70°C, Stir at 500 rpm for 24 hours at a constant speed; after the reaction, stir in water at 70°C for 60 minutes, filter, and use ultrasonic oscillation to wash twice with 50ml of double-distilled water, three times with 50ml of methanol, and twice with 50ml of acetone (to remove residual dispersion Organic substances such as reagents and monomers), 10min each time, and finally remove the template molecule by methanol: acetic acid (90:10), methanol (100%) Soxhlet extraction; the polymer from which the template molecule has been removed is vacuum-dried (50°C) , to obtain 40...

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Abstract

This invention discloses a method for preparing ampicillin molecularly imprinted polymer microspheres. The method comprises: preparing 3-6% poly(vinyl alcohol) solution, mixing functional monomers, template molecules, initiator, pore-foaming agent and crosslinker, ultrasonicating, adding into poly(vinyl alcohol) 1788 solution, stirring, stirring in 70 deg.C water for 60 min, filtering, ultrasonicating, washing with double distilled water twice, methanol three times and acetone twice (10 min each time), removing the template molecules, and vacuum-drying at 50 deg.C to obtain ampicillin molecularly imprinted polymer microspheres. The method has such advantages as simple process and high efficiency, and can prepare ampicillin molecularly imprinted polymer microspheres with high molecular recognition ability. When used in solid phase extraction, the ampicillin molecularly imprinted polymer microspheres have such advantages as high column efficiency and high selectivity. The ampicillin molecularly imprinted polymer microspheres can be used for selective separation and high-efficiency enrichment of penicillin in food matrix, such as honey.

Description

technical field [0001] The invention relates to a method in the technical field of chemistry and chemical industry, in particular to a method for preparing ampicillin molecularly imprinted polymer microspheres. Background technique [0002] Ampicillin is a β-lactam semi-synthetic broad-spectrum antibiotic, which was isolated from microbial metabolites for the first time in 1947. Used in veterinary clinics, it is an antibiotic drug commonly used in farming of livestock and poultry and other agricultural products. Ampicillin residues in food are likely to cause allergic reactions to humans, and at the same time can induce the production of drug-resistant strains. When used in combination with tetracycline and chlorpromazine , easy to produce precipitation reaction residues in animal-derived foods. Therefore, penicillin residues in animal-derived foods pose a huge threat to human health. The adverse side effects mainly include mild nausea, vomiting, diarrhea, etc., and may occu...

Claims

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Application Information

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IPC IPC(8): C08J9/26C08F220/10C08F2/44
Inventor 张大兵武爱波史西志
Owner SHANGHAI JIAO TONG UNIV
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