Excised mutagenesis tetraploid method of water melon and ploidy early stage certification technique

A technology of tetraploid and watermelon, applied in the field of in vitro mutagenesis of tetraploid of watermelon, can solve problems such as difficulties, inhibition of adventitious bud regeneration, poor regeneration ability, etc., and achieve the effects of low cost, shortened time, and high induction frequency

Inactive Publication Date: 2005-06-29
刘文革 +1
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Problems solved by technology

Herbaceous plants such as watermelon have poor regenerative ability during in vitro culture, and the use of colchicine in the initial rege

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  • Excised mutagenesis tetraploid method of water melon and ploidy early stage certification technique

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[0018]

[0019] Technical steps of the present invention are as shown in the block diagram above:

[0020] 1. Shoot apex culture: remove the seed coat of the diploid seeds, sterilize with one-thousandth liter of mercury for 10 minutes, wash with sterile water 3 to 5 times, soak the seeds for 8 hours in the dark, and germinate on 1 / 2MS medium. Cultured for 8 days.

[0021] 2. Induction treatment: filter sterilization with dinitroaniline herbicides-Trifluralin (Trifluralin) or Butralin (Butralin) or Pendimethalin (Pendimethalin) at a concentration of 20-200 μmol / L Treat, then add medicinal solution in liquid culture medium (MS+BA2.0mg / L); Diploid watermelon stem tip is cut off, is placed in liquid culture medium (hormone and concentration are BA2.25mg / L) , for a certain period of time in an air-shaking bath at 25°C.

[0022] 3. Differentiation and subculture: Take out the shoot tips in the medicinal liquid, wash them in sterilized water, blot dry with filter paper, culture ...

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Abstract

The invention provides an excised mutagenesis tetraploid method of water melon and ploidy early stage certification technique, wherein dinitro toluene herbicide (DNH) is employed to substitute the conventional colchicines as inducer, whose function is to suppress the Mitosis in the metaphase of cell division through the mechanism of interfering spindle, so as to double the tissue cell chromosome. The method has the advantages of increased inducement success rate and substantially shortened time required for inducement.

Description

technical field [0001] The invention relates to a watermelon in vitro mutagenesis tetraploid method and early ploidy identification technology. Background technique [0002] Triploid seedless watermelons are obtained by crossing tetraploid and diploid watermelons. In order to obtain excellent triploid seedless watermelon varieties, the key point is to obtain tetraploid materials through chromosome doubling of diploid watermelons. Since Kikara reported the research on the breeding of triploid seedless watermelon in 1951, many researchers have successively carried out the research of using colchicine (Colchicine) to induce tetraploid watermelon. There are mainly the following ways to obtain tetraploid watermelon: (1) Excavate natural variation from nature. Some natural phenomena such as lightning, air, drastic temperature changes, chemical substances in the soil, and trauma to watermelon plants can sometimes induce tetraploidy. (2) Induction by physical methods: use various...

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Application Information

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IPC IPC(8): A01H1/04A01H1/08A01H4/00
Inventor 刘文革阎志红
Owner 刘文革
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