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53 results about "Spindle apparatus" patented technology

In cell biology, the spindle apparatus (or mitotic spindle) refers to the cytoskeletal structure of eukaryotic cells that forms during cell division to separate sister chromatids between daughter cells. It is referred to as the mitotic spindle during mitosis, a process that produces genetically identical daughter cells, or the meiotic spindle during meiosis, a process that produces gametes with half the number of chromosomes of the parent cell.

Reagent system and reagent kit for detecting colorectal cancer spindle apparatus assembly checking point and application

The invention relates to a reagent system and a reagent kit for detecting target genes of colorectal cancer spindle apparatus assembly checking point and application thereof. The reagent system comprises a reagent used for detecting BubR1 gene, a reagent used for detecting Mad 1 gene, a reagent for detecting Mad 2 gene, a reagent for detecting AURKB gene, a reagent for detecting PIK 1 gene and a reagent for detecting Cdc20 gene. According to the reagent system and the reagent kit for detecting target genes of colorectal cancer spindle apparatus assembly checking point and the application thereof, specially designed probe and PCR amplification technology are adopted, and through DNA specific binding reaction, the probe and the PCR amplification technology conduct one-time detection on expression state of six genes related to spindle apparatus assembly checking point during a last phase of a mitosis process, thus filling the blank of colorectal cancer detection in genes related to the spindle apparatus assembly checking point. The reagent system and the reagent kit are comprehensive and reliable in detection result, quick and efficient in effect, simple to operate, high in sensitivity, strong in specificity, low in misdiagnosis rate, and is capable of conducting timely precise diagnosis on generation and development of the colorectal cancer in earlier days.
Owner:刘鹏飞

Preparation method of lung cancer stem cell vaccine for obviously improving immunocompetence

The invention provides a preparation method of a lung cancer stem cell vaccine for obviously improving immunocompetence. The method comprises the following steps of (1) culturing the recovered lung cancer stem cells in a DMEM whole culture medium, wherein the DMEM whole culture medium comprises 8 percent of FBS; the culture temperature is 37 DEG C; the humidity is 85 percent; the CO2 concentration is 0.5 percent; the culture time is 3 to 4 days; then, performing 1:3 dish separation on the cultured cell; next, performing culture for 3 days by using the same culture medium under the same culture conditions so that the cells show spindles or are similar to spindles; (2) performing irradiation treatment by 60Co at the dosage of 3 to 4 Gy; (3) selecting CD133 positive stem cells; performing inactivation treatment after the culture; resuspending the stem cells by normal saline so that the cell concentration is 1*10<5> to 1*10<6> /ml; (4) according to the volume ratio of 4:1.5:2, mixing the materials obtained in the step (3), chitosan normal saline solution and laminarin normal saline solution, and after the mixing, the chitosan concentration is 2 percent, and the laminarin concentration is 2.5 percent. The obtained vaccine has the obvious curative effect on the lung cancer.
Owner:CHENGDU YUYA TECH

Automatic ICSI system capable of accurately identifying and operation method

InactiveCN113509250AAvoid damageThe experimental structure is stable and reliableObstetrical instrumentsEmbryoPrenatal development
The invention relates to an automatic ICSI system capable of accurately identifying, which comprises a power supply module, a main control module, a sample optical observation module, a user interaction module, a sperm capturing unit, an ovum fixing unit and a single sperm injection unit, the sample optical observation module, the user interaction module, the sperm capturing unit, the ovum fixing unit and the single sperm injection unit are all electrically connected with the main control module, and the operation method of the system comprises three steps of sperm capturing, ovum fixing and single sperm injection. According to the automatic ICSI system capable of accurately identifying and the operation method, the system can replace manual work to realize automation, is stable and reliable in experimental structure, adopts an AI image system to identify healthy and high-quality sperms and intelligently identify the spindle body part of an ovum, can perform needle insertion and injection on the best-quality sperms through the most appropriate position, greatly reduces the damage to the ovum, and the probability of various fertilization failures or embryo development potential underground caused by human factors is reduced to the greatest extent.
Owner:INLEMS TECH SHENZHEN CO LTD
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