12 results about "Spindle apparatus" patented technology

The invention discloses application of an ACSL4 inhibitor in preparation of a medicine for improving quality reduction of an elderly ovum, and belongs to the technical field of biological medicines. The invention provides an application of targeting long-chain fatty acid acyl-coenzyme A synthetase 4 (ACSL4) in intervention of low oocyte quality and related fertility decline caused by aging. The ACSL4 inhibitor can be used for preparing drugs for improving ovarian functions and improving oocyte quality. In-vivo and in-vitro experiments prove that by inhibiting the ACSL4, the aging process of the elderly ovary can be effectively relieved, the expression of aging-related proteins p16 and p21 can be reduced, the mitochondrial function of the oocyte can be improved, and spindle assembly abnormality and chromosome arrangement defects can be rescued, so that the maturation rate and the development potential of the oocyte can be remarkably improved.

The present invention relates to a method for identifying a compound that modulates the mitotic cell cycle in cancer cells by modulating at least one of the expression, the amount, the stability and / or the biological activity of the protein CITED2 and / or CITED2 containing protein complexes in a mammalian cell. Further provided is a method for determining the level of CITED2 and / or CDK11bp58 and / or splicing factor 3B subunit 1 (SF3B1), and / or 14-3-3γ in a sample obtained from a subject, comprising detecting of binding of CITED2 in the sample to at least one molecule selected from the group consisting of CDK11bp58, splicing factor 3B subunit 1 (SF3B1), and 14-3-3γ, wherein a decrease of the binding, when compared to a control indicates an improper centrosome segregation and subsequent an increase of the formation of chaotic monopolar mitotic spindles in said sample. Further provided are pharmaceutical compositions comprising the compound as identified for use in medicine, in particular for use in the prevention or treatment of a disease or condition caused by cancer.

The present disclosure relates to polynucleotides and methods of inhibiting the activities of development-associated pathways, including the Wnt, Hedgehog, and Notch pathways, and the invasive capacity of malignant cells to treat primary or secondary solid tumors.

We provide a 3D artificial kinetic chorodor scaffold. [Solution] A composition is provided comprising a three-dimensional artificial kinetochore scaffold comprising a unit complex containing NDC80 protein and NUF2 protein. The composition may be biodegradable or proteinaceous, and the number of unit complexes contained in the three-dimensional artificial kinetochore scaffold may be 10,000 to 50,000 or 20,000 to 40,000. The three-dimensional artificial kinetochore scaffold of this disclosure has the ability to align at the spindle equator during meiosis of an oocyte, for example. The three-dimensional artificial kinetochore scaffold of this disclosure can preferably be used, for example, to suppress premature chromosome segregation during meiosis of an oocyte.

The invention discloses a culture solution for improving in-vitro culture blastocyst rate of bovine oocytes and application of the culture solution, and belongs to the technical field of cell in-vitro culture. According to the culture solution, kaempferol is added into a basic culture solution, and the addition concentration of the kaempferol is 0.01-1 mu M / L; preferably, the addition concentration of the kaempferol is 0.1 mu M / L. By optimizing the concentration of the kaempferol and the synergistic proportion of the kaempferol and basic nutritional ingredients, the action mechanism of the kaempferol for regulating and controlling the mitochondrial function of the oocyte and meiosis spindle assembly is defined, and the direct association of'oxidation resistance-development potential improvement 'is established, so that the blastocyst formation rate and embryo quality of in-vitro culture of the oocyte are remarkably improved, and the development potential of the oocyte is improved. The requirements of the fields of assisted reproduction, embryo engineering and the like on an efficient and stable oocyte in-vitro culture technology are met.

The application belongs to the technical field of cell biology, and provides an oocyte in vitro maturation culture medium additive and application thereof. The experimental results of the application show that coumarin 106 can improve the meiosis process blockage of oocytes, reduce the morphological proportion of abnormal spindle and the proportion of misaligned chromosomes, restore the level of acetylated tubulin, improve microtubule stability, improve the expression level of actin in the spindle of oocytes, improve the misdistribution and expression decrease of mitochondria in oocytes, improve the level of mitochondrial membrane potential, improve the fluorescence signal of cortical granules and specific cortical granule proteases in oocytes, improve the fertilization rate, reduce the level of reactive oxygen species and DNA damage in oocytes, and reduce the occurrence of early apoptosis in oocytes. The culture medium prepared by using coumarin 106 can be used for promoting oocyte in vitro maturation.

This invention relates to the field of reproductive engineering technology, specifically to the application of rutabagastone in improving the cryopreservation and in vitro maturation of oocytes. This invention is the first to clearly demonstrate that rutabagastone can improve the quality of cryopreservation and in vitro maturation of frozen oocytes. By adding rutabagastone to the cryopreservation solution, thawing solution, and in vitro maturation solution, this invention reduces cryopreservation damage to oocytes, resulting in increased survival rate after thawing, improved mitochondrial function, reduced damage to the oocyte spindle and zona pellucida, reduced oxidative stress levels, and ultimately improved subsequent developmental capacity of frozen oocytes.

The invention discloses a novel application of a compound beta-alanine (beta-alanine) in preparation of a medicine for improving oocyte quality and treating ovarian endometriosis (OEM) related infertility, and belongs to the technical field of biological medicines. An OEM animal model proves that beta-alanine can remarkably improve reproductive function disorder of model animals, specifically, follicular dysplasia caused by endometriosis is effectively relieved, the fertility is improved, and the litter size is increased; in the aspect of oocyte quality, the compound can promote normal discharge of a first polar body in an oocyte in-vitro maturation process, reduce cytoplasm fragmentation, recover spindle body form and microtubule arrangement, enhance mitochondrial membrane potential stability and maintain the integrity of an ultrastructure. The results show that beta-alanine can reverse oocyte quality reduction caused by ovarian endometriosis in multiple dimensions, has a definite treatment potential, and provides an important candidate molecule for developing a novel assisted reproduction intervention strategy for targeting oocyte health.

The invention provides application of endothelin in oocyte in-vitro maturation and embryo in-vitro culture. New application of endothelin is found, the endothelin can improve the polar body discharge rate of oocytes matured in vitro, promote cytoplasm maturing and improve the normal arrangement proportion of organelles after maturing, certain promotion is provided for improvement of oocyte fertilization, and meanwhile subsequent blastocyst development is promoted. The invention further provides an in-vitro maturation culture solution of the sheep oocytes and an in-vitro maturation method of the sheep oocytes, the in-vitro maturation culture solution contains endothelin, and after the sheep oocytes are treated with the in-vitro maturation culture solution containing the endothelin concentration, the mitochondrial membrane potential level of the oocytes can be remarkably improved, and the survival rate of the sheep oocytes is increased. ROS (reactive oxygen species) in the oocytes is remarkably reduced, and the ATP (adenosine triphosphate) and GSH (glutathione) levels of the oocytes and the normal proportion level of spindles are improved; the maturation rate, the fertilization efficiency, the blastocyst number and the blastocyst rate are remarkably increased, in-vitro maturation of sheep oocytes and in-vitro development of early embryos are promoted, and the production efficiency of the sheep in-vitro embryos is effectively improved.

The application discloses a culture medium for improving in-vitro aging of oocytes after ovulation and application thereof. The culture medium is prepared by adding 0.1-30 muM of astragaloside IV into an oocyte culture solution. The culture medium is safe, non-toxic and free of side effects, can reduce the oocyte fragmentation rate, active oxygen level and abnormal spindle rate of the oocytes after 24 hours of in-vitro culture, can significantly improve the mitochondrial membrane potential, in-vitro sperm binding capacity, in-vitro fertilization pronucleus rate and cleavage rate of the mature oocytes after 24 hours of in-vitro culture, and can improve the quality of the late embryos. The application of the culture medium provides favorable support for assisted reproductive technology, and has a good application prospect.

The invention belongs to the field of reproductive medicine, and particularly relates to application of deaminotyrosine in preparation of a reagent or a medicine for improving the quality of aged oocytes. The invention discloses and provides a mechanism for activating an ERK signal channel by utilizing deaminotyrosine specificity to act on the quality improvement of the aged oocytes for the first time. Experiments prove that the deaminotyrosine can reduce the abnormal form proportion of spindle bodies of oocytes, regulate the transformation of mitochondria to uniform distribution, improve the membrane potential of the mitochondria, reduce the ROS level, improve the maturation rate of the oocytes and improve the development rate of in-vitro fertilized blastocysts. The deaminated tyrosine is an endogenous and symbiotic microbial metabolite of a human body, so that the deaminated tyrosine has extremely high biological safety while improving the quality of aged oocytes, and an effective and safe new means is provided for improving the fertility potential of elderly women and optimizing and assisting a reproductive in-vitro maturation technology.

The invention belongs to the technical field of biology, and particularly relates to a preparation for in-vitro embryo production, a culture medium and application thereof, and the preparation contains the following components: an Ephrin ligand family. The preparation for in-vitro embryo production contains the Ephrin ligand family and can effectively improve the in-vitro maturation quality and development efficiency of oocytes, and further, a flavonoid compound can be added on the basis, so that the preparation can be used for improving the in-vitro maturation quality and development efficiency of the oocytes through the synergistic effect of the Ephrin ligand family and the flavonoid compound. The invention has the advantages that the effects of enhancing mitochondrial function and ATP (adenosine triphosphate) synthesis capability, removing reactive oxygen species (ROS), reducing DNA (deoxyribonucleic acid) damage and spindle abnormality rate, inhibiting proinflammatory factor expression and NF [kappa] B signal channel activity and the like are exerted, so that the in-vitro maturation quality and embryonic development potential of the oocyte are obviously improved, and the application prospect is wide.