39 results about "Calcifediol" patented technology

The invention belongs to the technical field of biomedicine, and in particular relates to a vitamin D supplement preparation and application thereof. The active ingredients of the vitamin D supplement preparation provided by the invention include: calcifediol and oleanolic acid. The synergistic effect of calcifediol and oleanolic acid can effectively promote the expression of CYP27B1 in bone marrow stem cells and osteoblasts, and increase the level of 1,25‑dihydroxyvitamin D 3 Synthesis of vitamin D, which can quickly supplement the vitamin D needed by the human body, especially in special populations 3 , regulate calcium and phosphorus metabolism, promote bone mineral deposition, and can be applied to the preparation of prevention or treatment of bone diseases caused by vitamin D deficiency.

The present invention relates to a buffer composition for promoting production of calcitriol or calcifediol, and a method for producing calcitriol or calcifediol using the same. More particularly, the present invention relates to a buffer composition for promoting production of calcitriol or calcifediol comprising a metallic compound, an organic solvent, cyclodextrin, tris(hydroxymethyl)aminomethane, sodium succinate, sodium chloride, magnesium chloride, and water, and a method for producing calcitriol or calcifediol using the same. In the method for producing calcitiriol or calcifediol, the production yield of calcitriol or calcifediol is high, and the bioconversion is carried out in an enzyme reaction system instead of in a microorganism culture system. Thus, it is not required to maintain a sterile state. Also, the separation / purification following the completion of a biocatalytic reaction can be carried out in a cleaner state than the microorganism culture method. Accordingly, there is an advantage in that a cost required for separation is low and the quality is improved. Furthermore, the buffer composition for promoting production of calcitriol or calcifediol can provide a high productivity of calcitriol or calcifediol.

The use of 25-OH D3 (calcifediol) to increase muscle strength, muscle function, or both is provided. Vitamin D3 (cholecalciferol) may optionally be used together with 25-OH D3. Forms and dosages of a pharmaceutical composition, as well as processes for manufacturing medicaments, are also disclosed.

The invention discloses a feeding medium for calcifediol fermentation, a feeding method for fermentation and a fermentation method thereof. The feeding medium includes: organic nitrogen source 0.2-3.0 g / 100ml, inorganic nitrogen source 0.2-3.0 g / 100ml, carbon source 0.5~3.0g / 100ml, inorganic salt A 0.1~1.0g / 100ml, inorganic salt B 0.1~1.0g / 100ml, trace element 0.0002~0.0006g / 100ml. The feeding method and the fermentation method include adding the feeding medium and water to the fermentation broth, which meets the nutritional requirements of calcifediol fermentation and satisfies the dissolved oxygen requirements of calcifediol fermentation, The power cost is reduced and the fermentation efficiency is improved.

A medicine composition contains vitamin D and metformin, wherein vitamin D comprises vitamin D2, vitamin D3, alphacalcidol, calcifediol, calcitriol, and dihydrotachysterol. The composition can be used in the preparation of a medicine for treating and / or preventing a polyp and cancer in large intestine.

A novel calcifediol (25-hydroxyvitamin D3) separation and purification method is provided. The method mainly includes subjecting calcifediol to extraction and separation with an organic solvent; concentrating an extract liquid; dissolving the concentrate again to obtain a calcifediol stock solution for column chromatography; preliminarily purifying the calcifediol product by utilizing a gel chromatographic column; then further purifying and separating the calcifediol product by utilizing macroporous adsorption resin; finally preparing a high-purity calcifediol product through column chromatography on silica gel; subjecting an eluate to concentration and crystallization to obtain a calcifediol crystal product. The method is advantageous in that 1) the calcifediol product obtained by separation and purification through the method is high in purity and high in yield; 2) gel column chromatography and macroporous resin adsorption chromatography are applied for separation and purification ofthe calcifediol product for the first time; 3) the method is simple in process and convenient to operate, a plurality of times of cyclic operation can be performed, the equipment cost is low and themethod is particularly suitable for industrial promotion; 4) organic solvents adopted in the method can be recycled and reused, so that the production cost is reduced, and the method is economical andenvironmentally friendly.