Feeding medium for calcifediol fermentation, feeding method for fermentation and fermentation method thereof

A technology of feeding medium and calcifediol, which is applied in the field of feeding medium for calcifediol fermentation, can solve the problems of low fermentation efficiency, large evaporation of fermentation liquid, increased viscosity of fermentation liquid, etc. Microbial environment, the effect of improving fermentation efficiency and reducing fermentation viscosity

Active Publication Date: 2021-08-03
太原市威尔潞威科技发展有限公司 +1
View PDF8 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are also some problems in the implementation of microbial fermentation. In the fermentation process, the fermentation time is long, and the supply of nutrients may be insufficient. The viscosity of the fermentation broth increases, the oxygen transfer is limited, the mycelium breaks and autolyzes prematurely, and the pH value of the fermentation broth rises, which will affect the activity of the enzyme in the mycelium, and the result is the substrate Vitamin D 3 There are many residues, the output of the product calcifediol increases slowly, and the fermentation time is long, resulting in low fermentation efficiency

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Feeding medium for calcifediol fermentation, feeding method for fermentation and fermentation method thereof
  • Feeding medium for calcifediol fermentation, feeding method for fermentation and fermentation method thereof
  • Feeding medium for calcifediol fermentation, feeding method for fermentation and fermentation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0071] 1)

[0072] A) Inclined bacteria:

[0073] The slant medium is composed of glucose 1.5g / l00ml, peptone 1.0g / l00ml, corn steep liquor 0.3g / l00ml, sodium chloride 0.4g / l00ml, agar 1.5g / l00ml and the rest of water; the pH value of the slant medium is 7.0 .

[0074] The bacterial strain with the preservation number CGMCC No.7935 was inoculated into the slant medium for culture to obtain the strain with the preservation number CGMCC No.7935.

[0075] B) Seed solution:

[0076] The seed medium consists of peptone 1.5g / l00ml, corn steep liquor 1.5g / l00ml, glucose 1.5g / l00ml, soybean powder 0.4g / l00ml, sodium chloride 0.5g / l00ml, potassium dihydrogen phosphate 0.2g / l00ml and the rest Water composition; the pH value of the seed medium is 7.5.

[0077] Inoculate the strain with the preservation number CGMCC No.7935 into 100ml of seed culture medium and 500ml Erlenmeyer flask, shake the flask at a speed of 280r / min, and culture at 29°C for 3 days to obtain a first-class seed l...

Embodiment 2

[0085] The difference between this example and Example 1 is that the feed medium includes: organic nitrogen source 0.5g / l00ml, inorganic nitrogen source 0.2g / l00ml, carbon source 0.5g / l00ml, inorganic salt A 0.2g / l00ml, Inorganic salt B 0.2g / l00ml, trace elements 0.0003g / l00ml; the organic nitrogen source is peptone, the inorganic nitrogen source is ammonium sulfate, the carbon source is glucose, and the inorganic salt A is sodium dihydrogen phosphate, The inorganic salt B is magnesium sulfate, and the trace elements are manganese chloride and ferric chloride.

[0086] Test results: the fermentation time in the step 3) was 10 days, and the HPLC analysis of the liquid obtained showed that the content of calcifediol was 0.75 mg / ml.

Embodiment 3～7

[0088] The difference between this example and Example 1 is only that the organic nitrogen source, the inorganic nitrogen source, the carbon source, the inorganic salt A, the inorganic salt B, the trace element And the selection of the amino acid, the selection of the feed medium components and the test results: the fermentation time in the step 3) and the HPLC analysis of the calcifediol content of the feed liquid are shown in Table 1.

[0089] Table 1

[0090]

[0091]

[0092] From Examples 1 to 2, 3 to 7, it can be seen that in the feed medium, preferably, the organic nitrogen source is selected from one or more of peptone, soybean powder, peanut powder, corn steep liquor and yeast extract, and more preferably Preferably, the organic nitrogen source is peptone; preferably, the inorganic nitrogen source is selected from one or more of ammonium sulfate, ammonium nitrate, urea and ammonia, more preferably, the inorganic nitrogen source is sulfuric acid Ammonium; prefer...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a feeding medium for calcifediol fermentation, a feeding method for fermentation and a fermentation method thereof. The feeding medium includes: organic nitrogen source 0.2-3.0 g / 100ml, inorganic nitrogen source 0.2-3.0 g / 100ml, carbon source 0.5~3.0g / 100ml, inorganic salt A 0.1~1.0g / 100ml, inorganic salt B 0.1~1.0g / 100ml, trace element 0.0002~0.0006g / 100ml. The feeding method and the fermentation method include adding the feeding medium and water to the fermentation broth, which meets the nutritional requirements of calcifediol fermentation and satisfies the dissolved oxygen requirements of calcifediol fermentation, The power cost is reduced and the fermentation efficiency is improved.

Description

technical field [0001] The invention relates to the field of biological technology, in particular to a feeding medium for calcifediol fermentation, a feeding method for fermentation and a fermentation method thereof. Background technique [0002] Calcifediol (25-hydroxyvitamin D) in vitamin D drugs 3 ) is vitamin D that occurs naturally in the human body 3 The active metabolites are widely used. It is mainly used to treat various chronic bone disorders such as osteoporosis in the elderly, as well as metabolic bone diseases related to chronic renal failure, hypocalcemia. In addition, calcifediol has also been used in the feed industry as a feed additive in recent years. [0003] There are two methods for the preparation of calcifediol: chemical synthesis and biological fermentation; chemical synthesis has many steps, requires expensive reagents, and racemates will be produced during the reaction process, which requires complex resolution processes, which not only greatly a...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/02C12N1/20C12R1/01
CPCC12N1/20C12P7/02
Inventor 梁彦明曾志刚翟龙飞许之晖
Owner 太原市威尔潞威科技发展有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap