Vitamin D supplementing preparation and application thereof

A technology of vitamins and preparations, applied in the field of biomedicine, to reduce dosage, regulate calcium and phosphorus metabolism, and promote obvious effects

Active Publication Date: 2021-02-23
THE HONG KONG POLYTECHNIC UNIV SHENZHEN RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, due to the decline of body function or the antagonistic effect of taking drugs, the improvement of vitamin D deficiency in some special populations cannot directly benefit from the supplementation of cholecalciferol and ergocalciferol

Method used

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  • Vitamin D supplementing preparation and application thereof
  • Vitamin D supplementing preparation and application thereof
  • Vitamin D supplementing preparation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] This example studies the effect of oleanolic acid (oleanolic acid, OA) on the expression activity of CYP27B1 in osteoblasts, and CYP27B1 catalyzes 25OHD 3 Metabolized to 1,25(OH)2D 3 .

[0047] 1. Select the MG-63 human osteoblast-like cell line, and randomly divide it into several test groups: blank group, the concentration is 10 -7 M's parathyroid hormone (PTH) group, the concentration is 10 -6 M25OHD 3 group and concentration were 10 -9 、10 -8 、10 -7 、10 -6 、10 -5 In the oleanolic acid group of M, no substrate was added to the blank group; after each test group was incubated with MG-63 cells for 24 hours, the expression level of CYP27B1 enzyme in osteocytes of each composition was detected, and β-Actin was used as an internal reference protein.

[0048] Such as figure 1 The results showed that oleanolic acid at a higher concentration (10 -6 -10 -5 M) and lower concentrations (10 -9 -10 -8 M) can significantly improve the expression level of CYP27B1 enzym...

Embodiment 2

[0052] This example studies the effect of oleanolic acid (oleanolic acid, OA) on the expression activity of CYP27B1 in human bone marrow mesenchymal stem cells.

[0053] 1. Human bone marrow mesenchymal stem cells were selected and randomly divided into multiple test groups: blank group, concentration 10 -7 The parathyroid hormone (PTH) group and concentration of M were 10 -9 、10 -8 、10 -7 、10 -6 In the oleanolic acid group, the blank group did not add any substrate; after the human bone marrow mesenchymal stem cells were incubated for 24 hours in each test group, the expression level of CYP27B1 in each group was detected, and β-Actin was used as an internal reference protein.

[0054] Such as image 3 The results showed that different concentrations of oleanolic acid could increase the expression level of CYP27B1 in human bone marrow mesenchymal stem cells to varying degrees, and the concentration was 10 -9 The oleanolic acid of M has statistical difference (p-8 M's olea...

Embodiment 3

[0058] In this example, the concentration of 10 -7 M of calcifediol with a concentration of 10 -9 -10 -7 The effect of M's oleanolic acid combination on the expression and activity of CYP27B1 in osteoblasts.

[0059] 1. Select the MG-63 human osteoblast-like cell line and randomly divide it into multiple test groups: blank group, calcifediol (25OHD 3 ) alone medication group and OA and calcifediol combined medication group; wherein, in the test group, the final concentration of calcifediol was 10 -7 The final concentrations of M and OA were 10 -9 、10 -8 、10 -7 、10 -6 、10 -5 M, no substrate was added to the blank group; then, each test group was incubated with MG-63 cells for 24 hours, and the expression level of CYP27B1 in each bone cell was detected, and β-Actin was used as an internal reference protein.

[0060] Such as Figure 5 The results showed that OA(10 -8 M) and 25OHD 3 (10 -7 M) Combined medication group relative to 25OHD 3 (10 -7 M) There is a statisti...

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Abstract

The invention belongs to the technical field of biological medicine, and particularly relates to a vitamin D supplementing preparation and application thereof. The vitamin D supplementing preparationcomprises the following active ingredients: calcifediol and oleanolic acid. The calcitriol and the oleanolic acid have a synergistic effect, so that bone marrow stem cells and osteoblasts can be effectively promoted to express CYP27B1, the synthesis of 1,25-dihydroxy vitamin D3 is improved, vitamin D3 required by human bodies, particularly special crowds, can be quickly supplemented, calcium and phosphorus metabolism can be regulated, bone mineral deposition can be promoted, and the vitamin D supplementing preparation can be applied to preparation of medicines for preventing or treating bone diseases caused by vitamin D deficiency.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a vitamin D supplement preparation and application thereof. Background technique [0002] Vitamin D is a fat-soluble vitamin necessary for the human body and belongs to steroid derivatives. Among them, vitamin D is closely related to human health 2 and D 3 , which are derivatives of different provitamin D after ultraviolet irradiation. Vitamin D 2 or D 3 None of them are biologically active, and their metabolic pathways include: after being synthesized in the skin or absorbed from the gastrointestinal tract, they enter various tissues through the blood. Bioactive 25-Hydroxyvitamin D 3 , and then further metabolized into more active 1,25-dihydroxyvitamin D under the catalysis of 25-hydroxyvitamin D-1α-hydroxylase (CYP27B1) encoded by the CYP27B1 gene present in other tissues 3 , after which, 25-hydroxyvitamin D encoded in the CYP24A1 gene 3 -24-hydroxylase (...

Claims

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Application Information

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IPC IPC(8): A61K31/593A61K31/56A61P3/02A61P19/10A61P19/08A61P5/16A61P13/12A23L33/155
CPCA61K31/593A61K31/56A61P3/02A61P19/10A61P19/08A61P5/16A61P13/12A23L33/155A23V2002/00A23V2200/30A23V2200/306A23V2250/71A61K2300/00
Inventor 黄文秀董晓莉于文轩
Owner THE HONG KONG POLYTECHNIC UNIV SHENZHEN RES INST
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