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Feeding culture medium by fermenting calcifediol, feeding method for fermentation and fermentation method of calcifediol

A technology of feeding medium and calcifediol, which is applied in the field of feeding medium for calcifediol fermentation, can solve the problem of slow growth of product calcifediol production, affecting enzyme activity in mycelium and pH value of fermentation broth. It can improve the oxygen transfer process, avoid volume reduction, and meet the needs of dissolved oxygen.

Active Publication Date: 2017-11-21
太原市威尔潞威科技发展有限公司 +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are also some problems in the implementation of microbial fermentation. In the fermentation process, the fermentation time is long, and the supply of nutrients may be insufficient. The viscosity of the fermentation broth increases, the oxygen transfer is limited, the mycelium breaks and autolyzes prematurely, and the pH value of the fermentation broth rises, which will affect the activity of the enzyme in the mycelium, and the result is the substrate Vitamin D 3 There are many residues, the output of the product calcifediol increases slowly, and the fermentation time is long, resulting in low fermentation efficiency

Method used

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  • Feeding culture medium by fermenting calcifediol, feeding method for fermentation and fermentation method of calcifediol
  • Feeding culture medium by fermenting calcifediol, feeding method for fermentation and fermentation method of calcifediol
  • Feeding culture medium by fermenting calcifediol, feeding method for fermentation and fermentation method of calcifediol

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0071] 1)

[0072] A) Inclined bacteria:

[0073] The slant medium is composed of glucose 1.5g / l00ml, peptone 1.0g / l00ml, corn steep liquor 0.3g / l00ml, sodium chloride 0.4g / l00ml, agar 1.5g / l00ml and the rest of water; the pH value of the slant medium is 7.0 .

[0074] The bacterial strain with the preservation number CGMCC No.7935 was inoculated into the slant medium for culture to obtain the strain with the preservation number CGMCC No.7935.

[0075] B) Seed solution:

[0076] The seed medium consists of peptone 1.5g / l00ml, corn steep liquor 1.5g / l00ml, glucose 1.5g / l00ml, soybean powder 0.4g / l00ml, sodium chloride 0.5g / l00ml, potassium dihydrogen phosphate 0.2g / l00ml and the rest Water composition; the pH value of the seed medium is 7.5.

[0077] Inoculate the strain with the preservation number CGMCC No.7935 into 100ml of seed culture medium and 500ml Erlenmeyer flask, shake the flask at a speed of 280r / min, and culture at 29°C for 3 days to obtain a first-class seed l...

Embodiment 2

[0085] The difference between this embodiment and Example 1 is that the feed medium includes: organic nitrogen source 0.5g / l00ml, inorganic nitrogen source 0.2g / l00ml, carbon source 0.5g / l00ml, inorganic salt A0.2g / l00ml, Inorganic salt B 0.2g / l00ml, trace elements 0.0003g / l00ml; the organic nitrogen source is peptone, the inorganic nitrogen source is ammonium sulfate, the carbon source is glucose, and the inorganic salt A is sodium dihydrogen phosphate, The inorganic salt B is magnesium sulfate, and the trace elements are manganese chloride and ferric chloride.

[0086] Test results: the fermentation time in the step 3) was 10 days, and the HPLC analysis of the liquid obtained showed that the content of calcifediol was 0.75 g / ml.

Embodiment 3～7

[0088] The difference between this example and Example 1 is only that the organic nitrogen source, the inorganic nitrogen source, the carbon source, the inorganic salt A, the inorganic salt B, the trace element And the selection of the amino acid, the selection of the feed medium components and the test results: the fermentation time in the step 3) and the HPLC analysis of the calcifediol content of the feed liquid are shown in Table 1.

[0089] Table 1

[0090]

[0091]

[0092] From Examples 1 to 2, 3 to 7, it can be seen that in the feed medium, preferably, the organic nitrogen source is selected from one or more of peptone, soybean powder, peanut powder, corn steep liquor and yeast extract, and more preferably Preferably, the organic nitrogen source is peptone; preferably, the inorganic nitrogen source is selected from one or more of ammonium sulfate, ammonium nitrate, urea and ammonia, more preferably, the inorganic nitrogen source is sulfuric acid Ammonium; prefer...

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Abstract

The invention discloses a feeding culture medium by fermenting calcifediol, a feeding method for fermentation and a fermentation method of the calcifediol. The feeding culture medium comprises, by concentration, 0.2-3.0g / 100ml of an organic nitrogen source, 0.2-3.0g / 100ml of an inorganic nitrogen source, 0.5-3.0g / 100ml of a carbon source, 0.1-1.0g / 100ml of inorganic salt A, 0.1-1.0g / 100ml of inorganic salt B and 0.0002-0.0006g / 100ml of a microelement. The feeding method and the fermentation method comprise the steps that the feeding culture medium and water are added into fermentation broth, therefore the requirements for nutrient ingredients of fermentation of the calcifediol are satisfied, the requirements for oxygen dissolution of fermentation of the calcifediol are satisfied, the power cost is lowered, and the fermentation efficiency is improved.

Description

technical field [0001] The invention relates to the field of biological technology, in particular to a feeding medium for calcifediol fermentation, a feeding method for fermentation and a fermentation method thereof Background technique [0002] Calcifediol (25-hydroxyvitamin D) in vitamin D drugs 3 ) is vitamin D that occurs naturally in the human body 3 The active metabolites are widely used. It is mainly used to treat various chronic bone disorders such as osteoporosis in the elderly, as well as metabolic bone diseases related to chronic renal failure, hypocalcemia. In addition, calcifediol has also been used in the feed industry as a feed additive in recent years. [0003] There are two methods for the preparation of calcifediol: chemical synthesis and biological fermentation; chemical synthesis has many steps, requires expensive reagents, and racemates will be produced during the reaction process, which requires complex resolution processes, which not only greatly af...

Claims

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Application Information

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IPC IPC(8): C12P7/02C12N1/20C12R1/01
CPCC12N1/20C12P7/02
Inventor 梁彦明曾志刚翟龙飞许之晖
Owner 太原市威尔潞威科技发展有限公司
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