Preparation method of vinegar-egg powder, compound containing vinegar-egg powder and application thereof
A technology of vinegar egg powder and composition, which is applied in the field of vinegar egg powder, can solve the problems of palliative treatment, not the root cause, difficulty in absorption, etc., and achieve the effect of non-toxic side effects and increasing bone density
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Embodiment 1
[0029] Embodiment 1: the preparation method of fruit vinegar egg powder
[0030]Get 2500g of oranges, wash them with clear water, peel them, and process them into 1500g of fruit juice with a crusher and a beater (0.5mm aperture). Add 0.1% food-grade pectinase for clarification, the temperature is 40°C, and the natural pH value is about 4. After reacting for 2 hours, filter with a plate and frame filter press (filter cloth 120 mesh), and then heat at 80°C for 15 minutes to extinguish bacteria. Cool the citrus juice to 28°C, add activated yeast, ferment at 28°C for three days, stop the fermentation when the alcohol content of the fermentation broth is 6-7% (v / v), and the total sugar drops below 10g / l. Get citrus cider. Add 3% of the cultivated acetic acid bacteria, the stirring speed is 150r / min, the ventilation ratio is 1:0.8, ferment at 32°C for 4 days, and stop the fermentation when the total acid (in terms of acetic acid) rises to 6-7%. After removing thalline by pressure...
Embodiment 6
[0038] Embodiment 6: safety toxicology test
[0039] 1 Materials and methods
[0040] 1.1 Test substance: the tablet produced according to the formula of Example 1 of the present invention, hereinafter referred to as the test substance.
[0041] 1.2 Experimental animals: (Experiments 2 and 3 below) ICR mice and SD rats were provided by Zhejiang Experimental Animal Center, medical experimental animal certificate number 22001001, clean grade. (4 Feeding experiment) SD rats were provided by the Shanghai Experimental Animal Center of the Chinese Academy of Sciences, and the qualification certificate number is Zhongke Dongguan No. 003, clean grade, body weight 60-80g.
[0042] 2 Acute toxicity test
[0043] SD rats weigh 180-220g, 20 males and 20 each; ICR mice weigh 18-22g, 20 males and 20 males. SD rats and ICR mice were randomly divided into four dose groups of 1.00g / kg, 2.15g / kg, 4.64g / kg, and 10.00g / kg body weight according to Horn's method, with 5 male and 5 rats in each g...
Embodiment 7
[0071] Embodiment 7: increase bone density test
[0072] In order to verify the efficacy of the composition of the present invention, the Chinese Center for Disease Control and Prevention was entrusted to complete the animal efficacy test.
[0073] 1 Materials and methods
[0074] 1.1 Sample: The tablet produced according to the formula of Example 1 of the present invention, hereinafter referred to as the test substance, is provided by Hangzhou Xinfu Pharmaceutical Co., Ltd. The recommended intake is 4 tablets per person each time, once a day.
[0075] 1.2 Experimental animals: 50 clean-grade weaned WISTAR male rats with an initial weight of 60-75 g, provided by the Institute of Experimental Animals, Chinese Academy of Medical Sciences, license number: SCXK (Beijing) 2000-0006.
[0076] 1.3 Test method: Divide the rats into 5 groups at random, namely the low-calcium feed control group, the low-, medium-, and high-dose test groups of the test substance, which are respectively ...
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