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Method for promoting plant seed germination under cadmium stress

A technology for plant seeds and seeds, applied in the fields of botanical equipment and methods, plant growth regulators, seed and rhizome treatment, etc., can solve problems such as inapplicability, improve germination rate, alleviate oxidative stress, and reduce membrane lipid peroxidation. horizontal effect

Inactive Publication Date: 2015-06-24
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the usual pretreatment method is to use a variety of substances mixed according to a specific formula, and most of these formulas have obvious effects on one or a few plant seeds, and there is a certain species specificity.
In addition, some researchers put hydrogen peroxide (H 2 o 2 ), nitric oxide (NO), jasmonic acid (JA), salicylic acid (SA) and other signal substances that play a role in the process of plant stress resistance are used in the research of plant seed resistance to cadmium, but this method also exists only Problems that are valid for a few specific species, cannot be applied to crops such as cucumber, switchgrass, etc.

Method used

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  • Method for promoting plant seed germination under cadmium stress
  • Method for promoting plant seed germination under cadmium stress
  • Method for promoting plant seed germination under cadmium stress

Examples

Experimental program
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Effect test

Embodiment 1

[0045]300 cucumber seeds (Jinyan No. 4) were pretreated by soaking in 100ml of melatonin with a concentration of 0, 10, 50, 100, and 250 μmol / L for 12 hours at 25°C in the dark, respectively denoted as CK , M10, M50, M100, M250. Then wash with distilled water for 3 times and air-dry in the shade to the weight before soaking. The seeds were placed in germination boxes with 2 layers of filter paper to germinate in the dark at 25°C, 50 seeds per box, and 4 replicates. Add 10ml of 2mmol / L cadmium chloride solution to the germination box for cadmium stress treatment. After 12 hours, start to count the number of germinations, and count every hour thereafter until the germination is complete. Such as figure 1 As shown, the germination rate of cucumber seeds pretreated with melatonin was significantly higher than that of the control. At 17h, the germination rate of M100 was 43% higher than that of the control.

Embodiment 2

[0047] 300 grains of switchgrass seeds (Alamo) were pretreated by soaking in 50ml of melatonin with a concentration of 0, 10, 50, and 250 μmol / L for 18 hours at 25°C in the dark, respectively CK, M10, M50, M250 . Then wash with distilled water for 3 times and air-dry in the shade to the weight before soaking. The seeds were placed in germination boxes with 2 layers of filter paper to germinate in the dark at 25°C, 50 seeds per box, and 3 replicates. Add 10ml of 5mmol / L cadmium chloride solution to the germination box. After 3 days, start counting the number of sprouts, and count once a day thereafter until the germination is complete. Such as figure 2 , the germination rate of switchgrass seeds pretreated with melatonin was significantly higher than that of the control.

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Abstract

The invention relates to a method for promoting plant seed germination under cadmium stress. The method comprises the treatment of plant seeds through melatonin before germination. The method comprises the following step: enabling the plant seeds to contact with 10 to 250 micron mol / L by concentration of melatonin. With the adoption of the method, the germination potential of the plant seeds, in particular cucumber seeds and switchgrass seeds under the cadmium stress, can be effectively increased; the germination rate can be increased; the seeds can rapidly germinate uniformly; the important topic on the way of enabling normal germination of the seeds in the soil polluted by cadmium in the agricultural production is solved; the method has an important application value in production; melatonin is utilized to pre-treat, so that the expression of antioxidant enzyme activities can be induced, a large amount of free radicals produced under the cadmium stress can be removed, the oxidation stress is relieved, the oxidation damage is reduced, the seeds can be kept in a relatively small membrane lipid peroxidation level, and as a result, the seeds can germinate normally.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for promoting seed germination under cadmium stress. Background technique [0002] Heavy metal pollution generally refers to the pollution caused by metals or their compounds with a specific gravity above 4 (mainly including copper, zinc, chromium, lead, mercury, nickel, cobalt, etc.) in the environment. Among all kinds of heavy metal pollution, cadmium pollution is the most widespread, and various cadmium pollution incidents have emerged in an endless stream in recent years. The toxicity of cadmium is very high, and the main source is the wastewater discharged from electroplating, mining, smelting, fuel, battery and chemical industries. Growth retardation, reduced dry matter production, etc. [0003] The life of a plant begins with seeds, and seed germination is restricted by external environmental conditions, including light, temperature, water, oxygen, etc. In field pr...

Claims

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Application Information

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IPC IPC(8): A01C1/00A01N43/38A01P21/00
Inventor 郭仰东曹芸运郑禾辛燕李海东张娜齐传东孙倩倩连蔚然徐凤凤
Owner CHINA AGRI UNIV
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