Method for promoting plant seed germination under cadmium stress
A technology for plant seeds and seeds, applied in the fields of botanical equipment and methods, plant growth regulators, seed and rhizome treatment, etc., can solve problems such as inapplicability, improve germination rate, alleviate oxidative stress, and reduce membrane lipid peroxidation. horizontal effect
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Embodiment 1
[0045]300 cucumber seeds (Jinyan No. 4) were pretreated by soaking in 100ml of melatonin with a concentration of 0, 10, 50, 100, and 250 μmol / L for 12 hours at 25°C in the dark, respectively denoted as CK , M10, M50, M100, M250. Then wash with distilled water for 3 times and air-dry in the shade to the weight before soaking. The seeds were placed in germination boxes with 2 layers of filter paper to germinate in the dark at 25°C, 50 seeds per box, and 4 replicates. Add 10ml of 2mmol / L cadmium chloride solution to the germination box for cadmium stress treatment. After 12 hours, start to count the number of germinations, and count every hour thereafter until the germination is complete. Such as figure 1 As shown, the germination rate of cucumber seeds pretreated with melatonin was significantly higher than that of the control. At 17h, the germination rate of M100 was 43% higher than that of the control.
Embodiment 2
[0047] 300 grains of switchgrass seeds (Alamo) were pretreated by soaking in 50ml of melatonin with a concentration of 0, 10, 50, and 250 μmol / L for 18 hours at 25°C in the dark, respectively CK, M10, M50, M250 . Then wash with distilled water for 3 times and air-dry in the shade to the weight before soaking. The seeds were placed in germination boxes with 2 layers of filter paper to germinate in the dark at 25°C, 50 seeds per box, and 3 replicates. Add 10ml of 5mmol / L cadmium chloride solution to the germination box. After 3 days, start counting the number of sprouts, and count once a day thereafter until the germination is complete. Such as figure 2 , the germination rate of switchgrass seeds pretreated with melatonin was significantly higher than that of the control.
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