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Burkholderia pyrrocinia and application thereof in cercidiphyllum japonicum growth promotion

A technology of Burkholderia pyrrole and Holderia, which is applied in the fields of application, plant growth regulator, plant growth regulator, etc., can solve the problems such as no growth-promoting bacteria of A. Effects of strain resources, promotion of growth and development, and strong auxin secretion

Active Publication Date: 2016-04-13
长治学院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] At present, there are no reports on the growth-promoting bacteria of Lianxiang tree at home and abroad

Method used

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  • Burkholderia pyrrocinia and application thereof in cercidiphyllum japonicum growth promotion
  • Burkholderia pyrrocinia and application thereof in cercidiphyllum japonicum growth promotion
  • Burkholderia pyrrocinia and application thereof in cercidiphyllum japonicum growth promotion

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1: Determination of the ability of LWK2 liquid to dissolve potassium.

[0027] Potassium-solubilizing ability assay medium: sucrose 5g, MgSO 4 ·7H 2 O0.5g, Na 2 HPO 4 2g, potassium aluminosilicate 2g, FeCl 3 0.005g, pH7.0~7.5, tap water 1000mL, sterilize at 115℃ for 30min.

[0028] Inoculate a single colony of LWK2 after activation in LB (tryptone 10g, yeast extract 5g, sodium chloride 10g, deionized water 1000mL, pH 7.0, sterilize at 121°C for 20min) liquid medium, shake culture at 30°C for 18 ~24h, fully shake to form a uniform bacterial suspension, as the seed liquid. Take 0.5mL and inoculate it into a 300mL Erlenmeyer flask containing 50mL of potassium-dissolving capacity test medium, and inoculate an equal amount of LB liquid medium with the potassium-dissolving capacity test medium as a blank control. Incubate at 28°C with shaking at 200r / min for 5 days. The fermented liquid obtained is placed in a water bath and concentrated to about 10 mL, and 6% ...

Embodiment 2

[0030] Example 2: Determination of the ability of LWK2 to produce auxin.

[0031] King'sB medium: peptone 20g, MgSO 4 ·7H 2 O1.5g, K 2 HPO 4 1.5g, glycerol 10mL, agar 15g, after adjusting the pH value to 7.0, add to 1000mL.

[0032] S1 colorimetric solution: weigh 12g FeCl 3 Dissolve in 300mL deionized water, slowly add 429.7mL concentrated sulfuric acid, and dilute to 1L after cooling.

[0033] Prepare IAA standard solutions with concentrations of 1, 4, 6, 8, 10, 12, 14, 16, 18, and 20 mg / L, and mix them with S1 reagent at a volume ratio of 1:1, and place them in the dark at room temperature for 30 minutes, then Measure the OD530 value of each concentration (the 1:1 mixture of distilled water and S1 reagent is used as the blank control) value. Taking the IAA concentration as the abscissa and OD530 as the ordinate, the standard curve of IAA is obtained.

[0034] Inoculate a single colony of LWK2 in NB liquid medium for 12h (28°C, 120r / min), adjust the OD value to 0.05 w...

Embodiment 3

[0036] Example 3: Determination of the ability of LWK2 to promote growth of cucumber seeds.

[0037] Cucumber seeds were treated with 10% hydrogen peroxide for 20 minutes, rinsed with sterile water 5-6 times, and dried for later use.

[0038] Inoculate a single colony of the LWK2 strain in NB liquid medium, and culture it on a shaker at 28°C for 24-36 hours, so that the bacterial concentration reaches 10 9 cfu / mL. The bacterial suspension was serially diluted to 10 -7 , divide the bottom of the sterile petri dish into 8 equal areas with a marker pen, mark CK, -1, -2, -3, -4, -5, -6, -7 in turn, place 4 in each area ~6 pieces of sterile filter paper, put sterile absorbent cotton in the middle, put the processed cucumber seeds on the filter paper, drop 100 μl of the corresponding dilution of LWK2 bacterial suspension in the center of the filter paper in each area of ​​the bottom of the dish, and CK area An equal amount of sterile water was added dropwise as a control. 28 ℃, ...

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Abstract

The invention discloses Burkholderia pyrrocinia of which the classification name is Burkholderia pyrrocinia. The strain number is LWK2, the Burkholderia pyrrocinia is preserved in the China center for type culture collection (CCTCC), the preservation number is CCTCC NO: M 2016007, and the preservation date is January 5, 2016. The invention further discloses application of the Burkholderia pyrrocinia in cercidiphyllum japonicum growth promotion. The LWK2 strain has the strong potassium decomposing capacity and auxin secretion capacity and can obviously promote germination of cucumber seeds; when an LWK2 bactericide is inoculated to a cercidiphyllum japonicum seedling, the result shows that the bactericide can obviously promote growth of the cercidiphyllum japonicum seedling. Meanwhile, the strain has a certain antagonistic effect on phytopathogen such as pyticularia oryzae, cytospora chrysosperma, fusarium oxysporum, riziocotinia solani and rhizoctonia.

Description

technical field [0001] The invention belongs to the technical field of microbial fertilizers in the field of biological fertilizers, and in particular relates to a Rubockholderia bacterium and an application thereof in promoting the growth of sycamores. Background technique [0002] Lianxiangshu (Cercidiphyllum japonicum Sieb.EtZucc.) is a deciduous tree of the family Lianxiangshu, also known as Shanbaixiang, Zimushu, Wujushu, and Bauhinia Leafwood. Because of its aromatic taste, it can be heard from a hundred meters, and the more you shake it, the more fragrant it becomes, so it is named "Lianxiang" (Fu Liguo, 1992). Lianxiang tree is one of the only three plants in Lianxiang family. It is an ancient and rare precious deciduous tall tree, which is intermittently distributed in China and Japan. It was widely distributed in the northern hemisphere during the Cretaceous and Tertiary periods. Since the late Quaternary glacial period, the distribution area has shrunk sharply, a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01P21/00A01P3/00C12R1/01
CPCA01N63/00C12N1/205C12R2001/01
Inventor 晋婷婷白变霞任嘉红陈艳彬李世宏张鹏飞
Owner 长治学院
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