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Method of extracting and separating dihydroquercetin from roxburgh engelhardtia leaf

A technology of dihydroquercetin and wolfberry leaves, which is applied in the field of extraction and separation of dihydroquercetin, can solve the problems of low product yield, and achieve the effect of high yield and mature technology

Inactive Publication Date: 2007-10-17
宋云飞
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In the above patents, in the separation and purification step, polyamide column or liquid-liquid extraction is simply used, and then purified by recrystallization of the crude product, so the yield of the product is very low

Method used

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  • Method of extracting and separating dihydroquercetin from roxburgh engelhardtia leaf
  • Method of extracting and separating dihydroquercetin from roxburgh engelhardtia leaf

Examples

Experimental program
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Effect test

Embodiment 1

[0025] (1) Raw material processing: remove impurities from the raw material of Huang Qiye and cut into small sections of 3-5cm;

[0026] (2) Extraction and concentration: Put the processed raw materials into a multifunctional extraction tank, add 6 times of 80% ethanol for the first time, reflux extraction for 2 hours, and collect the extract; add 5 times of 80% ethanol for the second time Ethanol, reflux extraction for 2 hours, collect the extract; recover the remaining residue with ethanol for about 2 hours; combine the extracts obtained twice, concentrate under reduced pressure until there is no alcohol smell, and centrifuge to remove the precipitate;

[0027] (3) Column chromatography: Adsorb the centrifuged drug solution through a macroporous adsorption resin of type D101, wash the resin with purified water until it is colorless, and elute with 70% ethanol until the effluent is colorless, collect and combine The eluent is concentrated under reduced pressure to recover eth...

Embodiment 2

[0032] (1) Raw material processing: remove impurities from the raw material of Huang Qiye and cut into small sections of 3-5cm;

[0033] (2) Extraction and concentration: Put the processed raw materials into a multifunctional extraction tank, add 6 times of 80% ethanol for the first time, reflux extraction for 2 hours, and collect the extract; add 5 times of 80% ethanol for the second time Ethanol, reflux extraction for 2 hours, collect the extract; recover the remaining residue with ethanol for about 2 hours; combine the extracts obtained twice, concentrate under reduced pressure until there is no alcohol smell, and centrifuge to remove the precipitate;

[0034] (3) Column chromatography: Adsorb the centrifuged medicinal solution through a macroporous adsorption resin of type AB-8, wash the resin with purified water to colorless, and elute with 70% ethanol until the effluent is colorless, Collect and combine the eluate, concentrate the eluate under reduced pressure, and recov...

Embodiment 3

[0038] (1) Raw material processing: remove impurities from the raw material of Huang Qiye and cut into small sections of 3-5cm;

[0039] (2) Extraction and concentration: Put the processed raw materials into a multifunctional extraction tank, add 6 times of 80% ethanol for the first time, reflux extraction for 2 hours, and collect the extract; add 5 times of 80% ethanol for the second time Ethanol, reflux extraction for 2 hours, collect the extract; recover the remaining residue with ethanol for about 2 hours; combine the extracts obtained twice, concentrate under reduced pressure until there is no alcohol smell, and centrifuge to remove the precipitate;

[0040] (3) Column chromatography: Adsorb the centrifuged medicinal solution through a macroporous adsorption resin of NKA-II, wash the resin with purified water to colorless, and elute with 70% ethanol until the effluent is colorless, Collect and combine the eluate, concentrate the eluate under reduced pressure, and recover ...

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Abstract

The present invention discloses a method for extracting and separating dihydroquercetin from wolfberry leaf, in which dihydroquercetin is prepared from wolfberry leaf through extracting, concentrating, column chromatography, crystallizing and hydrolyzing to generate an acicular crystal white dihydroquercetin, wherein the content of dihydroquercetin more than 95%(HPLC test). The present invention can extract astilbin, quercetin and dihydroquercetin from wolfberry leaf simultaneously, which has advantages of high yield and ripe technology, and it is suitable for industrialization and continuous production.

Description

technical field [0001] The invention relates to extracting and isolating dihydroquercetin with anti-oxidative biological activity from plants, in particular to extracting and isolating dihydroquercetin from yellow bark leaves. Background technique [0002] Yellow Qi (Engelhardia Roxburghiana Wall Leaf) is a wild evergreen tree of the walnut family in Guangdong and Guangxi provinces in southern China. The leaves have been used as a health tea for clearing away heat, detoxification and weight loss since ancient times. Yellow Qiye: It has a certain effect of promoting blood circulation and removing blood stasis, lowering blood fat, lowering blood sugar and improving immune function; it has various physiological activities such as anti-oxidation, obesity inhibition, anti-allergic reaction and cancer prevention; not only for allergic reactions It is effective in treating type I allergic reactions such as rhinitis, allergic dermatitis, and bronchitis, and is also effective in trea...

Claims

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Application Information

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IPC IPC(8): C07D311/32A61K36/52A61K127/00
Inventor 宋云飞
Owner 宋云飞
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