Method of extracting and separating dihydroquercetin from roxburgh engelhardtia leaf

A technology of dihydroquercetin and wolfberry leaves, which is applied in the field of extraction and separation of dihydroquercetin, can solve the problems of low product yield, and achieve the effect of high yield and mature technology

Inactive Publication Date: 2007-10-17
宋云飞
View PDF2 Cites 30 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the above patents, in the separation and purification step, polyamide column or liquid-liquid extraction i...

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method of extracting and separating dihydroquercetin from roxburgh engelhardtia leaf
  • Method of extracting and separating dihydroquercetin from roxburgh engelhardtia leaf

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] (1) Raw material processing: remove impurities from the raw material of Huang Qiye and cut into small sections of 3-5cm;

[0026] (2) Extraction and concentration: Put the processed raw materials into a multifunctional extraction tank, add 6 times of 80% ethanol for the first time, reflux extraction for 2 hours, and collect the extract; add 5 times of 80% ethanol for the second time Ethanol, reflux extraction for 2 hours, collect the extract; recover the remaining residue with ethanol for about 2 hours; combine the extracts obtained twice, concentrate under reduced pressure until there is no alcohol smell, and centrifuge to remove the precipitate;

[0027] (3) Column chromatography: Adsorb the centrifuged drug solution through a macroporous adsorption resin of type D101, wash the resin with purified water until it is colorless, and elute with 70% ethanol until the effluent is colorless, collect and combine The eluent is concentrated under reduced pressure to recover eth...

Embodiment 2

[0032] (1) Raw material processing: remove impurities from the raw material of Huang Qiye and cut into small sections of 3-5cm;

[0033] (2) Extraction and concentration: Put the processed raw materials into a multifunctional extraction tank, add 6 times of 80% ethanol for the first time, reflux extraction for 2 hours, and collect the extract; add 5 times of 80% ethanol for the second time Ethanol, reflux extraction for 2 hours, collect the extract; recover the remaining residue with ethanol for about 2 hours; combine the extracts obtained twice, concentrate under reduced pressure until there is no alcohol smell, and centrifuge to remove the precipitate;

[0034] (3) Column chromatography: Adsorb the centrifuged medicinal solution through a macroporous adsorption resin of type AB-8, wash the resin with purified water to colorless, and elute with 70% ethanol until the effluent is colorless, Collect and combine the eluate, concentrate the eluate under reduced pressure, and recov...

Embodiment 3

[0038] (1) Raw material processing: remove impurities from the raw material of Huang Qiye and cut into small sections of 3-5cm;

[0039] (2) Extraction and concentration: Put the processed raw materials into a multifunctional extraction tank, add 6 times of 80% ethanol for the first time, reflux extraction for 2 hours, and collect the extract; add 5 times of 80% ethanol for the second time Ethanol, reflux extraction for 2 hours, collect the extract; recover the remaining residue with ethanol for about 2 hours; combine the extracts obtained twice, concentrate under reduced pressure until there is no alcohol smell, and centrifuge to remove the precipitate;

[0040] (3) Column chromatography: Adsorb the centrifuged medicinal solution through a macroporous adsorption resin of NKA-II, wash the resin with purified water to colorless, and elute with 70% ethanol until the effluent is colorless, Collect and combine the eluate, concentrate the eluate under reduced pressure, and recover ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The present invention discloses a method for extracting and separating dihydroquercetin from wolfberry leaf, in which dihydroquercetin is prepared from wolfberry leaf through extracting, concentrating, column chromatography, crystallizing and hydrolyzing to generate an acicular crystal white dihydroquercetin, wherein the content of dihydroquercetin more than 95%(HPLC test). The present invention can extract astilbin, quercetin and dihydroquercetin from wolfberry leaf simultaneously, which has advantages of high yield and ripe technology, and it is suitable for industrialization and continuous production.

Description

technical field [0001] The invention relates to extracting and isolating dihydroquercetin with anti-oxidative biological activity from plants, in particular to extracting and isolating dihydroquercetin from yellow bark leaves. Background technique [0002] Yellow Qi (Engelhardia Roxburghiana Wall Leaf) is a wild evergreen tree of the walnut family in Guangdong and Guangxi provinces in southern China. The leaves have been used as a health tea for clearing away heat, detoxification and weight loss since ancient times. Yellow Qiye: It has a certain effect of promoting blood circulation and removing blood stasis, lowering blood fat, lowering blood sugar and improving immune function; it has various physiological activities such as anti-oxidation, obesity inhibition, anti-allergic reaction and cancer prevention; not only for allergic reactions It is effective in treating type I allergic reactions such as rhinitis, allergic dermatitis, and bronchitis, and is also effective in trea...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C07D311/32A61K36/52A61K127/00
Inventor 宋云飞
Owner 宋云飞
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap