Preparation method and application of sea cucumber polypeptide chelated active calcium

Abstract

The invention discloses a preparation method and an application of sea cucumber polypeptide chelated active calcium. The preparation method comprises the following steps: softening sea cucumber through repeatedly boiling the sea cucumber by purified water, crushing the softened sea cucumber into sea cucumber particles with the diameter of 1-2mm, preparing a sea cucumber protoplasm from the sea cucumber particles, water and a composite protease, carrying out enzymatic hydrolysis for 120-180min at 45-65DEG C to obtain a sea cucumber enzymatic hydrolyzate, calcining oyster shell for 3h at 300-900DEG C, crushing the calcined oyster shell into 80-100 meshes to obtain oyster shell active calcium, mixing the sea cucumber enzymatic hydrolyzate with the oyster shell active calcium in a glass dish according to a weight ratio of 100:6-12, stirring for 10-30min, allowing the obtained product to stand for 30-40min, and drying to obtain the sea cucumber polypeptide chelated active calcium. The method has the advantages of simple preparation, convenient production, low production cost, and realization of a large amount of sea cucumber polypeptides, and the finally obtained sea cucumber polypeptide chelated active calcium has the effects of islet cell secretion improvement, and reduction of contents of glycosylated serum proteins, urea nitrogen and creatinine in blood.

Description

technical field [0001] The invention relates to the production of sea cucumber polypeptide preparations, in particular to a preparation method and application of sea cucumber polypeptide chelated active calcium. Background technique [0002] Insufficient secretion of islet cells leads to high levels of glycated serum protein, urea nitrogen, and creatinine in the blood, impaired glucose tolerance, and disordered blood lipid metabolism; increased blood sugar and blood lipids, and decreased liver and kidney functions. Sea cucumbers are rich in collagen, saponins and mucopolysaccharides, which have the functions of improving immunity, anti-tumor, lowering blood fat, anti-clotting, delaying skin aging, and reducing high blood uric acid. The publication number is CN101514354, and the title is an invention patent application for a sea cucumber polypeptide and its preparation method and application. It discloses that after the fresh sea cucumber is homogenized, it is hydrolyzed with...

AI Technical Summary

Problems solved by technology

The authorized announcement number is CN1951402, and the title is an invention patent for a production method of sea cucumber polypeptide calcium and zinc protein, which discloses that after enzymatic hydrolysis of sea cucumber pulp with trypsin, adding biological calcium and / or zinc, and drying to obtain sea cucumber polypeptide calcium and zinc Protein has the functions of supplementing calcium, improving bones, anti-radiation, detoxification, etc., but the purity of sea cucumber polypeptides in the above-mentioned sea cucumber polypeptides or containing sea cucumber polypeptide calcium and / or zinc is low, and there are many impurities such as sea cucumber polysaccharides and sea cucumber saponins, as well as pesticide residues. More, low chelation degree between sea cucumber polypeptide and calcium, zinc