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Preparation method and application of sea cucumber polypeptide chelated active calcium

A sea cucumber polypeptide and active calcium technology, applied in application, food preparation, animal protein processing and other directions, can solve the problems of low sea cucumber polypeptide purity, high pesticide residues, low chelation degree, etc. Effects of Glucose Tolerance

Inactive Publication Date: 2012-07-18
NINGBO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The authorized announcement number is CN1951402, and the title is an invention patent for a production method of sea cucumber polypeptide calcium and zinc protein, which discloses that after enzymatic hydrolysis of sea cucumber pulp with trypsin, adding biological calcium and / or zinc, and drying to obtain sea cucumber polypeptide calcium and zinc Protein has the functions of supplementing calcium, improving bones, anti-radiation, detoxification, etc., but the purity of sea cucumber polypeptides in the above-mentioned sea cucumber polypeptides or containing sea cucumber polypeptide calcium and / or zinc is low, and there are many impurities such as sea cucumber polysaccharides and sea cucumber saponins, as well as pesticide residues. More, low chelation degree between sea cucumber polypeptide and calcium, zinc

Method used

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Examples

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Effect test

Embodiment 1

[0014] A preparation method of sea cucumber polypeptide chelated active calcium, fresh sea cucumbers are removed from impurities and viscera, put into a pot filled with pure water to boil, then pour off the boiling water, add pure water to boil and then pour off the boiling water, so that the pure water can be used Boil repeatedly 4-5 times in total to obtain softened sea cucumbers, then crush the softened sea cucumbers into sea cucumber granules with a diameter of 1-2 mm, add 15 kg of water to 5 kg of sea cucumber granules, and then press pepsin, papain and neutral protease 0.125 kg of compound protease prepared with a weight ratio of 6:4:3 (the activity of each enzyme is 100,000-200,000 international units / g enzyme protein), stirred evenly to make sea cucumber puree, and at an enzymatic hydrolysis temperature of 52°C, Enzymatic hydrolysis for 177 minutes to obtain sea cucumber enzymatic hydrolysis solution; at the same time, clean oyster shells, calcinate at 300-900°C for 3 h...

Embodiment 2

[0016] It is basically the same as Example 1, except that 5 kg of sea cucumber granules is added with 10 kg of water, 0.1 kg of compound protease is added, the enzymolysis temperature is 65°C, and the enzymolysis time is 120 minutes. The activity of 100 kg of sea cucumber enzymatic solution and 6 kg of oyster shell Calcium Chelate Blend.

Embodiment 3

[0018] It is basically the same as Example 1, except that fresh sea cucumbers are replaced by water-fat sea cucumbers, 5 kg of sea cucumber granules are added with 50 kg of water, and 0.15 kg of compound protease is added. The enzymatic hydrolysis time is 180 minutes, and 100 kg of sea cucumber enzymatic hydrolyzate is mixed with 12 kg of oyster shell active calcium for chelation.

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Abstract

The invention discloses a preparation method and an application of sea cucumber polypeptide chelated active calcium. The preparation method comprises the following steps: softening sea cucumber through repeatedly boiling the sea cucumber by purified water, crushing the softened sea cucumber into sea cucumber particles with the diameter of 1-2mm, preparing a sea cucumber protoplasm from the sea cucumber particles, water and a composite protease, carrying out enzymatic hydrolysis for 120-180min at 45-65DEG C to obtain a sea cucumber enzymatic hydrolyzate, calcining oyster shell for 3h at 300-900DEG C, crushing the calcined oyster shell into 80-100 meshes to obtain oyster shell active calcium, mixing the sea cucumber enzymatic hydrolyzate with the oyster shell active calcium in a glass dish according to a weight ratio of 100:6-12, stirring for 10-30min, allowing the obtained product to stand for 30-40min, and drying to obtain the sea cucumber polypeptide chelated active calcium. The method has the advantages of simple preparation, convenient production, low production cost, and realization of a large amount of sea cucumber polypeptides, and the finally obtained sea cucumber polypeptide chelated active calcium has the effects of islet cell secretion improvement, and reduction of contents of glycosylated serum proteins, urea nitrogen and creatinine in blood.

Description

technical field [0001] The invention relates to the production of sea cucumber polypeptide preparations, in particular to a preparation method and application of sea cucumber polypeptide chelated active calcium. Background technique [0002] Insufficient secretion of islet cells leads to high levels of glycated serum protein, urea nitrogen, and creatinine in the blood, impaired glucose tolerance, and disordered blood lipid metabolism; increased blood sugar and blood lipids, and decreased liver and kidney functions. Sea cucumbers are rich in collagen, saponins and mucopolysaccharides, which have the functions of improving immunity, anti-tumor, lowering blood fat, anti-clotting, delaying skin aging, and reducing high blood uric acid. The publication number is CN101514354, and the title is an invention patent application for a sea cucumber polypeptide and its preparation method and application. It discloses that after the fresh sea cucumber is homogenized, it is hydrolyzed with...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L1/304A23L1/305A23L1/333A23L1/33A23J3/04A23J3/34A23L17/40A23L17/50
Inventor 苏秀榕李妍妍周君李晔张春丹李成华王奇
Owner NINGBO UNIV
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