Selenium-rich bifidobacterium longum as well as preparation method and application thereof

A bifidobacterium longum and selenium-enriched technology, applied in the biological field, can solve the problems of poor kidney function and poor improvement effect, and achieve the effect of lowering serum insulin level, lowering blood sugar level, and improving urine output

Active Publication Date: 2019-03-19
JIANGSU TARGET BIOMEDICINE RES INST +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to solve the shortcomings in the prior art that the improvement effect of diabetes is not good and it is easy to cause poor kidney function, the purpose of the present

Method used

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  • Selenium-rich bifidobacterium longum as well as preparation method and application thereof
  • Selenium-rich bifidobacterium longum as well as preparation method and application thereof
  • Selenium-rich bifidobacterium longum as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] (1) Preparation of bacteria solution

[0036] Bifidobacterium longum was grown anaerobically at 37°C in TPY. Sodium selenite was dissolved in 200ml TPY medium to a final concentration of 25g / mL (the concentrations of sodium selenite were tried to be 20, 22.5, 25, 27.5, 30g / mL respectively, and the effects were similar); Bifidobacterium longum Bacterial liquid, by the amount of bacterial liquid that gets: selenium-containing medium=1:25 (the mass ratio of tried Bifidobacterium longum bacterial liquid and selenium-containing medium is respectively 1:20, 1:22.5, 1:25, 1 :27.5, 1:30, with similar effects) were inoculated into new TPY medium; under anaerobic conditions, cultured overnight in a 37°C constant temperature incubator until the OD600 reached 0.2 and above, and collected the cultured bacterial liquid; at 3500rpm, Centrifuge at 4°C for 5 min, and wash 3 times with 5% glucose saline. The collected bacterial solution was resuspended in 0.1 mL of 13% skimmed milk for...

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Abstract

The invention belongs to the technical field of biology and particularly discloses selenium-rich bifidobacterium longum as well as a preparation method and application thereof. The selenium-rich bifidobacterium longum prepared by virtue of the preparation method can be applied to the preparation drugs or foods for delaying the progress of diabetes mellitus and particularly can be applied to the preparation of the drugs or the foods for improving the physical signs and metabolism conditions of diabetes mellitus, the insulin signal pathways of a diabetes mellitus model, the pathological change of livers, pancreas and kidneys of patients with diabetes mellitus and the levels of serum creatinine and blood urea nitrogen of diabetes mellitus and preventing renal complications or renal function damage of diabetes mellitus.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a selenium-enriched bifidobacterium longum, a preparation method thereof, and an application in preparing medicine or food for delaying the progress of diabetes. Background technique [0002] Bifidobacterium is a genus of bacteria that can grow naturally in the human gastrointestinal tract and protect the host against viral infection. It belongs to the genus of Gram-positive, non-motile, branched-chain anaerobic bacteria. Typically, bifidobacteria are used as probiotics to aid digestion. Scientific research today has discovered and demonstrated new uses for probiotics in metabolic disorders, including diabetes. [0003] Selenium is an essential trace element required for human and animal health, and it actively participates in animal physiological processes mainly in the form of selenoproteins. It has been reported that selenium agents can continuously improve the homeostatic metab...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/38A61K35/745A61P3/10A23L33/135A23L33/16C12R1/01
CPCA61P3/10A23L33/135A23L33/16C12N1/20C12N1/38A23V2002/00A61K2035/115A23V2200/30A23V2200/328A23V2250/1626
Inventor 华子春林艳张燕徐根兴
Owner JIANGSU TARGET BIOMEDICINE RES INST
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