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Magnetic nanoparticle compositions, and methods related thereto

a technology of magnetic nanoparticles and compositions, applied in the direction of magnetic bodies, drug compositions, metabolic disorders, etc., can solve the problem of inability to produce biocompatible magnetic nanoparticle compositions with enhanced homogeneity, and achieve the effect of enhancing homogeneity

Inactive Publication Date: 2005-12-08
NANOTX INC +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0010] In view of the above, there is a need for biocompatible magnetic nanoparticle compositions that have enhanced homogeneity and comprise high metallic content nanoparticles possessing high magnetic mobility. There is also a need for methods for producing such compositions.
[0011] It is, therefore, an aspect of the present invention to provide biocompatible magnetic nanoparticle compositions having enhanced homogeneity.

Problems solved by technology

However, a major problem has been the inability to produce biocompatible magnetic nanoparticle compositions having enhanced homogeneity, especially those having high metallic content and high magnetic mobility.

Method used

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  • Magnetic nanoparticle compositions, and methods related thereto
  • Magnetic nanoparticle compositions, and methods related thereto
  • Magnetic nanoparticle compositions, and methods related thereto

Examples

Experimental program
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Effect test

example 1

Precipitation and Analysis of Iron Oxide (Lot 146-T)

[0093] 54.1 g (0.2 mol) of FeCl3×6H2O and 31.4 g (0.158 mol) of FeCl2×4H2O were dissolved in 300 milliliter (ml) of de-ionized water. The solution of the iron salts was stirred with a mechanical stirrer at 500 revolutions per minute (rpm) in a 1 L two neck flask at room temperature. 220 ml of 25% ammonium hydroxide was added to the solution with a peristaltic pump over a period of 30 minutes (min). After addition of the ammonium hydroxide, the stirring process was continued for 10 min. The iron oxide suspension was purified via centrifugation in two 500 ml centrifuge beakers at 1100 rpm for 10 min. After removal of the supernatant, the iron oxide pellet was re-suspended two times in 200 ml of de-ionized water. The re-suspension was centrifuged three more times for successive neutralization of the iron oxide suspension. The iron oxide suspension was centrifuged again in two 500 ml beakers at 1100 rpm for 10 min. After removal of th...

example 2

Synthesis and Characterization of Dextran-Coated Nanoparticles (Lot 152-W)

[0104] 21 g of dextran (molecular weight (MW)=40.000 Daltons, available from Carl Roth, GmbH, Karlsruhe, Germany) was dissolved in 100 ml of de-ionized water. 75 ml of iron oxide suspension from Lot 146-T (concentration=45 mg / ml) was homogenized in a high-pressure-homogenizer (Fluidizer Y110, Microfluidics, Inc., Newton, Mass.) at 500 bar for 7 min. This is a pressured, air driven plunger pump that drives the fluid through a 100 micrometer (μm) diamond gap in order to force a high shear stress. The pre-heated dextran solution (about 45° C.) was added to the suspension, warmed in the homogenizer to 90° C. at 1000 bar and processed at 1000 bar for 30 min at a temperature between 87° C.-92° C. After cooling to room temperature, the nanoparticle suspension was transferred into a crystallizing dish (diameter: 12 centimeter (cm)) and placed on a permanent magnet plate for 1 hour. Then, the supernatant was transferr...

example 3

PEG-Carboxylation of Dextran-Coated Nanoparticles (Lot 202-G)

[0108] 6 ml of a suspension of dextran-coated nanoparticles of 152-W-type (example 2) with a particle concentration of 25 mg / ml in 0.1 M β-morpholino ethanesulfonic acid hydrate buffer (pH=6.3) was treated with increasing amounts of carbodiimide activated 3,6-dioxaoctanedioic acid to yield the lots of nanoparticles 202-G1-202-G4 with various PEG-COOH densities, as presented in Table V.

TABLE VTHEORETICAL PEG-COOH DENSITY OF MAGNETICNANOPARTICLESLot ID. ofAmounts of EDC, and 3,6-PEG-COOHdioxaoctanedioic acid [mgTheoretical PEG-COOHparticlesper 150 mg particle]density [nmol / mg]202-G118625202-G23.6125202-G30.7225202-G40.1445

[0109] Equal amounts (as shown in Table V) of 1-(3-dimethyl-aminopropyl)-3-ethylcarbodiimide hydrochloride (EDC) and 3,6-dioxaoctanedioic acid were dissolved in 1.5 ml of 0.5 M β-morpholino ethanesulfonic acid hydrate buffer (pH=6.3), incubated at 50° C. for 10 min, and added to the particle suspension. ...

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Abstract

Disclosed are biocompatible magnetic nanoparticle compositions for various therapeutic or biological applications, and methods related thereto. Specifically, the present invention pertains to magnetic nanoparticle compositions prepared via high-pressure homogenization processes that include a turbulent flow zone. The methods of production may involve a two-step or a one-step process. The disclosed magnetic nanoparticle compositions may be useful in the treatment of the body, body part, tissue, cell, or body fluid of a subject for a variety of indications. The disclosed magnetic nanoparticle compositions may also be useful in the fixation, separation, transportation, marking or coding of targets, or energy transformation processes.

Description

CROSS REFERENCE TO RELATED APPLICATION [0001] This is a continuation-in-part application claiming the benefit of and priority to U.S. patent application Ser. No. 10 / 360,561 filed on Feb. 6, 2003, and German patent application number 103 33 631 filed on Jul. 10, 2003, which are incorporated by reference.TECHNICAL FIELD [0002] The present invention relates generally to biocompatible magnetic nanoparticle compositions for various biological and therapeutic applications, and methods related thereto. Specifically, the present invention pertains to magnetic nanoparticle compositions prepared via high-pressure homogenization processes that include a turbulent flow zone. BACKGROUND [0003] A number of commercial applications for magnetic nanoparticles exist today. Examples include the isolation, fixation and cleaning of cells, parts of cells, nucleic acids, enzymes, antibodies, proteins or peptides; phagocyte diagnostics in cell biology; parts of diagnostic assays or therapeutic drug forms i...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): A61K33/26A61K47/48A61K49/04A61K49/18A61K51/12H01F1/00H01F1/44
CPCA61K33/26Y02A50/411A61K49/1863A61K49/1875A61K51/1255B82Y5/00B82Y25/00H01F1/445H01F1/0054Y10S977/811Y10S977/838Y10S977/83A61K47/6923A61K47/60A61K47/61A61K47/6849A61K47/6929A61K49/186A61P25/00A61P3/04A61P31/06A61P31/12A61P31/18A61P33/06A61P35/00A61P7/02A61P9/10Y02A50/30
Inventor GRUETTNER, CORDULATELLER, JOACHIMWESTPHAL, FRITZIVKOV, ROBERT
Owner NANOTX INC
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