The invention provides a
cascade viscoelastic micro-fluidic
chip and a method for preparing, sorting and enriching circulating
tumor cells. The
cascade viscoelastic micro-fluidic
chip comprises a
cell sorting module, a transition section and a
cell concentration module. The
cell sorting module is composed of an inlet I, an inlet II, an annular bifurcated
branch, a straight channel section I, an amplification section, a
serpentine channel I and an outlet I; the
cell concentration module is composed of a second straight channel section, a first bifurcation structure, a second
serpentine channel, a second outlet, a third straight channel section, a second bifurcation structure, a third
serpentine channel, a third outlet, a fourth straight channel section, a third bifurcation structure, a fourth outlet and a fifth outlet. The
cell sorting module and the
cell concentration module are cascaded through a transition section. The invention breaks through the
bottleneck that the traditional sorting technology can be compatible with downstream biological analysis only by intervening manual
centrifugation and other operations at the rear end, and can provide powerful
technical support for developing a tumor
liquid biopsy integrated
system.