Double-bioenzyme modified blending biological material containing gelatin and chitosan and preparation method and application thereof

A chitosan and biological enzyme technology, applied in the fields of medical science, absorbent pads, prostheses, etc., can solve the problems affecting the normal tissue growth of the receptor, improve the stability and mechanical properties, reduce the production cost, expand the application and Application-wide effects

Inactive Publication Date: 2014-07-02
WUHAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, although traditional chemical crosslinking can effectively improve the mechanical properties and stability of materials, due to the commonly used chemical crosslinking agents such as aldehydes (formaldehyde, glutaraldehyde and glyceraldehyde), epoxy compound components and carbodioxide Amines, etc. are often cytotoxic, which will cause biomaterials prepared by chemical cross-linking methods to affect the growth of normal tissue of the recipient after implantation.

Method used

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  • Double-bioenzyme modified blending biological material containing gelatin and chitosan and preparation method and application thereof
  • Double-bioenzyme modified blending biological material containing gelatin and chitosan and preparation method and application thereof
  • Double-bioenzyme modified blending biological material containing gelatin and chitosan and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] A method for preparing a blended biological material of gelatin and chitosan modified by dual biological enzymes, comprising the following specific steps:

[0032] 1) Preparation of solution:

[0033] Weigh 2g of gelatin and add it to 50mL of deionized water, stir in a water bath at 50°C for 1 hour until the gelatin is completely dissolved to obtain a gelatin solution with a concentration of 0.04g / mL; weigh 1g of chitosan and add it to 50mL of 1% (volume percentage v / v%) acetic acid solution, stirred at room temperature for 3 hours until the chitosan was fully dissolved to obtain a chitosan solution with a concentration of 0.02g / mL, and slowly added 1mol / L sodium hydroxide solution dropwise to the chitosan solution to The pH of the solution was 5.5; the microbial transglutaminase was added to deionized water and stirred at room temperature for 10 minutes, and then filtered to obtain a 2U / mL microbial transglutaminase solution; the tyrosinase was added to PBS (pH7. 0) ...

Embodiment 2

[0047] A method for preparing a blended biological material of gelatin and chitosan modified by dual biological enzymes, comprising the following specific steps:

[0048] 1) Preparation of solution:

[0049] Weigh 3g of gelatin and add it to 50mL of deionized water, stir in a water bath at 40°C for 1.5 hours until the gelatin is completely dissolved to obtain a gelatin solution with a concentration of 0.06g / mL; weigh 1.5g of chitosan and add it to 50mL of 1% ((volume percentage v / v%) acetic acid solution, stirred at room temperature for 4 hours until the chitosan was fully dissolved to obtain a chitosan solution with a concentration of 0.03 g / mL, and slowly added dropwise 0.5 mol / L hydroxide to the chitosan solution Sodium solution until the pH of the solution is 5.5; add microbial transglutaminase to deionized water and stir for 15 minutes, then filter to obtain a 2U / mL microbial transglutaminase solution; add tyrosinase to PBS (pH7 .0) buffer solution, stirred at room tempe...

Embodiment 3

[0055] A method for preparing a blended biological material of gelatin and chitosan modified by dual biological enzymes, comprising the following specific steps:

[0056] 1) Preparation of solution:

[0057] Weigh 6g of gelatin and add it to 100mL of deionized water, stir in a water bath at 60°C for 1.5 hours until the gelatin is completely dissolved to obtain a gelatin solution with a concentration of 0.06g / mL; weigh 2g of chitosan and add it to 100mL of 1% ((volume percentage v / v%) acetic acid solution, stirred at room temperature for 5 hours until the chitosan was fully dissolved to obtain a chitosan solution with a concentration of 0.02g / mL, and slowly added dropwise 0.8mol / L sodium hydroxide to the chitosan solution solution until the pH of the solution is 5.5; add microbial transglutaminase to deionized water and stir at room temperature for 20 minutes, then filter to obtain a 2U / mL microbial transglutaminase solution; add tyrosinase to PBS (pH7. 0) buffer solution, sti...

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Abstract

The invention provides a double-bioenzyme modified blending biological material containing gelatin and chitosan and a preparation method and application of the double-bioenzyme modified blending biological material. The blending biological material comprises gelatin and chitosan which are used a blending main body and microbial transglutaminase and tyrosinase which are used as a modifier. The stability of the blending biological material is enhanced, and the mechanical property thereof is improved; furthermore, the material has antimicrobial activity, and has good application prospect and excessively wide application in the fields of biomedical materials and a biomedicine, such as the reduction of infection and inflammatory response, antibiosis, wound protection, a biologically degradable skin dressing, a wound healing agent, artificial skin, and the like; and meanwhile, the material is simple in preparation technology, easy to control, free from side and toxic effects and temperate in preparation condition.

Description

technical field [0001] The invention belongs to the fields of biomedical materials, macromolecular chemistry and biochemistry, and in particular relates to a gelatin-chitosan blended biological material modified by dual biological enzymes and its preparation method and application. Background technique [0002] Gelatin, as a natural polymer and biomacromolecular material rich in protein sources in nature, has good biocompatibility, non-toxicity, biodegradability, can be absorbed by the human body and low price, and has been widely used in the field of biomedical materials. The application of gelatin, especially the amino acid composition and ratio of gelatin is close to that of human skin, so it has a good application prospect in skin tissue engineering such as biological dressings, wound healing agents and artificial skin [Functional Materials, 2004, 35: 2310-2313], However, the shortcomings of gelatin film, such as high brittleness, easy breakage, and easy breeding of infe...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L15/38A61L15/32A61L15/28A61L15/42A61L27/24A61L27/22A61L27/20A61L27/60
Inventor 王艺峰王燕梅吴仲岿陈艳军熊燕飞
Owner WUHAN UNIV OF TECH
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