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Cortex phellodendron chinese identification method and application thereof

An identification method, the technology of cork cortex, which is applied in the identification method of cortex cortex and its application field, can solve the problems that the cork cortex detection method cannot detect and control the quality as a whole

Inactive Publication Date: 2019-03-22
YANGTZE RIVER PHARM GRP CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The invention provides an identification method of Phellodendron, through which a large number of samples can be analyzed and processed in a short time, effectively distinguish and distinguish Phellodendron and its processed products, and counterfeit Cortex officinale, and provide methods for the quality control of Phellodendron and the division of grade quality standards Basis; Solve the problem that the detection method of Phellodendron can't detect and control its quality as a whole

Method used

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  • Cortex phellodendron chinese identification method and application thereof
  • Cortex phellodendron chinese identification method and application thereof
  • Cortex phellodendron chinese identification method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0067] Embodiment 1 Establishment method and methodological investigation of Phellodendron Phellodendron fingerprint

[0068] 1, the establishment method of Phellodendron Phellodendron fingerprint

[0069] The establishment method of Phellodendron Phellodendron fingerprint collection, comprises the following steps:

[0070] (1) Preparation of need testing solution: get 20 batches of cortex cortex from different origins in the above table 1 and pass through No. 3 pharmacopoeia sieve after pulverizing, get cortex cortex powder 0.2g, accurately weighed, place in 10mL volumetric flask, add methanol ( Containing 0.1% hydrochloric acid), set the volume to the mark, weigh, ultrasonically treat for 40min, cool to room temperature, make up the lost weight with methanol (containing 0.1% hydrochloric acid), shake well, centrifuge at 4000r / min for 10min, take the supernatant solution, through a 0.22 μm microporous membrane, and take the subsequent filtrate as the test solution;

[0071]...

Embodiment 2

[0108] Example 2 The detection and methodological investigation of the multi-wavelength quantitative components of Phellodendron Phellodendri fingerprint

[0109] 1. Detection of multi-wavelength quantitative components

[0110] The retention times of phellodine hydrochloride, magnolialine, and berberine hydrochloride were 20.173min, 20.948min, and 52.846min, respectively. Turn on the DAD detector for full-wavelength scanning, compare the peak areas of each drug efficacy index component under different wavelength conditions, and obtain the optimal detection wavelength of each index component, that is, detect the content of Phellodendron hydrochloride under the condition of 284nm wavelength, and detect the content of Phellodendron hydrochloride under the condition of 268nm wavelength For the content of magnolialine, the content of berberine hydrochloride was detected under the condition of 265nm wavelength.

[0111] 2. Research on multi-wavelength quantitative component detect...

Embodiment 3

[0154] Embodiment 3 Establishment of Phellodendron Phellodendri Chemical Pattern Recognition Method

[0155] 1. Identification of Cortex Phellodendron by Sequence Cluster Analysis (HCA)

[0156] Use the data management function of the Waters empower workstation to obtain relevant information such as the peak area and retention time of the components of Cortex Phellodendron and Cortex Phellodendri. Import the obtained data matrix into SPSS 19.0, and use the average connection pearson correlation (intra-group) connection to carry out hierarchical clustering analysis on the Phellodendron cork samples. The dendrogram is as follows Figure 4 shown. When the distance scale is about 22, Phellodendron Phellodendron and Phellodendron officinalis can be clearly distinguished, Phellodendron officinalis clustered into one type S3, Phellodendron officinalis clustered into two types S1 and S2. Through the investigation of the shape, thickness, color and texture of Phellodendron cork sampl...

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Abstract

The invention discloses a cortex phellodendron chinese identification method. According to the identification method, cortex phellodendron chinese is used for preparing a solution of a test product, solutions in which a berberine hydrochloride reference substance, a phellodendrine chloride reference substance and a magnoflorine reference substance are dissolved are used as reference substance solutions, and through high performance liquid chromatograph measurement, liquid chromatograms of the solution of the test product and the reference substance solutions are obtained respectively; the obtained liquid chromatograms are guided into a traditional Chinese medicine chromatograph fingerprint similarity evaluation system for analysis, through analysis, a cortex phellodendron chinese fingerprint is obtained, and hierarchical cluster analysis or principal component analysis or partial least square analysis is adopted for processing data of the cortex phellodendron chinese fingerprint, so that classification identification is conducted on the cortex phellodendron chinese or processed products of the cortex phellodendron chinese. According to the cortex phellodendron chinese identification method, on the basis of building the cortex phellodendron chinese fingerprint, the cortex phellodendron chinese index component content is measured, and by combining different chemical mode identification methods, the cortex phellodendron chinese, the processed products of the cortex phellodendron chinese and fake cortex phellodendri amurensis can be effectively identified and distinguished.

Description

technical field [0001] The invention relates to the field of drug analysis, in particular to an identification method of Cortex Phellodendri and its application. Background technique [0002] Phellodendron chinense, the name of traditional Chinese medicine, is the dry bark of Phellodendron chinense Schneid. It was first recorded in "Shen Nong's Materia Medica", and it is commonly called "Sichuan Phellodendron", and it is mainly produced in Sichuan, Guizhou, Hubei and other places. The main active ingredients in Cortex Phellodendri are alkaloid compounds such as berberine hydrochloride and cortex cortex hydrochloride. Phellodendron Phellodendri is bitter and cold in nature and has the functions of clearing away heat and dampness, purging fire and removing steam, detoxifying and treating sores. As a traditional Chinese medicine, it is widely used in the market and clinical treatment. At present, the specifications and grades of Phellodendron cypress on the market are not cle...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 姚仲青任晓亮陈钟曹雪晓赵倩邱峰游慧
Owner YANGTZE RIVER PHARM GRP CO LTD
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