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Process for extracting and purifying magnoflorine from garden columbine family plant

A technology of magnolia alkaloid and columbine family, which is applied in the field of membrane concentration and macroporous resin magnesium oxide mixed column chromatography combined with extraction and purification of magnolia alkaloid, can solve the problems of less development and utilization, less research on extraction and purification of active components, etc. , to achieve the effect of reducing the amount of extraction, strong specificity, and reducing damage

Inactive Publication Date: 2011-04-06
NANJING ZELANG AGRI DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, Chinese scholars have little research, development and utilization of this group of plants, mainly in the separation and identification of chemical components, and less research on the extraction and purification of their active components.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1:

[0024] Pulverize the columbine plant, put 10kg into a small extraction tank, add 80L of 5% hydrochloric acid aqueous solution, heat to 60°C for dynamic extraction at a constant temperature for 2 hours, extract 3 times, and combine the extracts. First treat the extract with a microfiltration membrane (the membrane material is a polyamide membrane), and then add an ultrafiltration membrane with a molecular weight cut-off of 2000KD. The membrane material is a polyacrylonitrile membrane, and the pressure is controlled at about 0.4Mpa. 100KD nanofiltration membrane, the membrane material is polyethersulfone membrane, get 3L of concentrated solution, add ammonia water to adjust the pH to 9.0, add 1L of ethyl acetate to extract, extract 4 times, combine the ethyl acetate extraction layer, recover ethyl acetate Esters to 1 / 15 of the total volume. Take LD605 macroporous resin and 200 mesh magnesium oxide (mass ratio is 3:1) to pack the column, add the concentrated ...

Embodiment 2

[0026] Pulverize the columbine plant, put 10kg into a small extraction tank, add 60L of 0.5% phosphoric acid aqueous solution, heat to 50°C for dynamic extraction at a constant temperature for 1 hour, extract 5 times, and combine the extracts. First treat the extract with a microfiltration membrane (the membrane material is a polyamide membrane), and then add an ultrafiltration membrane with a molecular weight cut-off of 6000KD. The membrane material is a polyethersulfone membrane, and the pressure is controlled at about 0.5Mpa. 200KD nanofiltration membrane, the membrane material is polysulfone membrane, get 3L of concentrated solution, add ammonia water to adjust the pH to 7.5, add 1.2L of ethyl acetate to extract, extract 6 times, combine the ethyl acetate extraction layer, recover ethyl acetate ester to 1 / 10 of the total volume. Get HP20 macroporous resin and 100 mesh magnesia (mass ratio is 1:2) to pack the column, add the concentrated solution to the top of the column, w...

Embodiment 3

[0028] Pulverize the columbine plant, put 20kg into a small extraction tank, add 100L of 1% acetic acid aqueous solution, heat to 80°C for dynamic extraction at a constant temperature for 1 hour, extract 4 times, and combine the extracts. First treat the extract with a microfiltration membrane (the membrane material is a polyamide membrane), and then add an ultrafiltration membrane with a molecular weight cut-off of 4000KD. The membrane material is a polyvinylidene fluoride membrane, and the pressure is controlled at about 0.2Mpa. A nanofiltration membrane with a molecular weight of 100KD, the membrane material is a polyethersulfone membrane, and 5L of the concentrated solution is obtained. Add ammonia water to the concentrated solution to adjust the pH to 8.0, add 2L of ethyl acetate to extract, extract 3 times, combine the ethyl acetate extraction layer, and recover acetic acid ethyl ester to 1 / 15 of the total volume. Take LD605 macroporous resin and 200 mesh magnesium oxide...

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Abstract

The invention relates to a process for extracting and purifying magnoflorine from a garden columbine family plant. The process comprises the steps of pulverizing a raw material, decocting in acid water, concentrating by a film, adjusting pH by alkali, extracting by ethyl acetate, carrying out macroporous resin-magnesium oxide mixed column chromatography and recrystallizing by normal hexane-acetone and drying to obtain a finished product. The method for producing the magnoflorine has the advantages of short period and low energy consumption and large preparation amount and is suitable for mass production.

Description

Technical field: [0001] The invention belongs to the technical field of biomedicine, and relates to a process for extracting and purifying magnolia alkaloids from columbine plants, specifically a process for extracting and purifying magnolan alkaloids in combination with membrane concentration and macroporous resin magnesium oxide mixed column chromatography method. Background technique: [0002] Magnoflorine (Magnoflorine), also known as Tangsongine, Magnoline, Magnoline, etc.: [0003] Molecular formula: C 20 h 24 NO 4+ ;Molecular weight: 342.41. [0004] The columbine plant belongs to the Ranunculaceae (Thalictroideae) whole herb used as medicine, has the effect of dysmenorrhea and blood circulation, folks are used to treat irregular menstruation, women's blood disorders and other diseases. There are more than 30 kinds of plants belonging to 8 genera in our country. The resources are abundant, and most of them contain a variety of physiologically active substances. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D221/18
Inventor 苏刘花
Owner NANJING ZELANG AGRI DEV
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