Pharmaceutical composition used for treating small cell carcinoma

A technology of small cell lung cancer and composition, which is applied in the field of medicine and can solve problems affecting the treatment effect and cancer cell resistance

Inactive Publication Date: 2014-07-30
QINGDAO CENT HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] At present, in the treatment of lung cancer, cisplatin is a common chemotherapeutic drug, but while it brings good curative effect, it inevitably brings serious side effects, and after a certain period of treatment, cancer cells will become resistant to it. , thus affecting the therapeutic effect of

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] The synergistic effect of the pharmaceutical composition of the present invention on the growth inhibition of human tumor cell lines in vitro

[0018] Human small cell lung cancer cell line (NCIH446) cells were suspended in the cell culture medium containing 10% (small) bovine fetal serum, 5×10 3 / well seeded in 96-well cell culture dishes. After culturing for 24 hours, cisplatin, magnolanine, puerarin or a combination of the three were added in different concentration ratios and mixed into the culture solution. MTT was measured after 72 hours of culture. Stained with tetrazolium salt (MTT) method and calculated the inhibition rate of cell proliferation when used alone or in combination. The weight proportion of composition is as follows:

[0019] weight ratio Cisplatin Magnoline Puerarin Composition 1 10 0.5 0.5 Composition 2 10 1.5 0.5 Composition 3 10 2 1 Comparative example 1 10 2 Comparative example 2 10 ...

Embodiment 2

[0026] Human small cell carcinoma cell line NCIH446 was placed in RPMI-1640 medium containing 10% fetal bovine serum at 37°C, 5% CO 2 cultivated under conditions. NCIH446 cells in the logarithmic growth phase were digested, collected and counted, and subcutaneously injected 1×10 7 NCIH446 cells, the total amount of liquid is 0.2ml, one week after inoculation of lung cancer cells in nude mice (15 in each group), intraperitoneal injection once a day for 1 week. After the last administration, the animals were killed by cervical dissection, the tumor tissue was dissected, and the tumor mass was weighed with an electronic balance, and the tumor inhibition rate was calculated according to the following formula: tumor inhibition rate=(average tumor weight of animal drinking water group-average tumor weight of experimental group ) / average tumor weight of the model group × 100%.

[0027] The specific results are as follows:

[0028] Dose (mg / kg) Tumor inhibition rate (%)...

Embodiment 3

[0030] A method similar to Example 1 was used to investigate the effect of the composition on the cisplatin-resistant lung cancer cell line A549 / DDP (purchased from Shanghai Ruilu Biotechnology Co., Ltd.), and the specific results are as follows:

[0031] Drug concentration (mg / L) Cell growth inhibition rate (%) Cisplatin (8mg) 13.2±0.8 Magnoline (8mg) 27.2±1.4 Puerarin (8mg) 2.5±0.4 Composition 1 (8mg) 79.2±3.1 Composition 2 (8mg) 88.5±1.9 Composition 3 (8mg) 92.8±3.2 Comparative example 1 (8mg) 44.2±2.4 Comparative example 2 (8mg) 42.5±3.5

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Abstract

The invention belongs to the field of medicines, and relates to a composition for inhibiting cancer cell proliferation. The invention provides a pharmaceutical composition for treating lung cancer, especially small cell carcinoma, and particularly relates to a pharmaceutical composition consisting of cis-platinum, magnoflorine and puerarin in a weight ratio of 10:(0.5-5):(0.5-5), preferably 10:(1.5-3.5):(0.5-1.5), and more preferably 10:2:1.

Description

technical field [0001] The invention belongs to the field of medicine and relates to a composition for inhibiting tumor cell proliferation. Background technique [0002] In recent years, the incidence of lung cancer, primary liver cancer, colorectal cancer, and gynecological malignant tumors has increased year by year, and has become the most common malignant tumor that seriously endangers human health, bringing many changes to our society, economy, and life. . [0003] Lung cancer is a tumor with a relatively high incidence at present. Small cell lung cancer (SCLC) is an undifferentiated cancer type of lung cancer, accounting for about 20-25% of lung cancer. Small cell lung cancer is divided into limited stage and extensive stage. Most small cell lung cancers have been diagnosed as extensive stage, and limited stage accounts for up to 1 / 3. At present, chemotherapy is the main treatment for small cell lung cancer. Most of the drugs are small molecule DNA synthesis inhibito...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K33/24A61P35/00A61K31/473A61K31/352
Inventor 王小艳王志伟李海鹰徐飞
Owner QINGDAO CENT HOSPITAL
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