Method for preparing nitidine chloride from total alkali extract of zanthoxylum nitidum stems and branches

Abstract

The invention discloses a method for preparing nitidine chloride from a total alkali extract of zanthoxylum nitidum stems and branches. The method comprises the steps: adding an alcohol-water solventwith the amount 6-12 times the mass parts of the zanthoxylum nitidum stems and branches into the zanthoxylum nitidum stems and branches, carrying out reflux extraction for 1-3 times for 1-3 h each time, merging filtrates, recovering alcohol and concentrating to a thick paste, and thus obtaining the total alkali extract of the zanthoxylum nitidum stems and branches; adding column-layer chromatographic silica gel with the amount of 2-10 times that of the thick paste to the obtained thick paste, placing in a chromatography column, carrying out solid-liquid extraction separation with ethyl acetateand methanol, merging fractions with same results by thin layer chromatography, and carrying out repeated concentration, precipitation and suction filtration, to obtain a crude compound; recrystallizing with a methanol solvent to obtain a high-purity monomer compound, carrying out primary identification of the components of the monomer compound by thin layer chromatography and a melting point determination method, and identifying the monomer compound obtained from separation to be nitidine chloride according to the physical properties of the compound and thin layer identifying. The method forextracting nitidine chloride from zanthoxylum nitidum is simple and feasible, high in yield, simple to operate and high in separation efficiency.

Description

technical field [0001] The present invention belongs to, in particular, a method for preparing chlorinated alkaloids from the total alkaloid extract of limpet stems and branches. Background technique [0002] Zanthoxylum nitidum (Roxb.) DC. Also known as double-faced needle, double-backed needle, ground golden cow, manjiao, etc., is a vine of the genus Zanthoxylum in Rutaceae, distributed in Zhejiang, Fujian, Taiwan, Hunan, Guangdong, Hainan, In Guangxi, Sichuan, Yunnan and other places, it grows in low hills, bushes, roadsides and other sunny places. The traditional medicinal part of LMZ is the root, which is a commonly used traditional Chinese medicine in clinical practice. Its nature and flavor are pungent, bitter, slightly warm, slightly poisonous, and have the effects of expelling wind and dredging collaterals, eliminating dampness and relieving pain, reducing swelling and detoxification. In traditional Chinese medicine, it can treat rheumatic paralysis, bruises, swel...

AI Technical Summary

Problems solved by technology

In recent years, with the continuous deepening of research on the chemical components and pharmacological activities of LMZ, its uses have also been continuously expanded. However, the demand for LMZ medicinal materials is also increasing, and the traditional medicinal parts are basically unable to meet the needs due to the large amount of clinical and production. Therefore, if the stems and branches of LMZ can be fully utilized, the resources of LMZ can be fully and rationally utilized, and the medicinal value and economic benefits of LMZ can be greatly improved.