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Applications of nitidine chloride in preparing medicines for preventing/treating sepsis

A technique for chloramphenicol and sepsis, which can be used in drug combinations, antipyretics, non-central analgesics, etc., can solve problems such as unreported effects, and achieve the effect of great application value

Inactive Publication Date: 2017-03-22
SHANXI ZHENDONG LEADING BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report on the effect of chlorinated dianthin on the prevention and treatment of sepsis

Method used

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  • Applications of nitidine chloride in preparing medicines for preventing/treating sepsis
  • Applications of nitidine chloride in preparing medicines for preventing/treating sepsis
  • Applications of nitidine chloride in preparing medicines for preventing/treating sepsis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1 Effects of different dosages and different administration times of dianthine chloride on the survival rate of LPS-induced sepsis mice.

[0039] 1.1 Experimental materials

[0040] Chlorinated acetine (commercially available, Shanghai Ronghe Company);

[0041] LPS (American Sigma Company, derived from Escherichia coli 055: B5);

[0042] 0.9% normal saline (made by the laboratory);

[0043] 1.2 Experimental animals

[0044] BALB / c mice, male, 6-8 weeks old, 20-22g, provided by Shanghai Slack Experimental Animal Co., Ltd., were bred in the clean animal room of the College of Pharmacy, Second Military Medical University. The temperature in the animal room was kept at 21±2°C, with a 12-hour day and night cycle, and the mice had free access to food and water. Mice were reared for 7 days to acclimate to the environment before the experiment.

[0045] 1.3 Experimental method

[0046] 1.3.1 Preparation of chlorinated acanthine samples

[0047] Weigh 10 mg, 3 mg,...

Embodiment 2

[0060] Example 2 Research experiment on the prevention and treatment of LPS-induced sepsis

[0061] 2.1 Experimental materials

[0062] 2.1.1 Drugs and reagents

[0063] Acetine Chloride (Shanghai Ronghe Company);

[0064] LPS (American Sigma Company, derived from Escherichia coli 055: B5);

[0065] 0.9% normal saline (made by the laboratory);

[0066] The ELISA kit of IL-10, TNF-α, IL-6 (U.S. eBioscience company);

[0067] Paraformaldehyde (Shanghai Boguang Company)

[0068] 2.1.2 Instruments and equipment

[0069] Horizontal centrifuge Allegra X-30R Centrifuge (Beckman coulter, USA);

[0070] -80°C ultra-low temperature refrigerator (Thermo Fisher Scientific, USA);

[0071] Constant temperature mixer (Eppendorf Thermomixer comfort, the United States);

[0072]Microplate reader microplate reader Elx800 (BioTek Instruments, USA);

[0073] 2.2 Experimental animals

[0074] BALB / c mice, male, 6-8 weeks old, 20-22g, provided by Shanghai Slack Experimental Animal Co., Lt...

Embodiment 3

[0093] Example 3 Effect of limpetine chloride on the polarization of bone marrow-derived macrophages

[0094] Under the stimulation of LPS, macrophages polarize to M1 macrophages, releasing a large amount of pro-inflammatory mediators and a small amount of anti-inflammatory mediators, but under the influence of certain factors, macrophages may transform from M1 type to RM, releasing Lower levels of pro-inflammatory mediators and higher levels of anti-inflammatory mediators. In this experiment, mouse bone marrow-derived macrophages were isolated and cultured. Firstly, the safe dose of chlorinated chlorinated on the bone marrow-derived macrophages was investigated, and then the markers of chlorinated chlorinated on M1 and RM were detected respectively. The effects of secretion and expression were used to further confirm whether acanthine chloride promotes the conversion of M1 to RM.

[0095] 2.1 Experimental materials

[0096] 2.1.1 Primary cells

[0097] Primary bone marrow-...

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Abstract

The invention provides applications of nitidine chloride in preparing medicines for preventing / treating sepsis. According to the pharmacodynamics study on the prevention and treatment for the sepsis by adopting nitidine chloride, the prevention and treatment effects of nitidine chloride on the sepsis are studied from the aspects of survival rate, histopathologic changes and serum inflammatory factors of LPS-induced sepsis mice. Nitidine chloride can obviously improve the survival rate of the sepsis mice to 80%, obviously improve the histopathologic changes of the liver and lung tissues of the sepsis mice, reduce the level of proinflammatory factors in the serum of the sepsis mice, and increase the level of anti-inflammatory factors. The cell adoptive transfer experiment proves that for the sepsis mice injected through caudal vein, the survival rate of the sepsis mice is obviously improved, and therefore, nitidine chloride can be used for preparing the medicines for preventing / treating the sepsis. The medicine provided by the invention is a medicinal composition composed of the active ingredient, namely, nitidine chloride and the pharmaceutic adjuvants, and belongs to a preparation for oral administration, injection or percutaneous drug delivery.

Description

technical field [0001] The invention relates to traditional Chinese medicines, in particular to the application of traditional Chinese medicines in the preparation of medicines, and in particular to the application of the traditional Chinese medicine monoacine chloride in the preparation of medicines for preventing / treating sepsis. Background technique [0002] Zanthoxylum nitidum (Roxb.) DC] is the dry root of Zanthoxylum nitidum (Roxb.) DC, which is mainly produced in Guangxi, Guangdong, Yunnan and other places in China. It has the effects of dispelling wind and dampness, reducing swelling and relieving pain, and is a commonly used traditional Chinese medicine among the people. It is mostly used to treat toothache, sore throat, snake bites and other diseases. It is also widely used clinically. The drugs mainly developed from LMZ include anti-rheumatic liquid, anti-tumor and pain-relieving essence, LMZ injection, etc., which are mostly used for orthopedic pain, toothache, g...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/4741A61P29/00
Inventor 张卫东单磊李明花李昆游蓉丽李建伟赵建斌
Owner SHANXI ZHENDONG LEADING BIOTECH CO LTD
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