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Method of simultaneously determining content of three active components in eight-tradtional-medicine-herb Longzuan particles

A particle and content technology, applied in the field of medicine, can solve the problems of difficulty in comprehensively and accurately controlling the quality of drugs, instability of other components, and few detection indicators, and achieves the effects of good reproducibility and stability, simple method and simple operation.

Inactive Publication Date: 2018-01-05
PHARMA FACTORY OF GUANGXI TRADITIONAL CHINESE MEDICAL UNIV
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Problems solved by technology

At present, methods for determining the content of one or two active ingredients in Baweilongzuan Granules have been disclosed in China. These methods have few detection indicators, which may lead to instability or lack of other components, and it is difficult to fully and accurately control the quality of drugs.

Method used

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  • Method of simultaneously determining content of three active components in eight-tradtional-medicine-herb Longzuan particles
  • Method of simultaneously determining content of three active components in eight-tradtional-medicine-herb Longzuan particles
  • Method of simultaneously determining content of three active components in eight-tradtional-medicine-herb Longzuan particles

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Embodiment Construction

[0021] The present invention will be further described below in conjunction with the accompanying drawings and specific embodiments, so that those skilled in the art can better understand the present invention, but the present invention is not limited thereto.

[0022] 1 Materials and Instruments

[0023] 1.1 Materials The reference substance of acanthine chloride (MUST-16030407, content 98.3%) was purchased from the Chengdu Institute of Biology, Chinese Academy of Sciences, the reference substance of psoralen (for content determination, 110739-201617) and the reference substance of hesperidin ( For content determination, 110721-201617) were all purchased from the China Institute for Food and Drug Control; 10 batches of Baweilongzuan granules were provided by the Pharmaceutical Factory of Guangxi University of Traditional Chinese Medicine; all the original medicinal materials in the experiment were provided by the Pharmaceutical Factory of Guangxi University of Traditional Chin...

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Abstract

The invention discloses a method of simultaneously determining content of hesperidin, nitidine chloride and psoralen in eight-tradtional-medicine-herb Longzuan particles through HPLC. The chromatographic conditions adopted in the method are as follow: the specification of the chromatographic column Phenomenex Gemini C18 is (250 mm*4.6 mm, 5 micrometers), the mobile phase is acetonitrile, a 0.1% phosphoric acid water solution is used for gradient elution, the flow velocity is 1 ml / min, the detection wavelength is 286 nm and 245 nm (0-30 min: 286 nm, 30-40 min:245 nm), the column temperature is30 DEG C, and the sample size is 5 micrometers. Under the chromatographic conditions, chromatographic peaks are separated well, good linear relations are formed between hesperidin, nitidine chloride and psoralen and the respective peak areas in the concentration ranges of 84.68-508.1 microgram / milliliter,16.84-101.0 microgram / milliliter and 5.504-33.02 microgram / milliliter (r values are 0.99053, 1.00000 and 0.99998 respectively), the average recovery rates are 98.1%, 99.9% and 100.1% respectively, and the RSD values are 1.3%, 2.7% and 2.5% respectively (n=6). The method is simple, rapid, accurate, high in reproducibility and capable of providing a theoretical basis for comprehensively evaluating and controlling the eight-tradtional-medicine-herb Longzuan particles.

Description

technical field [0001] The invention relates to a method for detecting active ingredients in Baweilongzuan granules, a new drug of six types of traditional Chinese medicines, in particular to a method for simultaneously measuring three active ingredients in Baweilongzuan granules, which belongs to the technical field of medicine. Background technique [0002] Rheumatoid arthritis (rheumatoid arthritis, RA) is a common chronic disease and difficult disease, which seriously endangers human health; there are many treatments for rheumatoid arthritis, including surgery, physical therapy, alternative therapy, etc. Mainly, the drugs used include immunosuppressants, regulatory anti-rheumatic drugs, non-steroidal anti-inflammatory drugs, adrenal cortex hormones, etc.; various anti-rheumatic drugs currently used in clinical practice can only relieve symptoms, but cannot fundamentally prevent the disease development, accompanied by strong toxic side effects. Therefore, it is an extrem...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
Inventor 罗宇东吴玉强谭安蔷覃洁萍陆敏灵
Owner PHARMA FACTORY OF GUANGXI TRADITIONAL CHINESE MEDICAL UNIV
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