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Application of chlorinated limpetine and its derivatives in the preparation of medicines for preventing and treating skin diseases

A technology of chlorinated amphetine and drugs, applied in the field of biomedicine, can solve problems such as low cure rate, inability to effectively prolong the remission period of patients, and slow curative effect, and achieve good safety, good application prospects, and remarkable effects

Active Publication Date: 2019-03-22
NORTHEAST NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these drugs have many disadvantages: they make the epidermis and dermis of the skin thinner, and cause toxic side effects such as telangiectasia; they can only quickly control the symptoms of patients in the progressive stage, and cannot effectively prolong the remission period of patients; Pu and Inflixid are expensive
Traditional Chinese medicines have a long history of treating psoriasis, mainly including Tripterygium wilfordii, Kunming Shanhaitang, Qingdai pills, Yujin Yinxie tablets, Yinxieling, Yinxie Granules, etc. characteristics, but the curative effect is relatively slow, the cure rate is low, and the recurrence rate is high
Therefore, there is no drug with definite curative effect in the treatment of this disease by traditional Chinese and Western medicine.

Method used

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  • Application of chlorinated limpetine and its derivatives in the preparation of medicines for preventing and treating skin diseases
  • Application of chlorinated limpetine and its derivatives in the preparation of medicines for preventing and treating skin diseases
  • Application of chlorinated limpetine and its derivatives in the preparation of medicines for preventing and treating skin diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1 Effect of dianthine chloride and its derivatives on the viability of human epidermal keratinocytes

[0025] Human epidermal keratinocytes HaCaT cells were seeded into 96-well plates and cultured at a cell density of 1.2×10 4 1 / ml, dosing after 12h, the drugs were all diluted with 1640 medium containing 3% FBS, and the negative group was used as a control with 1640 medium containing 3% FBS containing DMSO. The volume is 100 μl / well; after the cells are cultured in a 37°C incubator containing 5% CO2 for 44 hours, add 20 μl MTT (5 mg / ml) to each well and continue to culture for 4 hours; discard the supernatant, add 100 μl DMSO to each well, On the instrument, vibrate for 10 minutes, detect the OD value at 570nm, and calculate the inhibition rate:

[0026] Inhibition rate=(1-average absorbance of experimental group / average absorbance of control group)×100%.

[0027] Independent experiments were repeated more than three times, and the experimental data were calcu...

Embodiment 2

[0030] Example 2 Detection of non-toxic doses of limpetine chloride and its derivatives to subcutaneous connective tissue cells

[0031] Inoculate subcutaneous connective tissue cells A9 in a 96-well plate with a cell density of 1.2×10 4 Individual / ml; Dosing after 12h, the drugs were all diluted with DMEM medium containing 3% FBS, and the negative group was used as a control with DMEM medium containing 3% FBS containing DMSO. The volume is 100 μl / well; after the cells were cultured for 44 hours in a 37°C incubator containing 5% CO2, 20 μl of MTT (5 mg / ml) was added to each well, and the culture was continued for 4 hours; On the standard instrument, vibrate for 10 minutes, and detect the OD value at 570nm; the independent experiment was repeated more than three times, and the experimental data were all calculated with SPSS statistical software. The results are shown in Table 2, which shows that chlorinated dianthin and its derivatives have no obvious toxicity to subcutaneous ...

Embodiment 3

[0034] Example 3 Effects of dianthine chloride and its derivatives on the DNA synthesis of human epidermal keratinocytes

[0035] To further explore the effect of chlorinated chlorinated and its derivatives on the proliferation of HaCaT cells, we conducted a BrdU incorporation experiment. The HaCaT cells were treated with chlorine or its derivatives at a final concentration of 1.5 μg / ml. At the same time, the DMSO treatment group and the blank group were used as negative controls. The drug was added for 12 hours, and after being mixed with BrdU for 6 hours, a series of treatments were used. The OD value was detected at 450nm by a microplate reader.

[0036] see results figure 1 , the amount of BrdU incorporation in HaCaT cells treated with limpetine chloride or its derivatives was significantly lower than that in the DMSO control group and the blank control group, indicating that chlorinated chloramine can inhibit the proliferation of HaCaT cells by inhibiting the DNA synthes...

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PUM

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Abstract

The invention discloses nitidine chloride, a derivative thereof, and applications of nitidine chloride and the derivative of nitidine chloride in preparing medicines used for preventing and treating dermatosis. Nitidine chloride and the derivative of nitidine chloride comprise nitidine chloride, sanguinarine, and chelerythrine; the medicine can be capsules, tablets, emulsions, cream preparations, gel preparations, or ointments; and weight content of nitidine chloride and the derivative of nitidine chloride ranges from 1 to 99%. Nitidine chloride and the derivative of nitidine chloride are capable of inhibiting human skin abnormal proliferative cell proliferation; effect is excellent; and safety is high.

Description

technical field [0001] The invention belongs to the field of biomedicine, and in particular relates to the application of amphetine chloride and its derivatives in the preparation of medicines for preventing and treating skin diseases. Background technique [0002] Skin proliferative diseases refer to the benign proliferation of skin cells under the stimulation of internal and external factors, including psoriasis, common warts, periungual warts, seborrheic keratosis, flat warts, plantar warts, Melanous nevus, senile plaques, and hemangiomas were mainly located on the extremities, followed by the face and trunk, with an average of 3 skin lesions per case. Skin proliferative diseases generally do not cause serious harm to health, but they can cause pain, appearance damage, and even movement disorders, which cause great distress to patients (Joseph S.Mclaughlin, Adam B.Shafritz, MD.Cutaneous Warts[J ]. JHS, 2011, 36:342-344.). The age of onset of skin proliferative diseases ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/4741A61P17/06A61P17/08A61P17/00A61P17/12
Inventor 鲍永利李玉新杨晓光张文静
Owner NORTHEAST NORMAL UNIVERSITY
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