Method for determining nitidine chloride content in toothpaste by using double-ternary two-dimensional column high performance liquid chromatography

Abstract

The invention discloses a method for determining the nitidine chloride content in toothpaste by using the double-ternary two-dimensional column high performance liquid chromatography, belonging to the technical field of determining material content by using the high performance liquid chromatography. The method comprises the following steps of: (1) drawing a standard curve chart; (2) placing toothpaste to be tested in a centrifugal tube with a stopper and carrying out constant volume setting on the toothpaste by using methanol; (3) preparing a solution to be tested; and (4) carrying out two-dimensional column separation by using the double-ternary high performance liquid chromatography, determining nitidine chloride by using an ultraviolet detector, and obtaining the content of the nitidine chloride according to the standard curve. The method disclosed by the invention is simple and rapid to operate, good in selectivity, and good in reproducibility; and a chromatographic peak of a target analyte and an impurity chromatographic peak can achieve a better baseline separation effect, and the interference caused by interferents in a toothpaste matrix can be eliminated, therefore, the nitidine chloride in the toothpaste can be subjected to quantitative analysis accurately; and the method has a good separation effect and is wide in linear range.

Description

technical field [0001] The invention relates to a method for determining the content of acanthine chloride in toothpaste, and belongs to the technical field of high-performance liquid chromatography for determining the content of substances. Background technique [0002] As food safety incidents have aroused widespread concern in the society, some scholars and public opinion have extended their perspectives to the field of "portable" daily chemical products, and toothpaste, as an oral cleaning product, is a typical representative of this type of product, which is related to personal safety. The quality and safety supervision of functional toothpaste cannot be relaxed. Our country has a glorious and long history of research on the efficacy of Chinese herbal medicines. Chinese herbal medicine toothpaste has a history of decades since the interview, and has become the main product in the domestic toothpaste market. Some international daily chemical companies also want to join t...

AI Technical Summary

Problems solved by technology

The carbon chain of some added components is relatively long, which is difficult to remove in the pretreatment, and at the same time, it also affects the column efficiency of the chromatographic column

Use the traditional high performance liquid chromatography method to separate and detect, and the impurities in it will interfere with the determination of the standard product dihedral chloride

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