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Application of dianthin chloride in the preparation of anti-osteoporosis and bone loss diseases

A technology of chlorinated limpetine and osteoporosis, which is applied in the field of preparation of drugs with new indications

Active Publication Date: 2019-06-07
刘倩 +3
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The effect on osteoclast formation has not been reported so far

Method used

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  • Application of dianthin chloride in the preparation of anti-osteoporosis and bone loss diseases
  • Application of dianthin chloride in the preparation of anti-osteoporosis and bone loss diseases
  • Application of dianthin chloride in the preparation of anti-osteoporosis and bone loss diseases

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Experimental program
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Embodiment 1

[0027] 1. Materials and methods

[0028] Reagent

[0029] Acetine chloride was purchased from the National Institute for the Control of Pharmaceutical and Biological Products (Beijing, China). RAW 264.7 was purchased from American Type Culture Collection (Maryland, USA). Murine MCSF was purchased from R&D Systems (Minneapolis, USA). ɑ-MEM was purchased from Thermo Fisher, and fetal bovine serum (FBS) was purchased from TRACE (Sydney, Australia). Recombinant GST-rRANKL was purified in our laboratory. Recombinant human M-CSF required for human peripheral blood mononuclear cell induction was purchased from Chemicon (Garney, USA), fetal bovine serum, penicillin and L-glutamine were purchased from Invitrogen (Garney, United States). IκBα and NFATc1 antibodies were purchased from Santa Cruz Biotechnology Com. (California, USA). V-ATPase d2 antibody was synthesized and purified in our laboratory.

[0030] Isolation, Culture and Induction of Differentiation of Bone Marrow Macro...

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Abstract

The invention discloses an application of nitidine chloride in resisting osteoporosis and in preventing and treating bone loss diseases caused by osteoclasts; the inventor, through researches, discovers that the nitidine chloride can inhibit the formation of the osteoclasts and a bone absorption function in a dose-dependent mode for the first time; it discovers that the nitidine chloride, by intervening RANKL-activated NF-kB and NFAT signaling pathways, can develop an effect of inhibiting the osteoclasts; and by establishing an ovariectomized osteoporosis mouse model, it further provides the effect of the nitidine chloride on the osteoclasts. The nitidine chloride can be used as an ideal medicine for preventing and treating the bone loss diseases caused by the osteoclasts in the future.

Description

technical field [0001] The invention relates to medicines, in particular to the preparation of medicines with new indications, in particular to the application of Nitidine Chloride in the preparation of anti-osteoporosis and bone loss medicines. . Background technique [0002] Osteoporosis is a group of bone diseases caused by various reasons. The bone tissue has normal calcification, and the ratio of calcium salt to matrix is ​​normal. It is a metabolic bone lesion characterized by a decrease in the amount of bone tissue per unit volume. In most cases of osteoporosis, the loss of bone tissue is mainly due to increased bone resorption. Characterized by bone pain and susceptibility to fractures. [0003] Osteoclast is a kind of bone tissue component, which performs the function of bone resorption. Osteoclasts originate from the bone marrow hematopoietic stem cell / monocyte system and are highly differentiated multinucleated cells whose differentiation mainly depends on the ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/4741A61P19/10A61P19/08
CPCA61K31/4741
Inventor 刘倩徐家科王涛蒋伟哲赵劲民
Owner 刘倩
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