The invention discloses a respiratory syncytial
virus kit and a detection method thereof, belonging to the technical field of
enzyme immunoassay. According to the respiratory syncytial
virus kit, the pharyngeal of a child is wiped by adopting a
throat swab, nasopharyngeal secretions of the
throat swab are dissolved by an
ambroxol hydrochloride medicine, nasopharyngeal epithelial cells as sediments are added in 2.0ml of a
phosphate buffer solution containing 1.0 percent (V / V)Triton X-100 after being centrifuged at a high speed, stand for 30min, and are violently oscillated so that respiratory syncytial viruses fall off from the nasopharyngeal epithelial cells, a dissolved solution is taken to be added on a respiratory syncytial
virus detection ELISA (
Enzyme Linked Immunosorbent
Assay) plate, the respiratory syncytial viruses are detected by adopting an
ELISA method, warm bath and washing are carried out, a biotinylated respiratory syncytial virus
antibody is added,
streptavidin is coupled with a streptavidinbiotin
peroxidase complex, and a substrate liquid is added, thus a result is read. The detection method is simple to operate, is rapid, sensitive and accurate, and is low in cost, is suitable for detecting the infection of nasopharyngeal epithelial cells by the respiratory syncytial viruses, and can be used as an important inspection index of infecting the respiratory syncytial viruses by the child.