Methods for culturing and identifying nasopharyngeal carcinoma organs

Abstract

The application relates to a culture medium for nasopharyngeal carcinoma organs, and methods for culturing and identifying the nasopharyngeal carcinoma organs. According to the culture and growth characteristics of nasopharyngeal carcinoma-derived cells, a variety of cytokine components are selected and blended according to a certain ratio; the contents of cytokines and signal pathway regulatory factors in the culture medium are suitable; the nasopharyngeal carcinoma cells can effectively form organoids in a three-dimensional (3D) environment. The methods for culturing and identifying the nasopharyngeal carcinoma organs as well as the unique culture medium for the nasopharyngeal carcinoma organs can be used for successfully and stably culturing the nasopharyngeal carcinoma organs which have pathophysiological features and genotypes being highly consistent with those of the parental tumor and have high similar form of organization.

Description

technical field [0001] The invention relates to the field of organoid culture, and further relates to the field of nasopharyngeal carcinoma organoid culture, in particular to a culture medium, a culture method and an identification method for nasopharyngeal carcinoma organoids. Background technique [0002] In my country, nasopharyngeal carcinoma has become the main cancer and cause of death in malignant tumors of the ear, nose, and throat, especially in Guangdong and Guangxi in southern China (such as Guangdong, Guangxi, etc.). Radiation therapy is the first choice for nasopharyngeal carcinoma. Radiotherapy alone for stage Ⅰ and Ⅱ nasopharyngeal carcinoma can achieve a good therapeutic effect, and the 5-year overall survival rate of initial treatment can reach 75%-84.5%. However, the rate of recurrence and metastasis after the first course of treatment is as high as 10-36%. In addition, the effect of re-radiotherapy and chemotherapy is not good for patients with recurrent ...

AI Technical Summary

Problems solved by technology

In the two-dimensional culture process, it is difficult or unable to fully express the characteristics of nasopharyngeal carcinoma tissue, which makes the primary cells of cultured nasopharyngeal carcinoma tissue different from the patient's parental nasopharyngeal carcinoma tissue, which is not conducive to the research. At the same time, conventional immunohistochemistry and HE staining cannot prove that the cultured primary nasopharyngeal carcinoma cells match the genome of tumor cells in patients

In 3D culture, in the absence of necessary and suitable medium, the culture of nasopharyngeal carcinoma tissue is also unfavorable, and it is difficult to fully simulate the physiological characteristics of nasopharyngeal mucosa tissue and nasopharyngeal carcinoma tissue cells in vivo

At present, there are no studies and reports on the culture method and identification of nasopharyngeal carcinoma tissue organoids, especially the specific test process, operation steps, culture conditions, that is, the medium formula and the identification technology of nasopharyngeal carcinoma organoids.