Construction method and application of a novel animal model of hepatitis A virus

A hepatitis A virus and animal model technology, applied in the field of medical biology, can solve the problems of high cost of large animals, scarce primate sources, and high rigid conditions.

Active Publication Date: 2018-11-27
中国疾病预防控制中心病毒病预防控制所
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  • Application Information

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Problems solved by technology

The corresponding antibody to HAV can be detected in the serum of the convalescent period, but due to the scarcity of primate sources, long breeding cycle, difficult feeding and management, high price, and high requirements for laboratory hard conditions, the popularization of this model is severely limited. and applications, thus restricting people's further in-depth research on HAV
[0005] Marmots belong to Rodentia (Rodentia) Sciuridae animals. The artificial feeding management mode is similar to t

Method used

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  • Construction method and application of a novel animal model of hepatitis A virus
  • Construction method and application of a novel animal model of hepatitis A virus
  • Construction method and application of a novel animal model of hepatitis A virus

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Embodiment 1

[0048] 1. Preparation of experimental animals: breeding of marmots aged 1 to 3;

[0049] 2. Preparation and detection of the virus: Take woodchuck feces and add PBS to prepare a 1:10 (W / V) feces suspension, add chloroform with 1 / 10 of the volume of PBS, and centrifuge at 8000rpm after shaking to get the supernatant to remove solids Impurities, the supernatant was passed through a 0.22 μm filter to remove particulate impurities as much as possible, and the stool suspension was divided and stored in a -80°C refrigerator for later use.

[0050] Adopt upstream primer SEQ ID NO:9 after above-mentioned stool suspension is extracted nucleic acid; Downstream primer SEQ ID NO:10 carries out PCR amplification, and amplified product is carried out sequencing, is determined to be novel hepatitis A virus in the present invention; Primer sequence is as follows Table 3 shows:

[0051] table 3:

[0052] Primer

[0053] 3. Quantification of virus: after the nucleic acid is extracted ...

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Abstract

The invention relates to the technical field of medical biology, in particular to a building method and application of a novel hepatitis A virus animal model. The preservation number of a hepatitis A virus strain is CGMCC No. 11200, and the complete sequence of the hepatitis A virus strain is as shown in SEQ ID No. 1. The quantified hepatitis A virus strain is inoculated to an experimental animal to produce the animal model, wherein the experimental animal is a marmot and preferably a woodchuck or a Himalayan marmot. The application of the novel hepatitis A virus animal model includes application of the novel hepatitis A virus animal model to researches on hepatitis A viruses or in-vivo infection process and pathogenesis of human hepatitis A viruses and medicine screening or vaccine evaluation.

Description

technical field [0001] The invention relates to the technical field of medical biology, in particular to a method for constructing a novel animal model of hepatitis A virus and its application. Background technique [0002] Animal models are a tool for people to understand the molecular mechanism of virus replication, natural history of virus infection, virus persistent infection and pathogenesis, and to develop effective antiviral drugs and vaccines. my country is a country with severe hepatitis virus infection, and there is a great demand for animal models. Although some progress has been made in viral hepatitis with limited vaccine and therapeutic strategies, effective vaccine and therapeutic drug research is still in its infancy due to the very narrow host range of hepatitis virus and the lack of effective small animal species. As an animal model, it is used for the research of pathogens, vaccines and therapeutic drugs. [0003] Hepatitis A virus belongs to the Picorna...

Claims

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Application Information

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IPC IPC(8): A61K49/00C12Q1/52C12Q1/70C12Q1/68G01N33/569A61K39/29A61P31/14C12R1/93
CPCY02A50/30
Inventor 段招军李利利虞结梅庞立丽
Owner 中国疾病预防控制中心病毒病预防控制所
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