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Toxin-attack method for rainbow trout vaccine evaluation

A rainbow trout and vaccine technology, applied in the field of virus attack of rainbow trout vaccine evaluation, can solve the problems of poor parallelism of attack effect and inconvenient operation, achieve good absorption effect, avoid accidental injury, and ensure safety

Active Publication Date: 2020-02-18
HEILONGJIANG RIVER FISHERY RES INST CHINESE ACADEMY OF FISHERIES SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the problems of inconvenient operation and poor parallelism of the challenge effect of the existing rainbow trout vaccine evaluation method, the present invention provides a challenge method for rainbow trout vaccine evaluation

Method used

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  • Toxin-attack method for rainbow trout vaccine evaluation
  • Toxin-attack method for rainbow trout vaccine evaluation
  • Toxin-attack method for rainbow trout vaccine evaluation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] This embodiment provides a method of challenge for the rainbow trout vaccine evaluation. After the rainbow trout is anesthetized, it is placed on the rainbow trout challenge experiment operating platform to measure its body length and height, and the rainbow trout with its dorsal fin upward Measure its body width after fixation, determine the needle insertion depth of the syringe according to the size and specifications of the rainbow trout, and perform challenge injection on the back of the sterilized fish to be injected according to the set needle depth, and put the rainbow trout that has completed the challenge injection into the challenge experiment After waking up in the fish body wake-up pond set by the operating platform system, the fish body will swim back to the fish body breeding pond by itself through the pond-entry conduit.

[0034] In this example, the operations of fish body anesthesia, virus challenge and release into the culture pond are combined in an orde...

Embodiment 2

[0036] This embodiment provides a kind of rainbow trout anti-virus experimental operation platform, including fish body anesthesia pool 1, anti-virus experimental operation platform 2 and fish body recovery pool 3, and anti-virus experimental operation platform 2 is provided with fish body fixing device and fish body. Body discharge pipe mouth 18, fish body discharge pipe mouth 18 is communicated with fish body wake-up pond 3 by discharge pipe, and fish body wake-up pond 3 is communicated with fish body culture pond by entering pond conduit 22.

[0037] The fish body fixing device comprises a fixed plate 7 fixedly arranged on the operating platform 2 of the attacking virus experiment, a movable clamping plate 8 and an adjustment bottom plate 9 vertically fixed on the clamping plate 8, and the clamping plate 8 is arranged opposite to the fixed plate 7 A clamping space is formed, and the adjusting base plate 9 extends to the non-clamping side of the fixing plate 7. The adjusting ...

Embodiment 3

[0042] The present embodiment is the concrete challenge method that completes rainbow trout vaccine evaluation on the rainbow trout virus challenge experiment platform that embodiment 2 provides:

[0043] Firstly, 200 rainbow trout of 30-50 g were selected for the experiment, put them into the holding pond in the recirculating aquaculture system of rainbow trout, stopped eating for 2-3 days, and prepared before attacking the virus.

[0044] Add the anesthetic 2-phenoxyethanol in the water body of the fish body anesthesia pool, the concentration of 2-phenoxyethanol in the anesthesia pool 1 is 0.3-0.45ml / L, take 100 rainbow trout at random in the culture pool, divide 5 batches, According to the attacking injection time, the fish body anesthesia pool 1 is put in sequence for anesthesia. The rainbow trout enters anesthesia in about 2 to 3 minutes.

[0045] Take out the anesthetized rainbow trout in the anesthetized pool 1 and place it in the anesthetized fish body placement tank ...

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Abstract

The invention relates to a toxin-attack method for rainbow trout vaccine evaluation, and belongs to the technical field of aquatic product toxin-attack methods. In order to solve the problems of inconvenient operation and poor parallelism of the toxin-attack effect of the existing toxin-attack method for rainbow trout vaccine evaluation, the invention provides the toxin-attack method for rainbow trout vaccine evaluation, which includes, after anaesthetizing a rainbow trout used in the experiment, placing the rainbow trout on a toxin-attack experiment operation platform to measure body length and body height, fixing the back fin of the rainbow trout upwards, measuring the body width, determining the needle insertion depth of a syringe according to the size specification of the rainbow trout, carrying out toxin-attack injection on the part to be injected on the back of the sterilized rainbow trout, placing the rainbow trout into a fish body awakening pool, after awakening, swimming, by the rainbow trout, back to a fish body culture pool by a catheter. The method can quickly, efficiently, safely and stably complete the operations of anesthesia, toxin attack and awakening of the fish body in the rainbow trout toxin-attack experiment platform system, and does not need to carry back and forth, and thus time and labor are saved, not only the safety of the fish body can be guaranteed,but also the parallelism of the toxin-attack experiment can be ensured.

Description

technical field [0001] The invention belongs to the technical field of aquatic product challenge methods, and in particular relates to a challenge method for rainbow trout vaccine evaluation. Background technique [0002] Rainbow trout is a typical cold-water fish. Compared with other freshwater fish, it grows faster. It is favored by consumers all over the world because of its high protein, no intermuscular spines, and rich in unsaturated fatty acids such as EPA and DHA. It is more and more popular with domestic consumers. In recent years, my country's rainbow trout breeding industry has developed rapidly, but with the increase of breeding density and the deterioration of the environment, outbreaks of viral and bacterial diseases of rainbow trout are common. However, at present, there is no effective control method for rainbow trout viral diseases, and the control of bacterial diseases mainly depends on the use of antibiotics, which not only affects the healthy breeding of...

Claims

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Application Information

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IPC IPC(8): A01K61/13
CPCA01K61/13Y02A40/81
Inventor 曹永生韩世成李绍戊卢彤岩王荻徐黎明赵景壮
Owner HEILONGJIANG RIVER FISHERY RES INST CHINESE ACADEMY OF FISHERIES SCI
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