A challenge method for rainbow trout vaccine evaluation

A rainbow trout and vaccine technology, which is applied in the field of virus attack of rainbow trout vaccine evaluation, can solve the problems of poor parallelism of attack effect and inconvenient operation, and achieve good absorption effect, avoid accidental injury, and ensure parallelism

Active Publication Date: 2022-07-29
HEILONGJIANG RIVER FISHERY RES INST CHINESE ACADEMY OF FISHERIES SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the problems of inconvenient operation and poor parallelism of the challenge effect of the existing rainbow trout vaccine evaluation method, the present invention provides a challenge method for rainbow trout vaccine evaluation

Method used

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  • A challenge method for rainbow trout vaccine evaluation
  • A challenge method for rainbow trout vaccine evaluation
  • A challenge method for rainbow trout vaccine evaluation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] This example provides a challenge method for rainbow trout vaccine evaluation. After anesthetizing the rainbow trout for the test, it is placed on the rainbow trout challenge experiment operating platform to measure its body length and height, and the rainbow trout dorsal fin is upward. After fixing, measure its body width, determine the depth of the syringe needle according to the size of the rainbow trout, and carry out challenge injection to the area to be injected on the back of the sterilized fish according to the set needle depth, and the rainbow trout after the challenge injection is put into the challenge experiment. After the fish wakes up in the wake-up pool set by the operating platform system, it swims back to the fish culture pond by the entry conduit.

[0034] In this example, the operations of fish body anesthesia, poisoning and returning to the culture tank are combined in an orderly manner, and the fish body anesthesia, poisoning and awakening are convenie...

Embodiment 2

[0036] This embodiment provides a rainbow trout challenge experiment operation platform, including a fish body anesthesia pool 1, a poison challenge experiment operation platform 2 and a fish body awakening pool 3, and the fish challenge experiment operation platform 2 is provided with a fish body fixing device and a fish body. The fish body discharge pipe orifice 18 is connected with the fish body recovery pool 3 through the discharge pipe, and the fish body recovery pool 3 is communicated with the fish body culture pond through the pool inlet conduit 22 .

[0037] The fish body fixing device includes a fixed plate 7 fixedly arranged on the poisoning experiment operation platform 2, a movable clamping plate 8 and an adjustment bottom plate 9 vertically fixed to the clamping plate 8, and the clamping plate 8 is arranged opposite to the fixing plate 7 A clamping space is formed, the adjusting bottom plate 9 extends to the non-clamping side of the fixed plate 7, the adjusting bot...

Embodiment 3

[0042] The present embodiment is the concrete challenge method that completes the rainbow trout vaccine evaluation on the rainbow trout challenge experiment platform provided in Example 2:

[0043] First, 200 rainbow trout 30-50 g for the experiment were selected and placed in the temporary holding tank of the rainbow trout recirculating aquaculture system.

[0044] The anesthetic 2-phenoxyethanol was added to the water body of the fish anesthesia tank. The concentration of 2-phenoxyethanol in the anesthesia tank 1 was 0.3-0.45ml / L. 100 rainbow trout were randomly selected in the breeding tank and divided into 5 batches. According to the time of challenge injection, the fish were put into anesthesia pool 1 for anesthesia. Rainbow trout enter anesthesia in about 2 to 3 minutes.

[0045] Take out the anesthetized rainbow trout in the anesthesia tank 1 and place it in the anesthetized fish body placement tank 4, take out a fish from the anesthetized fish body placement tank 4 an...

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Abstract

The invention relates to a virus challenge method for rainbow trout vaccine evaluation, belonging to the technical field of aquatic product challenge methods. In order to solve the problems that the existing rainbow trout vaccine evaluation and challenge method is inconvenient to operate and the parallelism of the challenge effect is poor, the present invention provides a challenge method for rainbow trout vaccine evaluation. The body length and body height were measured on the operation platform of the challenge experiment, the body width was measured after the dorsal fins of the rainbow trout were fixed upward, and the depth of the syringe needle was determined according to the body size of the rainbow trout. After the rainbow trout is awake in the fish body awakening pond, it swims back to the fish body culture pond by itself through the entry conduit. The invention quickly, efficiently, safely and stably completes the operations of fish body anesthesia, challenge and recovery in the rainbow trout challenge experiment platform system, without the need for round-trip transportation, saving time and effort, and not only ensuring the safety of the fish body, but also ensuring the attack experiment. parallelism.

Description

technical field [0001] The invention belongs to the technical field of aquatic product challenge methods, and in particular relates to a challenge method for rainbow trout vaccine evaluation. Background technique [0002] Rainbow trout is a typical cold water fish, which grows faster than other freshwater fish. It is favored by consumers around the world because of its high protein, no intermuscular spines, and rich in unsaturated fatty acids such as EPA and DHA. more and more popular with domestic consumers. In recent years, my country's rainbow trout breeding industry has developed rapidly, but with the increase of breeding density and the deterioration of the environment, outbreaks of viral and bacterial diseases of rainbow trout are not uncommon. However, there is currently no effective control method for the viral diseases of rainbow trout, and the control of bacterial diseases mainly depends on the use of antibiotics, which not only affects the healthy breeding of rai...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K61/13
CPCA01K61/13Y02A40/81
Inventor 卢彤岩韩世成徐黎明赵景壮李绍戊王荻曹永生
Owner HEILONGJIANG RIVER FISHERY RES INST CHINESE ACADEMY OF FISHERIES SCI
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