Construction of virus S protein mutant pseudovirus disc, and vaccine evaluation application of virus S protein mutant pseudovirus disc

Abstract

The invention discloses construction of a virus S protein mutant pseudovirus disc and vaccine evaluation application of the virus S protein mutant pseudovirus disc. The construction comprises the following steps of: step 1, constructing a pseudovirus system; step 2, detecting the activity of the pseudovirus; and step 3, evaluating the immune effect of the SARS-CoV-2 vaccine by using the pseudovirus. In the step 1, according to a SARS-CoV-2 genome sequence number GenBank: MN908947, mutation sites with a large number of variation site strains and a wide propagation range in a database are analyzed and counted, and 40 kinds of mutation sites are selected to be used for constructing the pseudovirus. In the step 2, the activity of the S protein pseudovirus is detected, and a SARS-CoV-2 pseudovirus disc is formed after classification and statistics. In the step 3, the pseudovirus disc is applied to SARS-CoV-2 vaccine effect evaluation, the immune effect of the SARS-CoV-2 vaccine can be evaluated more comprehensively, and especially whether the existing SARS-CoV-2 vaccine can effectively prevent infection and prevalence of SARS-CoV-2 mutant strains or not is evaluated.

Description

technical field [0001] The invention relates to the technical field of vaccine evaluation, in particular to the construction of a viral S protein mutant pseudovirus disc and its vaccine evaluation application. Background technique [0002] The invasion of host cells by the new coronavirus SARS-CoV-2 is mainly mediated by the spike protein S. Mutations in the S protein will not only affect the replication and infection capabilities of the virus, but may also cause changes in the host immune response. Although the mutation frequency of SARS-CoV-2 is lower than that of other RNA viruses without reverse transcription correction function, there is currently no clear conclusion on how these mutation sites affect virus invasion of host cells. In addition, mutations in viral surface glycoproteins may cause antigenic drift (Antigenic drift), and the cross-immune protection of SARS-CoV-2 vaccines derived from parental strains is unknown. At present, the traditional vaccine effect det...

AI Technical Summary

Problems solved by technology

Currently, the global spread of SARS-CoV-2 has resulted in the generation of a large number of mutant strains

Although the recent research results on SARS-CoV-2 vaccines are gratifying, the constantly emerging mutant strains put forward higher requirements for vaccine design. In the context of the globalization of the epidemic and the rapid mutation of strains, there is a lack of a comprehensive vaccine evaluation system. The detection criteria for the effectiveness of vaccination after vaccination has not been clearly defined, so the construction of pseudoviral discs with mutant S protein of the designed virus and its application in vaccine evaluation are necessary

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