A kind of jev infectious clone with luciferase gene and its construction method and application

A technology of luciferase gene and infectious clone, applied in the field of preparation of JEV infectious clone
CN103497972BInactive Publication Date: 2015-09-30WUHAN INST OF VIROLOGY CHINESE ACADEMY OF SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
WUHAN INST OF VIROLOGY CHINESE ACADEMY OF SCI
Publication Date
2015-09-30
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a Japanese encephalitis virus (JEV) infectious clone with a luciferase gene, and a building method and application thereof. The JEV infectious clone with the luciferase gene is prepared by the following steps: (1) sectional synthesis of a JEV SA14 strain gene sequence; (2) assembling and building of the JEV infectious clone; (3) building of the JEV infectious clone with the luciferase gene. It is proved that a Rluc-JEV report virus with the same growth tendency as the JEV virus can be saved by the JEV infectious clone with the luciferase gene built by the method, and the JEV infectious clone has wide application value in the aspects of an animal model, virus replication and pathogenesis, drug screening and drug action mechanism, live animal imaging and vaccine evaluation and the like through the experiments of immunofluorescence, Rluc activity detection, virus bacteriophage plaque, drug inhibition, live animal imaging, vaccine evaluation and the like.
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Description

technical field

[0001] The invention belongs to the field of biotechnology, and in particular relates to a JEV infectious clone with a luciferase gene, a method for preparing a JEV infectious clone with a luciferase gene, and the Rluc- Application of JEV reporter virus in antiviral drug screening, animal live imaging and Japanese encephalitis vaccine evaluation. Background technique

[0002] Japanese encephalitis virus (JEV) is the pathogen of Japanese encephalitis (referred to as Japanese encephalitis). The virus is transmitted through mosquito bites. The virus enters the human body along with the mosquito saliva, causing central nervous system damage, fatality rate and fatality rate. The disability rate is high, and as a kind of infectious disease that takes children as a high-risk group, JE seriously endangers children's physical and mental health. JE is mainly prevalent in Asia, and began to spread to Australia in 1995. In recent years, the epidemic area has covered 24 ...

Claims

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