Chimeric virus of complete envelope protein of HCV (hepatitis C virus) and GB virus B

Abstract

The invention relates to the field of a virus, a compound and preparation or purification of the virus, in particular to a chimeric virus of a complete envelope protein of an HCV (hepatitis C virus) and a GB virus B. The chimeric virus is formed by sequentially connecting a 5'-end non-coding region and core protein gene sequence of the GB virus B, a complete envelope protein gene sequence of the HCV and a nonstructural protein and 3'-end non-coding region sequence of the GB virus B, wherein the complete envelope protein gene sequence of the HCV is a complete envelope protein gene sequence of a 1b-genotype HCV. A marmoset monkey can be successfully infected by the virus by carrying out intrahepatic injection and intravenous injection of a primary marmoset monkey serum containing the chimeric virus. The chimeric virus simulates infection and immune states of the HCV in a primate body. A platform for carrying out immunoassay and evaluation on the complete envelope protein of the HCV is provided. The scientific problems of limitation on HCV immunity prevention and treatment research, vaccine evaluation and the like due to shortage of a small primate infection model are solved.

Description

technical field [0001] The present invention relates to the field of virus, composition and its preparation or purification, in particular to the field of virus modified by introducing foreign gene material. Background technique [0002] Hepatitis C virus (HCV) is one of the main pathogenic factors of acute / chronic hepatitis transmitted by blood. The prevalence of HCV infection varies greatly around the world, especially in developing countries. Worldwide, approximately 170 million people are currently infected with the hepatitis C virus. 50-85% of hepatitis C virus infected patients develop into chronic hepatitis, 10-20% of them further develop into complex chronic liver diseases such as liver cirrhosis, and 1-5% develop into liver cancer after 20 or 30 years. However, there is still a lack of very effective therapeutic drugs for hepatitis C virus infection at present, and what is more serious is that there is no hepatitis C preventive vaccine in the world so far. Until t...

AI Technical Summary

Problems solved by technology

However, GB virus B is still different from hepatitis C virus, and cannot completely simulate the infection and immune status of hepatitis C virus in primates. Therefore, if the GB virus B genome is used as the backbone, the function of hepatitis C virus The gene replaces the corresponding functional region of GB virus B to construct the HCV / GBV-B chimeric virus, which is an ideal solution to study the immunity of hepatitis C virus by using the infection model

However, there are certain difficulties in replacing the complete functional protein gene of hepatitis C virus into the GB virus B genome, and there are uncertainties and risks in whether the constructed chimeric virus with complete functional protein can infect small primates Therefore, the current research using HCV / GBV-B chimeric virus has only been found in the replacement of the 5'-NCR, p7 or HVR1 of the hepatitis C virus with smaller gene segments into the GB virus B genome, which only contains C Chimeric viruses with very short gene segments of hepatitis virus can only be used to study the function of these specific gene segments, and cannot show the interaction between hepatitis C virus and the host, and cannot completely simulate the interaction of hepatitis C virus in primates immune response

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