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Humanized anti-novel coronavirus neutralizing antibody nCoV-121 and application thereof

An antibody and neutralization technology, applied in the direction of antiviral agents, antiviral immunoglobulins, applications, etc., can solve the problems of not improving the treatment process of patients and not significantly reducing the mortality rate of the new coronavirus, and achieving the effect of high antigen affinity

Active Publication Date: 2021-02-26
中国疾病预防控制中心病毒病预防控制所
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AI Technical Summary

Problems solved by technology

[0003] Although many drugs such as remdesivir, hydroxychloroquine, lopinavir and interferon beta 1a have been proved to be effective against the new crown in vitro, according to the latest WHO research, none of these drugs can significantly reduce the mortality rate of the new coronavirus, nor have they improved patient's course of treatment

Method used

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  • Humanized anti-novel coronavirus neutralizing antibody nCoV-121 and application thereof
  • Humanized anti-novel coronavirus neutralizing antibody nCoV-121 and application thereof
  • Humanized anti-novel coronavirus neutralizing antibody nCoV-121 and application thereof

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Embodiment 1

[0029] Example 1 Preparation and function of human-derived anti-new coronavirus neutralizing antibody nCoV-121

[0030] 1. Materials

[0031] 1. Samples and carriers: New coronavirus antibody-positive serum and lymphocytes: provided by Wuhan CDC and Shandong CDC. The strain XLI-Blue was purchased from Stratagene Corporation of the United States, and the antibody library construction vector pComb3H (40kb) was donated by the Scripps Research Institute of the United States.

[0032] 2. Antigen: 2019-nCoV RBD protein and S1 protein were provided by Hualan Bioengineering Co., Ltd., 2019-nCoV S protein trimer was provided by Jiangsu Dongkang Biomedical Technology Co., Ltd. RBD protein expression plasmid and S protein full-length Expression plasmids (see Chi X, Yan R, Zhang J, Zhang G, Zhang Y, Hao M, Zhang Z, Fan P, DongY, Yang Y, Chen Z, Guo Y, Zhang J, Li Y, Song X, Chen Y ,Xia L,Fu L,Hou L,Xu J,Yu C,Li J,Zhou Q,Chen W.A neutralizing human antibody binds to the N-terminal domain...

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Abstract

The invention discloses a humanized anti-novel coronavirus neutralizing antibody nCoV-121 and application thereof. The humanized neutralizing antibody nCoV-121 specifically aiming at a novel coronavirus surface antigen is successfully obtained with a phage antibody library technology, has a neutralizing function of preventing the novel coronavirus from infecting sensitive cells in vitro and is high in antigen affinity, thereby being expected to be prepared into specific antibody drugs for preventing and treating novel coronavirus pneumonia clinically.

Description

technical field [0001] The present invention relates to the field of biotechnology, in particular to human-derived anti-new coronavirus neutralizing antibody nCoV-121 and its application. Background technique [0002] The novel coronavirus SARS-CoV-2 is a new virus discovered in 2019 that can cause viral pneumonia, or lung infection, in humans. The coronavirus genome sequentially encodes the spike protein, envelope protein, membrane protein and nucleocapsid protein. Among them, the spike protein (Spike) is the most important surface membrane protein of coronavirus, which contains two subunits S1 and S2, of which S1 mainly contains the receptor binding domain (RBD), which is responsible for recognizing the receptors of cells. The SARS-CoV-2 spike protein interacts with human angiotensin-converting enzyme 2 (ACE2) to infect human respiratory epithelial cells. [0003] Although many drugs such as remdesivir, hydroxychloroquine, lopinavir and interferon beta 1a have been prove...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K16/10C12N15/13A61K39/42A61P31/14G01N33/569
CPCC07K16/10A61P31/14G01N33/56983C07K2317/56C07K2317/565C07K2317/569C07K2317/76C07K2317/92A61K2039/505G01N2333/165G01N2469/10
Inventor 梁米芳孙丽娜曲圆圆殷强玲李德新王世文李川芜为李建东李阿茜黄晓霞
Owner 中国疾病预防控制中心病毒病预防控制所
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