Porcine circovirus II-type (PCV2) epitope peptide vaccine and preparation method thereof

A porcine circovirus and epitope peptide technology, applied in the preparation method of peptide, antiviral agent, virus antigen component and other directions, can solve the problems of high price, large side effects, poor safety, etc., and achieve good immunogenicity and reliability. High, specific effect

Active Publication Date: 2014-01-29
CHONGQING UNIV OF TECH +2
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Problems solved by technology

[0010] In view of the current domestic marketed whole-virus inactivated vaccines that have a certain protective effect, but have large side effects and poor safety, as well as the imported recombinant subunit vaccines and chimeric virus ina

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  • Porcine circovirus II-type (PCV2) epitope peptide vaccine and preparation method thereof
  • Porcine circovirus II-type (PCV2) epitope peptide vaccine and preparation method thereof
  • Porcine circovirus II-type (PCV2) epitope peptide vaccine and preparation method thereof

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Embodiment Construction

[0055] The present invention will be described in further detail below in conjunction with the embodiments and accompanying drawings.

[0056] 1. Prediction and analysis of porcine circovirus type 2 CAP protein B cell epitope:

[0057] PCV2 ORF2 is the gene encoding the main structural protein of the virus-Cap protein. This protein has good antigenicity and can cause high antibody levels in infected pigs, which can be used as a candidate vaccine antigen. The present invention selects PCV2b (90% domestic PMWS pig herds are all infected by PCV2b) CAP protein as a candidate antigen, and performs B cell epitope prediction analysis on its amino acid sequence.

[0058] The amino acid sequence of the PCV2 CAP protein is as follows (deduced from the genome sequence) with a total of 233 amino acid residues, retrieved from GenBank, and the sequence number is AAF35305.1.

[0059] 1 MTYPRRRYRR RRHRPRSHGLG QILRRRPWLV HPRHRVRWRR KNGIFNTRLS RTFGVTVKRT

[0060] 61 TVRTPSWAVD MMRFNINDFL PPGG...

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Abstract

The invention relates to a porcine circovirus II-type (PCV2) epitope peptide vaccine and a preparation method of the vaccine. The vaccine contains three b cell epitopes, the lysine of the vaccine is four-branch peptide with the same core matrix structure epitope monomer and is connected with a general T ancillary cell (Th) epitope in series, and the vaccine has the molecular weight of 13kDa; the epitope peptide is named PCV CP98-156-228; after being vaccinated, a mice has stronger immune response, and a high potency virus neutralizing antibody can be generated. The epitope peptide vaccine has the advantages of being low in price, safe, high in specificity and easy to store and apply, and plays an important role in preventing and controlling the porcine circovirus disease. The PCV2 epitope peptide vaccine is prepared by four steps including predicting and screening epitope peptide, designing the epitope peptide vaccine, synthesizing, purifying and authenticating the epitope peptide as well as preparing the epitope peptide vaccine; the method is easy in raw material obtaining, lower in cost and easy to control, and has better operability, thus being suitable for being popularized and applied.

Description

technical field [0001] The invention belongs to the technical field of veterinary medicine, and in particular relates to a porcine circovirus type 2 (PCV2) epitope peptide vaccine and a preparation method thereof. [0002] Background technique [0003] 1. Porcine circovirus disease has become one of the three major diseases of pigs: [0004] Porcine circovirus type 2 (PCV2) is an important pathogen that causes post-weaning multisystemic wasting syndrome (PMWS), which was first confirmed in western Canada in 1997. The prevalence of this disease has been reported in most countries in North America, Europe and Asia. PCV2 is also associated with several other diseases, such as porcine dermatitis and nephrotic syndrome (PDNS), porcine respiratory disease syndrome (PRDC), porcine congenital tremor, sow reproductive failure, porcine hyperplastic and necrotizing pneumonia, etc. The diseases associated with PCV2 infection are collectively referred to as porcine circovirus associat...

Claims

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Application Information

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IPC IPC(8): A61K39/12C07K19/00C07K1/06C07K1/04A61P31/20
CPCY02P20/55
Inventor 吴胜昔吴玉章曾政熊仲良蔡家利梁望旺蔺露董春霞
Owner CHONGQING UNIV OF TECH
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