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Preparation method of phenolic acid-modified chitosan coating liquid for fresh keeping of edible fungus

A chitosan and edible fungus technology, applied in the field of food science, can solve the problems of low antioxidant activity, unfavorable large-scale production and application, etc., achieve high scavenging activity, improve water solubility and antioxidant activity, and have good application prospects.

Inactive Publication Date: 2017-07-25
YANGZHOU UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a kind of preparation method of the phenolic acid modified chitosan coating liquid that is used for edible fungus fresh-keeping, will have the preparation method of the phenolic acid modified chitosan of strong antioxidant activity, and synthesized Chitosan derivatives are used for fresh-keeping of edible mushroom coatings to solve the problem that the existing chitosan derivatives have low antioxidant activity and are not conducive to large-scale production and application

Method used

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  • Preparation method of phenolic acid-modified chitosan coating liquid for fresh keeping of edible fungus
  • Preparation method of phenolic acid-modified chitosan coating liquid for fresh keeping of edible fungus
  • Preparation method of phenolic acid-modified chitosan coating liquid for fresh keeping of edible fungus

Examples

Experimental program
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Effect test

Embodiment 1

[0026] The synthetic process of chitosan-protocatechuic acid graft copolymer:

[0027] (1) Weigh 0.5g chitosan and dissolve it in 25mL acetic acid solution, stir magnetically until it is completely dissolved, and simultaneously weigh 1.16g protocatechuic acid and dissolve it in 3mL absolute ethanol;

[0028] (2) Weigh 4.3g EDC and dissolve it in 20mL MES buffer, shake to dissolve EDC completely;

[0029] (3) Add the completely dissolved protocatechuic acid solution into the reactor, immediately add 0.86g NHS and adjust the pH value of the solution to 5.5, then place it in an ice-water bath, and react with magnetic stirring for 1 hour;

[0030] (4) Add the fully dissolved chitosan solution into the reactor, and react for 12 hours at 20° C.;

[0031] (5) Put the reaction solution into a dialysis bag, first dialyze with tap water for 48 hours, and then dialyze with deionized water for 24 hours;

[0032] (6) Chitosan-protocatechuic acid graft copolymer is obtained after the dial...

Embodiment 2

[0034] Structure and In Vitro Antioxidant Activity Determination of Chitosan-Protocatechuic Acid Graft Copolymer:

[0035] (1) Accurately weigh 5 mg of chitosan and chitosan-protocatechuic acid graft copolymer samples, dissolve them in 0.5 mL of heavy water, and measure their H NMR spectra at 25°C.

[0036] (2) chitosan and chitosan-protocatechuic acid graft copolymer 1 H NMR spectrum as figure 1 Shown, the peak of chitosan at δ=1.7ppm place corresponds to the methyl proton of acetylglucosamine residue, the peak at δ=3.3ppm place corresponds to the proton on the pyranose ring C-3; The peak at 3.4ppm corresponds to the proton on the pyranose ring C-4; the peak at δ=3.7ppm corresponds to the proton on the pyranose ring C-5; the peak at δ=4.3ppm corresponds to the pyranose ring Proton on C-6 of the furanose ring; the weak peak at δ=2.8 ppm corresponds to the proton on C-2; the peak at δ=4.7 ppm corresponds to the proton on C-1. However, in the chitosan-protocatechuic acid graf...

Embodiment 3

[0041] Fresh-keeping effect of chitosan-protocatechuic acid graft copolymer on edible fungi (Pleurotus eryngii).

[0042] (1) On the day of harvest, the edible fungi should be placed in a cold chain transport device at 5-8°C, with culture medium on the roots, and processed immediately after being transported to the laboratory. Discard the root culture medium, and select edible fungi with complete mushroom bodies, white color, no pests and diseases, no mechanical damage, and fruit bodies with basically the same size.

[0043] (2) Randomly divide 45 edible fungi into 3 parts, that is, 3 kinds of treatments: treat with 10g / L chitosan-protocatechuic acid graft copolymer film respectively; Membrane treatment; and the blank group without treatment. Use a polyethylene film bag seal bag with a size of 27cm×28cm to store at a humidity of 95%, and store at 4°C. Each treatment is divided into 5 groups (bags), and samples are taken every 3 days, and 3 edible fungi are randomly sampled ea...

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Abstract

The invention discloses a preparation method of a phenolic acid-modified chitosan coating liquid for fresh keeping of edible fungus. According to the preparation method, mainly chitosan and protocatechuic acid molecules are adopted as reaction substrates, and a chitosan-protocatechuic acid grafted copolymer is synthesized by using an EDC-mediated cross-linking reaction. According to the present invention, the synthesis process is simple, and the obtained chitosan-protocatechic acid grafted copolymer has characteristics of good water solubility, high grafting rate and high antioxidant activity, and can significantly improve the storage quality of edible fungus and prolong the shelf life; with the preparation method, the problems of poor water solubility, low oxidation activity and the like of the chitosan can be solved so as to improve the performance of the chitosan and expand the application in the storage and the fresh keeping of food; and the method is less investment, is suitable for large-scale production, and has excellent application prospect.

Description

technical field [0001] The invention belongs to the field of food science, and particularly relates to a preparation method of a phenolic acid-modified chitosan coating liquid for edible fungus preservation. Background technique [0002] Chitosan, also known as deacetylated chitin, is obtained through deacetylation of chitin that exists widely in nature. Chitosan is non-toxic, non-polluting and easy to degrade, and has good adsorption, film-forming and biocompatibility, and has applications in food, biology, agriculture and other fields. As a natural polysaccharide, chitosan is rich in sources, non-toxic, non-polluting, and can form a semipermeable film on the surface of food, which can effectively inhibit the invasion and growth of germs, and has attracted more and more attention. However, the poor water solubility and low antioxidant activity of natural chitosan greatly limit its application range. Therefore, chitosan needs to be modified to synthesize chitosan derivativ...

Claims

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Application Information

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IPC IPC(8): C08B37/08A23B7/154A23B7/16
CPCC08B37/003A23B7/154A23B7/16A23V2002/00A23V2200/10A23V2250/511A23V2250/02
Inventor 刘俊金昌海孟晨光阚娟严烨华单亚娜
Owner YANGZHOU UNIV
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