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Quality inspection method of Wujiaqi oral liquid (Chinese patent medicine prepared from acanthopanax root and radix astragali)

A technique for Wujiaqi oral liquid and Jiaqi oral liquid is applied in the field of quality inspection of Wujiaqi oral liquid, which can solve problems such as unfavorable quality and no quality standards, and achieve the effects of safety in clinical use and good accuracy.

Inactive Publication Date: 2015-03-18
LUOYANG HUIZHONG ANIMAL MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Wujiaqi oral liquid is a traditional Chinese medicine immune enhancer developed according to traditional Chinese medicine theory. There is no quality standard, which is not conducive to its quality control. In order to ensure safe, effective and stable clinical use, it is necessary to establish a perfect quality standard and its testing methods

Method used

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  • Quality inspection method of Wujiaqi oral liquid (Chinese patent medicine prepared from acanthopanax root and radix astragali)
  • Quality inspection method of Wujiaqi oral liquid (Chinese patent medicine prepared from acanthopanax root and radix astragali)
  • Quality inspection method of Wujiaqi oral liquid (Chinese patent medicine prepared from acanthopanax root and radix astragali)

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] Embodiment 1 prepares Acanthopanax oral liquid

[0053] Wujiaqi Oral Liquid: The prescription includes 800g of Astragalus and 200g of Acanthopanax.

[0054] Preparation method: crush the above two traditional Chinese medicines into the coarsest powder, add 8 times the amount of water, soak for 1 hour, decoct and reflux for extraction for 2 hours, filter, add water to the filter residue and extract twice, add 8 times the amount of water each time and reflux for 2 hours, Filter several times, combine the filtrates, centrifuge while hot (50-60°C), concentrate the supernatant to a relative density of 1.05-1.08 (≤70°C), refrigerate for 12-24 hours, centrifuge, and concentrate the supernatant to a relative density of 1.15 under reduced pressure ~1.23 (≤70°C), then refrigerated for 12~24h, centrifuged, added purified water to the supernatant to 1000ml, stirred well, filtered, and packaged to obtain.

Embodiment 2 5

[0055] Example 2 Examination of Specificity of Identification Method of Wujiaqi Oral Liquid

[0056] (1) Reagents and samples

[0057] Control samples: Astragaloside IV, batch number: 110781-200613, purchased from China National Institutes for Food and Drug Control;

[0058] Acanthopanax oral liquid: prepared in Example 1.

[0059] Negative control samples: Prepare negative control samples for each identification item according to the method in Example 1.

[0060] (2) Identification of Radix Astragali

[0061] Take 3ml of the sample prepared in Example 1, add 27ml of water, shake and extract 2 times with water-saturated n-butanol, 20ml each time, combine the n-butanol solution, wash 2 times with water, 20ml each time, discard the water solution, and n-butanol Evaporate the alcohol solution to dryness, add 1ml of methanol to the residue to dissolve it, and use it as the sample solution to be tested; take another control sample of astragaloside IV, add methanol to make a solu...

Embodiment 3

[0066] Example 3 Astragaloside IV content detection methodological investigation

[0067] (1) Instruments and reagents, samples

[0068] instrument:

[0069] High performance liquid chromatography, type e2695, Waters Corporation, USA; evaporative light scattering detector, type 2424, Waters Corporation, USA; electronic analytical balance, type AB135-S, Mettler Toledo Corporation.

[0070] Reagents: Acetonitrile is chromatographically pure; water is double distilled water; other reagents are analytically pure.

[0071] Control sample: Astragaloside IV, batch number: 110781-200613, purchased from China National Institutes for Food and Drug Control.

[0072] Acanthopanax oral liquid: prepared in Example 1.

[0073] (2) Chromatographic conditions

[0074] The chromatographic column is a Kromasil100-5C18 column (150×4.6mm, 5μm); the mobile phase is acetonitrile-water (35:65); detection by an evaporative light scattering detector, the temperature of the drift tube is 60°C, the t...

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Abstract

The invention provides a quality inspection method of Wujiaqi oral liquid (Chinese patent medicine prepared from acanthopanax root and radix astragali). The method comprises the processes of performing qualitative analysis and quantitative determination on the wujiaqi oral liquid, wherein the process of qualitative analysis is characterized in that thin layer chromatography is used for respectively authenticating astragaloside and isofraxidin in the wujiaqi oral liquid; the process of quantitative determination is characterized in that high performance liquid chromatography and ultraviolet-visible spectrophotometry are respectively adopted to detect the astragaloside content and contents of total sugar in the Wujiaqi oral liquid. Compared with the quality inspection method of Linqijia oral liquid (Chinese patent medicine prepared from lucid ganoderma, radix astragalus, acanthopanax root and saccharose), the inspection method disclosed by the invention is good in specificity, repeatability and accuracy, and can be used for comprehensively evaluating the quality of the Wujiaqi oral liquid, thereby effectively controlling the quality of the product, and guaranteeing the security, effectiveness and stabilization of the oral liquid in clinical use.

Description

technical field [0001] The invention belongs to the technical field of traditional Chinese medicines, and in particular relates to a quality inspection method of Wujiaqi oral liquid, which can be used for comprehensively evaluating the quality of Wujiaqi oral liquid. Background technique [0002] With the development of intensification and industrialization of the breeding industry, the susceptibility of livestock and poultry to pathogenic microorganisms is increasing, and diseases occur frequently. In order to prevent and control diseases, the traditional method is to use a large amount of antibiotics. As a result, drug-resistant strains continue to increase, resulting in livestock Poultry immune function drops sharply, growth is hindered, and the abuse of antibiotics also leads to enhanced vaccination response, increased side effects or failure of immunization, causing great economic losses to the aquaculture industry. [0003] Immunopotentiators are a class of substances ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/31G01N21/33
Inventor 张许科刘兴金张晓会
Owner LUOYANG HUIZHONG ANIMAL MEDICINE
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