Living cell vitrification freezing fluid and freezing thawing method
A technology of vitrification and freezing liquid, which is applied in the preservation, application, and animal husbandry of human or animal bodies, and can solve the problems of low blastocyst rate and low survival rate, so as to improve the survival rate and the development rate of in vitro fertilization Effect
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Embodiment 1
[0034] A vitrification solution for living cells, which is based on MEM medium and F12 medium with a volume ratio of 1:1, adding 50nmol / L isopyridine, 20nmol / L chlorogenic acid and 40μmol / L A protective agent for L; the protective agent is adenosine triphosphate.
Embodiment 2
[0036] A kind of living cell vitrification liquid, this freezing liquid is based on the volume ratio of 1:1 MEM medium and F12 medium, add 55nmol / L of isoxapyridine, 25nmol / L of chlorogenic acid and 44μmol / L A protective agent for L; the protective agent includes 22 μmol / L tea polyphenols and 22 μmol / L lentinan.
Embodiment 3
[0038] A kind of living cell vitrification liquid, this freezing liquid is based on the MEM medium and F12 medium with a volume ratio of 1:1, adding 60nmol / L of isoxapyridine, 30nmol / L of chlorogenic acid and 48μmol / L Protective agent for L; said protective agent includes 12 μmol / L hydroxyethyl starch, 10 μmol / L tea polyphenols, 10 μmol / L 4-hydroxyethylpiperazineethanesulfonic acid, 8 μmol / L lentinan and 8 μmol / L adenine nucleoside triphosphates.
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