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Technology for synchronously extracting isofraxidin and flavonoids compounds from sarcandra glabra and applications thereof

A technology of flavonoids and isopyridine, which is applied in the application, skin care preparations, medical preparations containing active ingredients, etc., can solve the problem of low product purity, low yield, and inability to extract and separate isopyridine And flavonoids and other issues, to achieve good efficacy, wide application, improve the effect of solvent recovery and utilization

Inactive Publication Date: 2010-03-10
三明华健生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The invention provides a process and application for synchronously extracting isopyridine and flavonoids from grass coral, which solves the problem that in the prior art, isopyridine and flavonoids cannot be extracted and separated from grass coral at the same time, and the purity of the separated product is It is not high, and the yield is low; the realization of simultaneous high-efficiency extraction and separation of high-purity isoxazinidine and flavonoids has certain significance for its wide application in the fields of medicine, health care, and daily cosmetics

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Chop the herbal medicinal material of coral coral into 2cm sections, use 8 times the amount of 50% methanol to extract with a frequency-rotating countercurrent extractor, control the extraction temperature at 50°C, and the flow rate of the extract is 15L / min, complete 3 countercurrent extractions, and the extraction time In 3 hours / time. The extracts are combined, first concentrated by a rising film concentration evaporator, and then concentrated by vacuum to obtain a liquid extract. The extract was extracted three times with 3 times the amount of chloroform, the supernatant was collected, concentrated, crystallized, and precipitated for later use. The crystals were dissolved with 3 times the amount of ethanol, adsorbed on a silica gel column, eluted with ethyl acetate, collected the eluate, concentrated to 10% of the original volume, cooled and crystallized, centrifuged, separated, and vacuum-dried. It was 91.3%, and the transfer rate was 62.6%. Dissolve the above pr...

Embodiment 2

[0022] Chop the grass coral medicinal material into 5cm, and extract it with a frequency-rotating countercurrent extractor. The extraction solvent is 5 times the mass concentration of 90wt% ethanol aqueous solution, the extraction temperature is controlled at 60°C, and the flow rate of the extraction liquid is 15L / min. Complete 2 times Countercurrent extraction, the extraction time is 5 hours / time. The extracts are combined, first concentrated by a rising film concentration evaporator, and then concentrated by vacuum to obtain a liquid extract. The extract is extracted three times with n-hexane of 5 times the mass, the supernatant is collected (precipitation of the extract for later use) is concentrated and then crystallized, the crystal is dissolved with 2 times the amount of methanol, adsorbed on a silica gel column, and mixed with n-hexane-ethyl acetate (n-hexane The mass ratio of alkane-ethyl acetate is 10:1) for elution, collect the eluate, concentrate to 10% of the origi...

Embodiment 3

[0024] (1) Grass coral medicinal material is crushed into 3cm before using frequency conversion countercurrent extraction.

[0025] (2) Frequency conversion countercurrent extraction is adopted, and the rising film concentration evaporator is concentrated: in the frequency conversion countercurrent extraction method, the extraction solvent is water, and the quality of the extraction solvent is 10 times the quality of the herbal coral medicinal material, and the extraction temperature is 80 ° C. , the number of extractions is 5 times, each extraction time is 5 hours, and the flow rate of the extraction liquid is 15L / min.

[0026] (3) concentrated in vacuo to obtain liquid extract: the solvent for extracting liquid extract is dichloromethane, and the extraction solvent and liquid extract mass ratio are 3: 1, and the number of extractions is 3 times, and the supernatant is collected (collecting the supernatant The subsequent liquid extract was precipitated for future use), concen...

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Abstract

The invention provides a technology for synchronously extracting isofraxidin and flavonoids compounds from sarcandra glabra and applications thereof, solving the problems that the prior art can not synchronously extract and separate isofraxidin and flavonoids compounds from sarcandra glabra, has low product purity and low productivity and the like. The technology adopts frequency rotating countercurrent extraction, obtains liquid extract by a climbing-film concentration evaporator and vacuum concentration, extracts the liquid extract and collects the liquid supernatant, crystallizes the liquidsupernatant after concentration, adds silica gel for adsorption after the crystal is dissolved, crystallizes the mixture at low temperature, and obtains the high-purity isofraxidin after vacuum drying; the liquid extract sedimentation is dissolved by adopting alkaline ethanol solution and then centrifugalized to collect the sedimentation, and the sedimentation is dissolved by water, crystallized,separated and vacuum-dried to obtain the flavonoids compounds. The technology realizes the synchronous high-efficiency extract and separation of high-purity isofraxidin and flavonoids compounds, andhas certain meaning and remarkable economic benefit on the wide application in the fields such as medicines, health care, make ups and the like.

Description

technical field [0001] The invention belongs to the technical field of coral coral extracts, and more specifically relates to a process and application for synchronously extracting isopyridine and flavonoids from coral coral. Background technique [0002] Grass coral, species alias Jiujiehua, Jiujiecha, elderberry, swollen joint wind, etc. Its medicinal parts are all herbal, pungent, bitter, and flat in nature. It has the functions of antibacterial and anti-inflammatory, clearing heat and detoxification, expelling wind and dampness, promoting blood circulation and relieving pain, promoting menstrual flow and setting bones, etc. In recent years, it has been used to treat pancreatic cancer, gastric cancer, rectal cancer, liver cancer, esophageal cancer, etc., with more significant effects. Grass coral is widely used in medicine, health care, daily cosmetics and other fields because of its unique medicinal effects, and the market demand is increasing year by year. [0003] Th...

Claims

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Application Information

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IPC IPC(8): C07D311/18A61K36/898A61K31/352A61K36/904A61K36/489A61K8/97A61K8/49A61P17/00A61P31/04A61P35/00A61Q19/10A23L2/00A23L1/302A23L33/15
Inventor 赵敏余峰陈燕梅
Owner 三明华健生物工程有限公司
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